Vol 4, No 2 (2012)


Quality of Scholarly Journals and Major Selection Criteria for Coverage by the Web of Science

Markusova V.A.


The major criteria for scholarly journals selection to be covered by the Web of Science are discussed. These criteria include the compliance of a journal with world standards, the international diversity, citation score of authors and editorial board members, the journal impact factor and journals self-citation. The demand for the unified transliteration of the authors name and the use of the unified English-language name and address of a research organization, as well as for including the information on funding organization (grant number and agency name) is emphasized.

Acta Naturae. 2012;4(2):6-13
pages 6-13 views

Publication Activity of Russian Researches in Leading International Scientific Journals

Kotsemir M.N.


The primary aim of this research report is to analyse the dynamics and structure of the publications of Russian authors, as well as to define the place of Russian science in the global scientific process. Bibliometric analysis methods are the main methods for quantitative analysis of scientific cooperation, efficiency, and other aspects of scientific activity. The information base for this research includes materials from science citation databases containing bibliographic descriptions of the articles published in scientific journals (mainly written in English) in a significant number of fields of science. Various parameters (e.g. dynamics of the number of publications, the number of citation, the level of co-authorship, the scientific specialization index, etc.) at various levels of aggregation (e.g. individual researchers, research organizations, countries and regions of the world) can be calculated based on these data. The results of bibliometric studies can be used in a number of ways: - analysis of latest trends in the development of various scientific fields; - evaluation of the effectiveness of research organizations; - overall assessment of the scientific potential of Russia (its strengths and weaknesses); - identification the most productive scientists in various fields of science; - drawing the international comparisons of publications; - analysis of collaboration networks of scientific teams. The paper analyses the basic indicators of the publication activity of scientists in Russia and the leading countries over the period between 2001 and 2011. Publication activity of Russian scientists is analysed in the context of specific areas of science. This allows the identification of areas of specialization of Russian publications. The paper also examines the dynamics of highly-cited publications and the indicators of the international scientific collaboration of Russian researchers. In this paper, materials of Web of Science database were used for analysis of publication activity.

Acta Naturae. 2012;4(2):14-34
pages 14-34 views


Physicochemical Biology: Conquered Boundaries and New Horizons

Knorre D.G.


In this paper, we shall consider the main evolutionary stages that occurred within the field of physicochemical biology during the 20th century, following the determination of the tertiary structure of DNA by Watson and Crick and the subsequent successes in the X-ray structural analysis of biopolymers. The authors’ ideas on the pre-emptive problems and the methods used in physicochemical biology in the 21st century are also presented, including an investigation of the dynamics of biochemical processes, studies of the functions of unstructured proteins, as well as single-molecule investigations of enzymatic processes and of biopolymer tertiary structure formation.

Acta Naturae. 2012;4(2):36-43
pages 36-43 views

Telomere Lengthening and Other Functions of Telomerase

Rubtsova M.P., Vasilkova D.P., Malyavko A.N., Naraikina Y.V., Zvereva M.I., Dontsova O.A.


Telomerase is an enzyme that maintains the length of the telomere. The telomere length specifies the number of divisions a cell can undergo before it finally dies (i.e. the proliferative potential of cells). For example, telomerase is activated in embryonic cell lines and the telomere length is maintained at a constant level; therefore, these cells have an unlimited fission potential. Stem cells are characterized by a lower telomerase activity, which enables only partial compensation for the shortening of telomeres. Somatic cells are usually characterized by the absence of telomerase activity. Telomere shortening leads to the attainment of the Hayflick limit, the transition of cells to a state of senescence. The cells subsequently enter a state of crisis, accompanied by massive cell death. The surviving cells become cancer cells, which are capable both of dividing indefinitely and maintaining telomere length (usually with the aid of telomerase). Telomerase is a reverse transcriptase. It consists of two major components: telomerase RNA (TER) and reverse transcriptase (TERT). TER is a non-coding RNA, and it contains the region which serves as a template for telomere synthesis. An increasing number of articles focussing on the alternative functions of telomerase components have recently started appearing. The present review summarizes data on the structure, biogenesis, and functions of telomerase.

Acta Naturae. 2012;4(2):44-61
pages 44-61 views

Coagulation Factor IX for Hemophilia B Therapy

Orlova N.A., Kovnir S.V., Vorobiev I.I., Gabibov A.G.


Factor IX is a zymogen enzyme of the blood coagulation cascade. Inherited absence or deficit of the IX functional factor causes bleeding disorder hemophilia B, which requires constant protein replacement therapy. Reviewed herein are the current state in the manufacturing of FIX, improved variants of the recombinant protein for therapy, transgenic organisms for obtaining FIX, and the advances in the gene therapy of hemophilia B.

Acta Naturae. 2012;4(2):62-73
pages 62-73 views

Research Articles

Contribution of the TGFB1 Gene to Myocardial Infarction Susceptibility

Barsova R.M., Titov B.V., Matveeva N.A., Favorov A.V., Rybalkin I.N., Vlasik T.N., Tararak E.M., Sukhinina T.S., Shahnovich R.M., Ruda M.Y., Favorova O.O.


