Email this article A Novel High-resolving Method for Genomic PCR-fingerprinting of Enterobacteria
- Authors: Isaeva AS1, Kulikov EE1, Tarasyan KK1, Letarov AV1
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Affiliations:
- Winogradsky Institute of Microbiology, Russian Academy of Sciences
- Issue: Vol 2, No 1 (2010)
- Pages: 82-87
- Section: Articles
- Submitted: 17.01.2020
- Published: 15.03.2010
- URL: https://actanaturae.ru/2075-8251/article/view/10767
- DOI: https://doi.org/10.32607/20758251-2010-2-1-82-87
- ID: 10767
Cite item
Abstract
We developed a novel PCR-fingerprinting system for differentiation of enterobacterial strains using a single oligonucleotide primer IS1tr that matches the inverted terminal repeats of the IS1 insertion element. Compared to widely used BOX-PCR and ribotyping methods, our system features higher resolution allowing differentiation of closely related isolates that appear identical in BOX-PCR and ribotyping but differ in their phage sensitivity. The IS1-profiling system is less sensitive to the quality of the material and equipment used. At the same time, BOX-PCR is more universal and suitable for bacterial strain grouping and reconstruction of the low-distance phylogeny. Thus, our system represents an important supplement to the existing set of tools for bacterial strain differentiation; it is particularly valuable for a detailed investigation of highly divergent and rapidly evolving natural bacterial populations and for studies on coliphage ecology. However, some isolates could not be reliably differentiated by IS1-PCR, because of the low number of bands in their patterns. For improvement of IS1-fingerprinting characteristics, we offer to modify the system by introducing the second primer TR8834 hybridizing to the sequence of a transposase gene that is widely spread in enterobacterial genomes.
Full Text
A Novel High-resolving Method for Genomic PCR-fingerprinting of Enterobacteria×
About the authors
A S Isaeva
Winogradsky Institute of Microbiology, Russian Academy of Sciences
E E Kulikov
Winogradsky Institute of Microbiology, Russian Academy of Sciences
K K Tarasyan
Winogradsky Institute of Microbiology, Russian Academy of Sciences
A V Letarov
Winogradsky Institute of Microbiology, Russian Academy of Sciences
Email: letarov@gmail.com
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