Construction of a pIX-modified Adenovirus Vector Able to Effectively Bind to Nanoantibodies for Targeting

M. N. Garas; Гарас М. Н.; S. V. Tillib; Тиллиб С. В.; O. V. Zubkova; Зубкова О. В.; V. N. Rogozhin; Рогожин В. Н.; T. I. Ivanova; Иванова Т. И.; L. A. Vasilev; Васильев Л. А.; D. Yu. Logunov; Логунов Д. Ю.; M. M. Shmarov; Шмаров М. М.; I. L. Tutykhina; Тутыхина И. Л.; I. B. Esmagambetov; Есмагамбетов И. Б.; I. Yu. Gribova; Грибова И. Ю.; A. S. Bandelyuk; Банделюк А. С.; B. S. Naroditsky; Народицкий Б. С.; A. L. Gintsburg; Гинцбург А. Л.

doi:10.32607/20758251-2014-6-2-95-105

Construction of a pIX-modified Adenovirus Vector Able to Effectively Bind to Nanoantibodies for Targeting

Authors: Garas M.N.¹, Tillib S.V.², Zubkova O.V.¹, Rogozhin V.N.¹^,3, Ivanova T.I.², Vasilev L.A.², Logunov D.Y.¹, Shmarov M.M.¹, Tutykhina I.L.¹, Esmagambetov I.B.¹, Gribova I.Y.¹, Bandelyuk A.S.¹, Naroditsky B.S.¹, Gintsburg A.L.¹
Affiliations:
1. N.F. Gamaleya Research Institute of Epidemiology and Microbiology, Ministry of Health of the Russian Federation
2. Institute of Gene Biology, Russian Academy of Sciences
3. K.I. Skryabin Moscow State Academy of Veterinary Medicine and Biotehnology
Issue: Vol 6, No 2 (2014)
Pages: 95-105
Section: Research Articles
Submitted: 17.01.2020
Published: 15.06.2014
URL: https://actanaturae.ru/2075-8251/article/view/10557
DOI: https://doi.org/10.32607/20758251-2014-6-2-95-105
ID: 10557

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Full Text

Abstract

Current targeting strategies for genetic vectors imply the creation of a specific vector for every targeted receptor, which is time-consuming and expensive. Therefore, the development of a universal vector system whose surface can specifically bind molecules to provide efficient targeting is of particular interest. In this study, we propose a new approach in creating targeted vectors based on the genome of human adenovirus serotype 5 carrying the modified gene of the capsid protein pIX (Ad5-EGFP-pIX-ER): recombinant pseudoadenoviral nanoparticles (RPANs). The surfaces of such RPANs are able to bind properly modified chimeric nanoantibodies that specifically recognize a particular target antigen (carcinoembryonic antigen (CEA)) with high affinity. The efficient binding of nanoantibodies (аСЕА-RE) to the RPAN capsid surfaces has been demonstrated by ELISA. The ability of the constructed vector to deliver target genes has been confirmed by experiments with the tumor cell lines A549 and Lim1215 expressing CEA. It has been shown that Ad5-EGFP-pIX-ER carrying аСЕА-RE on its surface penetrates into the tumor cell lines A549 and Lim1215 via the CAR-independent pathway three times more efficiently than unmodified RPAN and Ad5-EGFP-pIX-ER without nanoantibodies on the capsid surface. Thus, RPAN Ad5-EGFP-pIX-ER is a universal platform that may be useful for targeted gene delivery in specific cells due to “nanoantibody-modified RPAN” binding.

