Email this article Protective Immune Response against Bacillus anthracis Induced by Intranasal Introduction of a Recombinant Adenovirus Expressing the Protective Antigen Fused to the Fc-fragment of IgG2a
- Authors: Shcherbinin D.N.1, Esmagambetov I.B.1, Noskov A.N.1, Selyaninov Y.O.2, Tutykhina I.L.1, Shmarov M.M.1, Logunov D.Y.1, Naroditskiy B.S.1, Gintsburg A.L.1
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Affiliations:
- Gamaleya Research Institute for Epidemiology and Microbiology, Ministry of Public Health of the Russian Federation
- National Research Institute for Veterinary Virology and Microbiology of Russia
- Issue: Vol 6, No 1 (2014)
- Pages: 76-84
- Section: Research Articles
- Submitted: 17.01.2020
- Published: 15.03.2014
- URL: https://actanaturae.ru/2075-8251/article/view/10566
- DOI: https://doi.org/10.32607/20758251-2014-6-1-76-84
- ID: 10566
Cite item
Abstract
Anthrax is a particularly dangerous infectious disease that affects humans and livestock. It is characterized by intoxication, serosanguineous skin lesions, development of lymph nodes and internal organs, and may manifest itsself in either a cutaneous or septic form. The pathogenic agent is Bacillus anthracis, a grampositive, endospore-forming, rod-shaped aerobic bacterium. Efficacious vaccines that can rapidly induce a long-term immune response are required to prevent anthrax infection in humans. In this study, we designed three recombinant human adenovirus serotype-5-based vectors containing various modifications of the fourth domain of the B. anthracis protective antigen (PA). Three PA modifications were constructed: a secretable form (Ad-sPA), a non-secretable form (Ad-cPA), and a form with the protective antigen fused to the Fc fragment of immunoglobulin G2a (Ad-PA-Fc). All these forms exhibited protective properties against Bacillus anthracis. The highest level of protection was induced by the Ad-PA-Fc recombinant adenovirus. Our findings indicate that the introduction of the Fc antibody fragment into the protective antigen significantly improves the protective properties of the Ad-PA-Fc adenovirus against B. anthracis.
About the authors
D. N. Shcherbinin
Gamaleya Research Institute for Epidemiology and Microbiology, Ministry of Public Health of the Russian Federation
Author for correspondence.
Email: dim284@inbox.ru
Россия
I. B. Esmagambetov
Gamaleya Research Institute for Epidemiology and Microbiology, Ministry of Public Health of the Russian Federation
Email: dim284@inbox.ru
Россия
A. N. Noskov
Gamaleya Research Institute for Epidemiology and Microbiology, Ministry of Public Health of the Russian Federation
Email: dim284@inbox.ru
Россия
Yu. O. Selyaninov
National Research Institute for Veterinary Virology and Microbiology of Russia
Email: dim284@inbox.ru
Россия
I. L. Tutykhina
Gamaleya Research Institute for Epidemiology and Microbiology, Ministry of Public Health of the Russian Federation
Email: dim284@inbox.ru
Россия
M. M. Shmarov
Gamaleya Research Institute for Epidemiology and Microbiology, Ministry of Public Health of the Russian Federation
Email: dim284@inbox.ru
Россия
D. Yu. Logunov
Gamaleya Research Institute for Epidemiology and Microbiology, Ministry of Public Health of the Russian Federation
Email: dim284@inbox.ru
Россия
B. S. Naroditskiy
Gamaleya Research Institute for Epidemiology and Microbiology, Ministry of Public Health of the Russian Federation
Email: dim284@inbox.ru
Россия
A. L. Gintsburg
Gamaleya Research Institute for Epidemiology and Microbiology, Ministry of Public Health of the Russian Federation
Email: dim284@inbox.ru
Россия
References
- Guichard A., Nizet V., Bier E. // Microbes Infect. 2012. V. 14. № 2. P. 97-118.
- Brachman P.S. // Ann. N.Y. Acad. Sci. 1980. № 353. P. 83-93.
