Protective Immune Response against Bacillus anthracis Induced by Intranasal Introduction of a Recombinant Adenovirus Expressing the Protective Antigen Fused to the Fc-fragment of IgG2a

D. N. Shcherbinin; Щербинин Д. Н.; I. B. Esmagambetov; Есмагамбетов И. Б.; A. N. Noskov; Носков А. Н.; Yu. O. Selyaninov; Селянинов Ю. О.; I. L. Tutykhina; Тутыхина И. Л.; M. M. Shmarov; Шмаров М. М.; D. Yu. Logunov; Логунов Д. Ю.; B. S. Naroditskiy; Народицкий Б. С.; A. L. Gintsburg; Гинцбург А. Л.

doi:10.32607/20758251-2014-6-1-76-84

Protective Immune Response against Bacillus anthracis Induced by Intranasal Introduction of a Recombinant Adenovirus Expressing the Protective Antigen Fused to the Fc-fragment of IgG2a

Authors: Shcherbinin D.N.¹, Esmagambetov I.B.¹, Noskov A.N.¹, Selyaninov Y.O.², Tutykhina I.L.¹, Shmarov M.M.¹, Logunov D.Y.¹, Naroditskiy B.S.¹, Gintsburg A.L.¹
Affiliations:
1. Gamaleya Research Institute for Epidemiology and Microbiology, Ministry of Public Health of the Russian Federation
2. National Research Institute for Veterinary Virology and Microbiology of Russia
Issue: Vol 6, No 1 (2014)
Pages: 76-84
Section: Research Articles
Submitted: 17.01.2020
Published: 15.03.2014
URL: https://actanaturae.ru/2075-8251/article/view/10566
DOI: https://doi.org/10.32607/20758251-2014-6-1-76-84
ID: 10566

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Abstract

Anthrax is a particularly dangerous infectious disease that affects humans and livestock. It is characterized by intoxication, serosanguineous skin lesions, development of lymph nodes and internal organs, and may manifest itsself in either a cutaneous or septic form. The pathogenic agent is Bacillus anthracis, a grampositive, endospore-forming, rod-shaped aerobic bacterium. Efficacious vaccines that can rapidly induce a long-term immune response are required to prevent anthrax infection in humans. In this study, we designed three recombinant human adenovirus serotype-5-based vectors containing various modifications of the fourth domain of the B. anthracis protective antigen (PA). Three PA modifications were constructed: a secretable form (Ad-sPA), a non-secretable form (Ad-cPA), and a form with the protective antigen fused to the Fc fragment of immunoglobulin G2a (Ad-PA-Fc). All these forms exhibited protective properties against Bacillus anthracis. The highest level of protection was induced by the Ad-PA-Fc recombinant adenovirus. Our findings indicate that the introduction of the Fc antibody fragment into the protective antigen significantly improves the protective properties of the Ad-PA-Fc adenovirus against B. anthracis.

Keywords

Bacillus anthracis, immunization, protective antigen, recombinant adenovirus

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Protective Immune Response against Bacillus anthracis Induced by Intranasal Introduction of a Recombinant Adenovirus Expressing the Protective Antigen Fused to the Fc-fragment of IgG2a

Full Text

Abstract

Keywords

About the authors

D. N. Shcherbinin

I. B. Esmagambetov

A. N. Noskov

Yu. O. Selyaninov

I. L. Tutykhina

M. M. Shmarov

D. Yu. Logunov

B. S. Naroditskiy

A. L. Gintsburg

References

Supplementary files

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