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Protective Immune Response against Bacillus anthracis Induced by Intranasal Introduction of a Recombinant Adenovirus Expressing the Protective Antigen Fused to the Fc-fragment of IgG2a
- Authors: Shcherbinin D.N.1, Esmagambetov I.B.1, Noskov A.N.1, Selyaninov Y.O.2, Tutykhina I.L.1, Shmarov M.M.1, Logunov D.Y.1, Naroditskiy B.S.1, Gintsburg A.L.1
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Affiliations:
- Gamaleya Research Institute for Epidemiology and Microbiology, Ministry of Public Health of the Russian Federation
- National Research Institute for Veterinary Virology and Microbiology of Russia
- Issue: Vol 6, No 1 (2014)
- Pages: 76-84
- Section: Research Articles
- URL: https://actanaturae.ru/2075-8251/article/view/10566
- DOI: https://doi.org/10.32607/20758251-2014-6-1-76-84
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Abstract
Anthrax is a particularly dangerous infectious disease that affects humans and livestock. It is characterized by intoxication, serosanguineous skin lesions, development of lymph nodes and internal organs, and may manifest itsself in either a cutaneous or septic form. The pathogenic agent is Bacillus anthracis, a grampositive, endospore-forming, rod-shaped aerobic bacterium. Efficacious vaccines that can rapidly induce a long-term immune response are required to prevent anthrax infection in humans. In this study, we designed three recombinant human adenovirus serotype-5-based vectors containing various modifications of the fourth domain of the B. anthracis protective antigen (PA). Three PA modifications were constructed: a secretable form (Ad-sPA), a non-secretable form (Ad-cPA), and a form with the protective antigen fused to the Fc fragment of immunoglobulin G2a (Ad-PA-Fc). All these forms exhibited protective properties against Bacillus anthracis. The highest level of protection was induced by the Ad-PA-Fc recombinant adenovirus. Our findings indicate that the introduction of the Fc antibody fragment into the protective antigen significantly improves the protective properties of the Ad-PA-Fc adenovirus against B. anthracis.
About the authors
D. N. Shcherbinin
Gamaleya Research Institute for Epidemiology and Microbiology, Ministry of Public Health of the Russian Federation
Author for correspondence.
Email: dim284@inbox.ru
Russian Federation
I. B. Esmagambetov
Gamaleya Research Institute for Epidemiology and Microbiology, Ministry of Public Health of the Russian Federation
Email: dim284@inbox.ru
Russian Federation
A. N. Noskov
Gamaleya Research Institute for Epidemiology and Microbiology, Ministry of Public Health of the Russian Federation
Email: dim284@inbox.ru
Russian Federation
Yu. O. Selyaninov
National Research Institute for Veterinary Virology and Microbiology of Russia
Email: dim284@inbox.ru
Russian Federation
I. L. Tutykhina
Gamaleya Research Institute for Epidemiology and Microbiology, Ministry of Public Health of the Russian Federation
Email: dim284@inbox.ru
Russian Federation
M. M. Shmarov
Gamaleya Research Institute for Epidemiology and Microbiology, Ministry of Public Health of the Russian Federation
Email: dim284@inbox.ru
Russian Federation
D. Yu. Logunov
Gamaleya Research Institute for Epidemiology and Microbiology, Ministry of Public Health of the Russian Federation
Email: dim284@inbox.ru
Russian Federation
B. S. Naroditskiy
Gamaleya Research Institute for Epidemiology and Microbiology, Ministry of Public Health of the Russian Federation
Email: dim284@inbox.ru
Russian Federation
A. L. Gintsburg
Gamaleya Research Institute for Epidemiology and Microbiology, Ministry of Public Health of the Russian Federation
Email: dim284@inbox.ru
Russian Federation
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