Keywords

adenoviral vector, pIX, leucine zipper, nanobody, CEA

References

Bonnekoh B., Greenhalgh D.A., Chen S.H., Block A., Rich S.S., Krieg T., Woo S.L., Roop D.R. // J Invest Dermatol. 1998, V.110, №6, P.867-871
Benihoud K., Yeh P., Perricaudet M. // Curr Opin Biotechnol. 1999, V.10, №5, P.440-447
// The Journal of Gene Medicine. Clinical Trials Database. url www.abedia.com/wiley/vectors.php
Zhang Y., Bergelson J.M. // Virology Journal 2005, V.79, №19, P.12125-12131
Vindrieux D., Le Corre L., Hsieh T.J., Metivier R., Escobar P., Caicedo A., Brigitte M., Lazennec G. // Endocr. Relat. Cancer. 2011, V.18, №3, P.311-321
Sakurai F., Mizuguchi H., Yamaguchi T., Hayakawa T. // Molecular Therapy 2003, V.8, №5, P.813-821
Breidenbach M., Rein D.T., Everts M., Glasgow J.N., Wang M., Passineau M.J. // Gene Therapy. 2005, V.12, №5, P.187-193
Korokhov N., Mikheeva G., Krendelshchikov A., Belousova N., Simonenko V., Krendelshchikova V., Pereboev A., Kotov A., Kotova O. // Virology Journal 2003, V.77, №24, P.12931-12940
Terao S., Acharya B., Suzuki T., Aoi T., Naoe M., Hamada K., Mizuguchi H., Gotoh A. // Anticancer Res. 2009, V.29, №8, P.2997-3001
Hiwasa K., Nagaya H., Terao S., Acharya B., Hamada K., Mizuguchi H., Gotoh A. // Anticancer Res. 2012, V.32, №8, P.3137-3140
Hongju W., Han T., Belousova N., Krasnykh V., Kashentseva E., Dmitriev I., Kataram M., Mahasreshti P. J., Curiel D. T. // Virology Journal 2005, V.79, №6, P.3382-3390
Vigne E., Mahfouz I., Dedieu J.F., Brie A., Perricaudet M., Yeh P. // Virology Journal 1999, V.73, №6, P.5156-5161
Dmitriev I.P., Kashentseva E.A., Curiel D.T. // Virology Journal 2002, V.76, №14, P.6893-6899
Vellinga J., Van der Heijdt S., Hoeben R.C. // J Gen Virol. 2005, V.86, №6, P.1581-1588
Davison E., Diaz R.M., Hart I.R., Santis G., Marshall J.F. // Virology Journal 1997, V.71, №80, P.6204-6207
Iyer S.V., Davis D.L., Seal S.N., Burch J.B. // Mol Cell Biol. 1991, V.11, №10, P.4863-4875
Moll J.R., Ruviniv S.B., Pastan I., Vinson C. // Protein Science. 2001, V.10, P.649-655
Glasgow J.N., Mikheeva G., Krasnykh V., Curiel D.T. // PLoS One. 2009, V.4, №12, P.8355
Tillib S.V. // Molecular biology. 2011, V.45, P.77-85
Tillib S., Ivanova T.I., Vasilev L.A., Rutovskaya M.V., Saakyan S.A., Gribova I.Y., Tutykhina I.L., Sedova E.S., Lysenko A.A., Shmarov M.M., Logunov D.Y., Naroditsky B.S., Gintsburg A.L. // Antiviral Research. 2013, V.97, P.245-254
Gribova I.Y., Tillib S.V., Tutykhina I.L., Shmarov M.M., Logunov D.Y., Verkhovskaia L.V., Naroditsky B.S., Gintsburg A.L. // Acta Naturae. 2011, V.3, P.66-72
Tutykhina I., Sedova E., Gribova I., Ivanova T.I., Vasilev L.A., Rutovskaya M.V., Lysenko A., Shmarov M., Logunov D., Naroditsky B., Tillib S., Gintsburg A. // Antiviral Research. 2013, V.97, P.717-720
Shmarov M.M., Cherenova L.V., Shashkova E.V., Logunov D.U., Verkhovskaia L.V., Kapitonov A.V., Neugodova G.L., Doronin K.K., Naroditski B.S. // Mol. Gen. Mikrobiol. Virusol. 2002, V.2, P.30-35
Hamers-Casterman C., Atarhouch T., Muyldermans S. // Nature 1993, V.363, P.446-448
Nguyen V.K., Desmyter A., Muyldermans S. // Adv. Immunol. 2001, V.79, P.261-296
Saerens D., Kinne J., Bosmans E., Wernery U., Muyldermans S., Conrath K. J. // Biol Chem. 2004, V.279, P.51965-51972
Rothbauer U., Zolghadr K., Tillib S. // Nature Methods. 2006, V.3, P.887-889
Tillib S., Ivanova T.I., Vasilev L.A. // Acta Naturae. 2010, V.2(3), P.100-108
Ghosh-Choudhury G., Haj-Ahmad Y., Graham F.L. // EMBO J. 1987, V.6, №6, P.1733-1739
Vellinga J., Rabelink M.J., Cramer S.J., van den Wollenberg D.J., Van der Meulen H., Leppard K.N., Fallaux F.J., Hoeben R.C. // Virology Journal 2004, V.78, №7, P.3470-3479
Boulanger P., Lemay P., Blair G.E., Russell W.C. // J. Gen. Virol. 1979, V.44, №3, P.783-800
Mathis J.M., Bhatia S., Khandelwal A., Kovesdi I., Lokitz S.J., Odaka Y., Takalkar A.M., Terry T., Curiel D.T. // PLoS One. 2011, V.6, №2, P.16792
Campos S.K., Parrott M.B., Barry M.A. // Molecular Therapy 2004, V.9, №6, P.942-954
Tang Y., Le L.P., Matthews Q.L., Han T., Wu H., Curiel D.T. // Virology 2008, V.377, №2, P.391-400
Davison E., Kirby I., Whitehouse J., Hart I., Marshall J.F., Santis G. // J Gene Med. 2001, V.3, №6, P.550-559
Simpson H.D., Barras F. // Journal of Bacteriology 1999, V.181, №15, P.4611-4616

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Construction of a pIX-modified Adenovirus Vector Able to Effectively Bind to Nanoantibodies for Targeting

Full Text

Abstract

Keywords

About the authors

M. N. Garas

S. V. Tillib

O. V. Zubkova

V. N. Rogozhin

T. I. Ivanova

L. A. Vasilev

D. Yu. Logunov

M. M. Shmarov

I. L. Tutykhina

I. B. Esmagambetov

I. Yu. Gribova

A. S. Bandelyuk

B. S. Naroditsky

A. L. Gintsburg

References

Supplementary files

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