High-throughput Method of One-Step DNA Isolation for PCR Diagnostics of Mycobacterium tuberculosis

D. V. Kapustin; Капустин Д. В.; A. I. Prostyakova; Простякова А. И.; Ya. I. Alexeev; Алексеев Я. И.; D. A. Varlamov; Варламов Д. А.; V. P. Zubov; Зубов В. П.; S. K. Zavriev; Завриев С. К.

doi:10.32607/20758251-2014-6-2-48-52

High-throughput Method of One-Step DNA Isolation for PCR Diagnostics of Mycobacterium tuberculosis

Authors: Kapustin D.V.¹, Prostyakova A.I.¹, Alexeev Y.I.²^,3, Varlamov D.A.²^,3, Zubov V.P.¹, Zavriev S.K.¹
Affiliations:
1. M.M. Shemyakin and Yu.A. Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences
2. ZAO SINTOL
3. Russian National Research Institute of Agricultural Biotechnology of the Russian Academy of Agricultural Sciences
Issue: Vol 6, No 2 (2014)
Pages: 48-52
Section: Research Articles
Submitted: 17.01.2020
Published: 15.06.2014
URL: https://actanaturae.ru/2075-8251/article/view/10546
DOI: https://doi.org/10.32607/20758251-2014-6-2-48-52
ID: 10546

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Full Text

Abstract

The efficiency of one-step and multi-step protocols of DNA isolation from lysed sputum samples containing the Mycobacterium tuberculosis complex has been compared. DNA was isolated using spin-cartridges containing a special silica-based sorbent modified with fluoroplast and polyaniline, or using an automated isolation system. One-step isolation using the obtained sorbent has been shown to ensure a significantly lower DNA loss and higher sensitivity in the PCR detection of Mycobacterium tuberculosis as compared to a system based on sorption and desorption of nucleic acids during the isolation.

Keywords

composite sorbents, DNA isolation, PCR diagnostics, Mycobacterium tuberculosis complex, fluoropolymers, polyaniline

References

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High-throughput Method of One-Step DNA Isolation for PCR Diagnostics of Mycobacterium tuberculosis

Full Text

Abstract

Keywords

About the authors

D. V. Kapustin

A. I. Prostyakova

Ya. I. Alexeev

D. A. Varlamov

V. P. Zubov

S. K. Zavriev

References

Supplementary files

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