Recombinant Human Butyrylcholinesterase As a New-Age Bioscavenger Drug: Development of the Expression System

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Abstract

Butyrylcholinesterase (BChE) is a serine hydrolase (EC 3.1.1.8) which can be found in most animal tissues. This enzyme has a broad spectrum of efficacy against organophosphorus compounds, which makes it a prime candidate for the role of stoichiometric bioscavenger. Development of a new-age DNA-encoded bioscavenger is a vival task. Several transgenic expression systems of human BChE were developed over the past 20 years; however, none of them has been shown to make economic sense or has been approved for administration to humans. In this study, a CHO-based expression system was redesigned, resulting in a significant increase in the production level of functional recombinant human butyrylcholinesterase as compared to the hitherto existing systems. The recombinant enzyme was characterized with Elman and ELISA methods.

About the authors

D. G. Ilyushin

Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry

Author for correspondence.
Email: IlyushinDenis@gmail.com
Russian Federation

O. M. Haertley

Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry

Email: IlyushinDenis@gmail.com
Russian Federation

T. V. Bobik

Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry

Email: IlyushinDenis@gmail.com
Russian Federation

O. G. Shamborant

Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry

Email: IlyushinDenis@gmail.com
Russian Federation

E. A. Surina

Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry

Email: IlyushinDenis@gmail.com
Russian Federation

V. D. Knorre

Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry

Email: IlyushinDenis@gmail.com
Russian Federation

P. Masson

Département de Toxilogie, Centre de Recherches du Servise de Sante des Armées

Email: IlyushinDenis@gmail.com
France

I. V. Smirnov

Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry

Email: IlyushinDenis@gmail.com
Russian Federation

A. G. Gabibov

Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry

Email: IlyushinDenis@gmail.com
Russian Federation

N. A. Ponomarenko

Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry

Email: IlyushinDenis@gmail.com
Russian Federation

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Copyright (c) 2013 Ilyushin D.G., Haertley O.M., Bobik T.V., Shamborant O.G., Surina E.A., Knorre V.D., Masson P., Smirnov I.V., Gabibov A.G., Ponomarenko N.A.

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