Depolarization-Induced Calcium-Independent Synaptic Vesicle Exo- and Endocytosis at Frog Motor Nerve Terminals

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Abstract

The transmitter release and synaptic vesicle exo- and endocytosis induced by constant current depolarization of nerve terminals were studied by microelectode extracellular recording of miniature endplate currents and fluorescent microscopy (FM 1-43 styryl dye). Depolarization of the plasma membrane of nerve terminals in the control specimen was shown to significantly increase the MEPC frequency (quantal transmitter release) and exocytotic rate (FM 1-43 unloading from the synaptic vesicles preliminarily stained with the dye), which was caused by a rise in the intracellular Ca 2+ concentration due to opening of voltage-gated Ca channels. A slight increase in the MEPC frequency and in the rate of synaptic vesicle exocytosis was observed under depolarization in case of blockade of Ca channels and chelating of intracellular Ca 2+ ions (cooperative action of Cd 2+ and EGTA-AM). The processes of synaptic vesicle endocytosis (FM 1-43 loading) were proportional to the number of synaptic vesicles that had undergone exocytosis both in the control and in case of cooperative action of Cd 2+ and EGTA-AM. A hypothesis has been put forward that Ca-independent synaptic vesicle exo- and endocytosis that can be induced directly by depolarization of the membrane exists in the frog motor terminal in addition to the conventional Ca-dependent process.

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Depolarization-Induced Calcium-Independent Synaptic Vesicle Exo- and Endocytosis at Frog Motor Nerve Terminals

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M. M. Abdrakhmanov

Kazan State Medical University

Author for correspondence.
Email: fysio@rambler.ru
Россия

A. M. Petrov

Kazan State Medical University

Email: fysio@rambler.ru
Россия

P. N. Grigoryev

Kazan State Medical University

Email: fysio@rambler.ru
Россия

A. L. Zefirov

Kazan State Medical University

Email: fysio@rambler.ru
Россия

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Copyright (c) 2013 Abdrakhmanov M.M., Petrov A.M., Grigoryev P.N., Zefirov A.L.

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