Carriage frequencies of alleles and genotypes of the TGFB1 gene polymorphous loci -509C>T (rs1800469), 869T>C (rs1982073), 915G>C (rs1800471), which affect the level of cytokine TGF-ß1 production, were analyzed in the patients of Russian ethnic descent with myocardial infarction (MI) (406 cases) and in the control group of the same ethnic descent (198 controls). Significant association with MI was observed in carriage frequencies of the allele TGFB1*-509T (p=0.046, OR =1.45, 95% CI: 1.02-2.06), genotypes TGFB1*869T/T (p=0.0024, OR =1.75, 95% CI: 1.22-2.51), and TGFB1*915G/G (p=0.048, OR=1.76, 95% CI: 1.05-2.97). Linkage disequilibrium analysis for these SNPs has shown that the associations revealed can be considered to be independent. A complex analysis of MI association with combinations of alleles/genotypes of said SNPs indicates their cumulative effect. An analysis of susceptibility to early-onset MI (< 50 years old) revealed a positive association of the allele TGFB1*-509T (p=0.002, OR=2.24, 95% CI: 1.35-3.71) and genotype TGFB1*869T/T (p=0.008, OR=1.93, 95% CI: 1.18-3.15), as well as their additivity. An analysis of susceptibility to recurrent MI revealed an association of the genotype TGFB1*-509T/T (p=0.0078, 0R=2.60, 95% CI: 1.28-5.28). The results obtained indicate the important role of the TGFB1 gene in susceptibility to MI, including early-onset and recurrent MI, in Russians.

Acta Naturae. 2012;4(2):74-79
pages 74-79 views

Construction of a Full-Atomic Mechanistic Model of Human Apurinic/Apyrimidinic Endonuclease APE1 for Virtual Screening of Novel Inhibitors

Khaliullin I.G., Nilov D.K., Shapovalova I.V., Švedas V.K.


A full-atomic molecular model of human apurinic/apyrimidinic endonuclease APE1, an important enzyme in the DNA repair system, has been constructed. The research consisted of hybrid quantum mechanics/ molecular mechanics modeling of the enzyme-substrate interactions, as well as calculations of the ionization states of the amino acid residues of the active site of the enzyme. The choice of the APE1 mechanism with an Asp210 residue as a proton acceptor was validated by means of a generalization of modeling and experimental data. Interactions were revealed in the active site that are of greatest significance for binding the substrate and potential APE1 inhibitors (potential co-drugs of interest in the chemo- and radiotherapy of oncological diseases).

Acta Naturae. 2012;4(2):80-86
pages 80-86 views

Development of Chlamydial Type III Secretion System Inhibitors for Suppression of Acute and Chronic Forms of Chlamydial Infection

Zigangirova N.A., Zayakin E.S., Kapotina L.N., Kost E.A., Didenko L.V., Davydova D.Y., Rumyanceva J.P., Gintsburg A.L.


The Type III secretion system (T3SS) is currently considered to be one of the main pathogenicity factors in Gram-negative bacteria, which exhibit different types of parasitizing activity. The presence of this structure is essential for the development of an acute infection; the chronicity of the infection is fundamentally dependent upon its functioning. In this regard, T3TS is one of the most promising targets for the development of broad-spectrum antimicrobial drugs that do not develop resistance and are efficacious for the acute and chronic forms of infection. The mechanism of action in drug development is based on the specific inhibition of T3SS, which should interrupt the infectious process, thereby enabling the immune system to eliminate the pathogen. As a result of pilot screening using specific cellular and bacterial tests, followed by chemical optimization and detailed characterization of the biological activity, a new class of chlamydial T3SS inhibitors was obtained. The selected compounds have obvious advantages over the currently available inhibitors of T3SS pathogens thanks to the high inhibitory activity of these compounds with minimal damaging effects on eukaryotic cells. Preclinical trials of the selected inhibitors are currently under way.

Acta Naturae. 2012;4(2):87-97
pages 87-97 views

Short communications

Recombinant Human Cyclophilin A In Vitro Inhibits the Formation of Fibrin Clot

Morozov Y.A., Khromykh L.M., Dementieva I.I., Charnaia M.A., Kulikova N.L., Kazansky D.B.


The chemotactic properties of cyclophilin A are well-known. There exists however a poor level of understanding regarding the hemostatic effects of this protein. Herein it is shown that recombinant human cyclophilin A (rhСyA), in contrast to the granulocyte colony-stimulating factor, is capable of inhibiting in vitro the formation of a fibrin clot, thereby violating the spatial dynamics of clot growth; this effect is transient and dose-independent. Furthermore, the hypothesis that the conformational changes in the thrombin-rhCyA complex may mediate the anticoagulant effect of rhCyA on the autowave processes of blood clotting is postulated.

Acta Naturae. 2012;4(2):98-101
pages 98-101 views

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