Email this article Effect of 3D Cultivation Conditions on the Differentiation of Endodermal Cells
- Authors: Petrakova O.S.1,2, Ashapkin V.V.3,4, Voroteliak E.A.1, Bragin E.Y.4, Shtratnikova V.Y.4, Chernioglo E.S.1, Sukhanov Y.V.1, Terskikh V.V.1, Vasiliev A.V.1
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Affiliations:
- Koltzov Institute of Developmental Biology, Russian Academy of Sciences
- Lomonosov Moscow State University
- Belozersky Institute, Moscow State University
- Center of Innovation and Technology of Biologically Active Compounds and Their Applications, Russian Academy of Sciences
- Issue: Vol 4, No 4 (2012)
- Pages: 47-57
- Section: Research Articles
- Submitted: 17.01.2020
- Published: 15.12.2012
- URL: https://actanaturae.ru/2075-8251/article/view/10613
- DOI: https://doi.org/10.32607/20758251-2012-4-4-47-57
- ID: 10613
Cite item
Abstract
Cellular therapy of endodermal organs is one of the most important issues in modern cellular biology and biotechnology. One of the most promising directions in this field is the study of the transdifferentiation abilities of cells within the same germ layer. A method for an in vitro investigation of the cell differentiation potential (the cell culture in a three-dimensional matrix) is described in this article. Cell cultures of postnatal salivary gland cells and postnatal liver progenitor cells were obtained; their comparative analysis under 2D and 3D cultivation conditions was carried out. Both cell types have high proliferative abilities and can be cultivated for more than 20 passages. Under 2D cultivation conditions, the cells remain in an undifferentiated state. Under 3D conditions, they undergo differentiation, which was confirmed by a lower cell proliferation and by an increase in the differentiation marker expression. Salivary gland cells can undergo hepatic and pancreatic differentiation under 3D cultivation conditions. Liver progenitor cells also acquire a pancreatic differentiation capability under conditions of 3D cultivation. Thus, postnatal salivary gland cells exhibit a considerable differentiation potential within the endodermal germ layer and can be used as a promising source of endodermal cells for the cellular therapy of liver pathologies. Cultivation of cells under 3D conditions is a useful model for the in vitro analysis of the cell differentiation potential.
About the authors
O. S. Petrakova
Koltzov Institute of Developmental Biology, Russian Academy of Sciences; Lomonosov Moscow State University
Author for correspondence.
Email: PetrakovaOl@yandex.ru
Россия
V. V. Ashapkin
Belozersky Institute, Moscow State University; Center of Innovation and Technology of Biologically Active Compounds and Their Applications, Russian Academy of Sciences
Email: PetrakovaOl@yandex.ru
Россия
E. A. Voroteliak
Koltzov Institute of Developmental Biology, Russian Academy of Sciences
Email: PetrakovaOl@yandex.ru
Россия
E. Yu. Bragin
Center of Innovation and Technology of Biologically Active Compounds and Their Applications, Russian Academy of Sciences
Email: PetrakovaOl@yandex.ru
Россия
V. Yu. Shtratnikova
Center of Innovation and Technology of Biologically Active Compounds and Their Applications, Russian Academy of Sciences
Email: PetrakovaOl@yandex.ru
Россия
E. S. Chernioglo
Koltzov Institute of Developmental Biology, Russian Academy of Sciences
Email: PetrakovaOl@yandex.ru
Россия
Y. V. Sukhanov
Koltzov Institute of Developmental Biology, Russian Academy of Sciences
Email: PetrakovaOl@yandex.ru
Россия
V. V. Terskikh
Koltzov Institute of Developmental Biology, Russian Academy of Sciences
Email: PetrakovaOl@yandex.ru
Россия
A. V. Vasiliev
Koltzov Institute of Developmental Biology, Russian Academy of Sciences
Email: PetrakovaOl@yandex.ru
Россия
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