Protective Immune Response against Bacillus anthracis Induced by Intranasal Introduction of a Recombinant Adenovirus Expressing the Protective Antigen Fused to the Fc-fragment of IgG2a

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Abstract

Anthrax is a particularly dangerous infectious disease that affects humans and livestock. It is characterized by intoxication, serosanguineous skin lesions, development of lymph nodes and internal organs, and may manifest itsself in either a cutaneous or septic form. The pathogenic agent is Bacillus anthracis, a grampositive, endospore-forming, rod-shaped aerobic bacterium. Efficacious vaccines that can rapidly induce a long-term immune response are required to prevent anthrax infection in humans. In this study, we designed three recombinant human adenovirus serotype-5-based vectors containing various modifications of the fourth domain of the B. anthracis protective antigen (PA). Three PA modifications were constructed: a secretable form (Ad-sPA), a non-secretable form (Ad-cPA), and a form with the protective antigen fused to the Fc fragment of immunoglobulin G2a (Ad-PA-Fc). All these forms exhibited protective properties against Bacillus anthracis. The highest level of protection was induced by the Ad-PA-Fc recombinant adenovirus. Our findings indicate that the introduction of the Fc antibody fragment into the protective antigen significantly improves the protective properties of the Ad-PA-Fc adenovirus against B. anthracis.

About the authors

D. N. Shcherbinin

Gamaleya Research Institute for Epidemiology and Microbiology, Ministry of Public Health of the Russian Federation

Author for correspondence.
Email: dim284@inbox.ru
Россия

I. B. Esmagambetov

Gamaleya Research Institute for Epidemiology and Microbiology, Ministry of Public Health of the Russian Federation

Email: dim284@inbox.ru
Россия

A. N. Noskov

Gamaleya Research Institute for Epidemiology and Microbiology, Ministry of Public Health of the Russian Federation

Email: dim284@inbox.ru
Россия

Yu. O. Selyaninov

National Research Institute for Veterinary Virology and Microbiology of Russia

Email: dim284@inbox.ru
Россия

I. L. Tutykhina

Gamaleya Research Institute for Epidemiology and Microbiology, Ministry of Public Health of the Russian Federation

Email: dim284@inbox.ru
Россия

M. M. Shmarov

Gamaleya Research Institute for Epidemiology and Microbiology, Ministry of Public Health of the Russian Federation

Email: dim284@inbox.ru
Россия

D. Yu. Logunov

Gamaleya Research Institute for Epidemiology and Microbiology, Ministry of Public Health of the Russian Federation

Email: dim284@inbox.ru
Россия

B. S. Naroditskiy

Gamaleya Research Institute for Epidemiology and Microbiology, Ministry of Public Health of the Russian Federation

Email: dim284@inbox.ru
Россия

A. L. Gintsburg

Gamaleya Research Institute for Epidemiology and Microbiology, Ministry of Public Health of the Russian Federation

Email: dim284@inbox.ru
Россия

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Copyright (c) 2014 Shcherbinin D.N., Esmagambetov I.B., Noskov A.N., Selyaninov Y.O., Tutykhina I.L., Shmarov M.M., Logunov D.Y., Naroditskiy B.S., Gintsburg A.L.

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