- Davies J.C.A. // Central African J. Med. 1982. № 28. P. 291-298.
- Davies J.C.A. // Central African J. Med. 1983. № 29. P. 8-12.
- Davies J.C.A. // Central African J. Med. 1985. № 31. P. 176-180.
- Metcalfe N. // Occup. Med. 2004. V. 54. № 7. P. 489-493.
- Meaney-Delman D., Zotti M.E., Rasmussen S.A., Strasser S., Shadomy S., Turcios-Ruiz R.M., Wendel G.D., Treadwell T.A., Jamieson D.J., Gladus M.A., et al. // Obstet. Gynecol. Dec. 2012. V. 120. № 6. P. 1439-1449.
- Ipatenko N.G., Gavrilov V.A., Manichev A.A., Bastarov S.I., Salenko L.S., Yakovleva T.N., Stepanova V.V., Shmorgun B.I., Kiselev Yu.T., Sayitkhulov B.S. // Veterinary. 1995. № 5. С. 27-30.
- Auerbach S., Wright G.G. // J. Immunol. 1955. V. 75. № 2. P. 129-133.
- Broster M.G., Hibbs S.E. // Salisbury Med. Bull. Sp. Suppl. 1990. № 68. P. 91-92.
- Little S.F., Knudson G.B., Neidhardt F.C. // Ann. Meet. Amer. Soc. Microbiol. 1984. P. 46.
- Ward M.K., McGann V.G., Hogge A.L., Huff M.L., Kanode R.G., Roberts E.O. // J. Infect. Dis. 1965. № 115. P. 59-67.
- McConnell M.J., Hanna P.C., Imperiale M.J. // Infect. Immun. 2006. V. 74. № 2. P. 1009-1015.
- Tan Y., Hackett N.R., Boyer J.L., Crystal R.G. // Hum. Gene. Ther. 2003. V. 14. № 17. P. 1673-1682.
- Hashimoto M., Boyer J.L., Hackett N.R., Wilson J.M., Crystal R.G. // Infect. Immun. 2005. V. 73. № 10. P. 6885-6891.
- Yu Y.Z., Li N., Wang W.B., Wang S., Ma Y., Yu W.Y., Sun Z.W. // Vaccine. 2010. V. 28. № 47. P. 7529-7535.
- McConnell M.J., Hanna P.C., Imperiale M.J. // Mol. Ther. 2007. V. 15. № 1. P. 203-210.
- Shcherbinin D. N., Shmarov M.M., Naroditsky B.S., Rubakova E.I., Kondrateva T.K. // Tubercullosis and lung diseases. 2010. V. 87. № 10. P. 50-53.
- Shcherbinin D. N., Tutyhina I.L., Logunov D.U.,Shmarov M.M., Apt A.S., Kondrateva T.K. Naroditsky B.S. //Med. immunol. 2010. V. 13. № 4-5. P. 347.
- Sigalov A.B. // Adv. Exp. Med. Biol. 2008. № 640. P. 121-163.
- Graham D.B., Akilesh H.M., Gmyrek G.B., Piccio L., Gilfillan S., Sim J., Belizaire R., Carrero J.A., Wang Y., Blaufuss G.S., et al. // Blood. 2010. V. 116. № 17. P. 3208-3218.
- Walport M.J. // N. Engl. J. Med. 2001. V. 344. № 14. P. 1058-1066.
- Tutykhina I.L., Logunov D.Y., Shcherbinin D.N., Shmarov M.M., Tukhvatulin A.I., Naroditsky B.S., Gintsburg A.L. // J. Mol. Med. 2011. V. 89. № 4. P. 331-341.
- Zhang J., Jex E., Feng T., Sivko G.S., Baillie L.W., Goldman S., van Kampen K.R., Tang D.C. // Clin. Vaccine Immunol. 2013. V. 20. № 1. P. 1-8.
- Radjainia M., Hyun J.K., Leysath C.E., Leppla S.H., Mitra A.K. // Proc. Natl. Acad. Sci. USA. 2010. V. 107. № 32. P. 14070-14074.
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