The Mechanism of Choline-Mediated Inhibition of Acetylcholine Release in Mouse Motor Synapses

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Abstract

The mechanism of action of tonically applied choline, the agonist of α7 nicotinic acetylcholine receptors (nAChRs), to the spontaneous and evoked release of a neurotransmitter in mouse motor synapses in diaphragm neuromuscular preparations using intracellular microelectrode recordings of miniature endplate potentials (MEPPs) and evoked endplate potentials (EPPs) was studied. Exogenous choline was shown to exhibit a presynaptic inhibitory effect on the amplitude and quantal content of EPPs for the activity of neuromuscular junction evoked by single and rhythmic stimuli. This effect was inhibited either by antagonists of α7-nAChRs, such as methyllycaconitine and α-cobratoxin, or by blocking SK-type calcium-activated potassium (K Ca) channels with apamin or blocking intraterminal ryanodine receptors with ryanodine. A hypothesis was put forward that choline in mouse motoneuron nerve terminals can activate presynaptic α7-nAChRs, followed by the release of the stored calcium through ryanodine receptors and activation of SK-type KCa channels, resulting in sustained decay of the quantal content of the evoked neurotransmitter release.

About the authors

A. E. Gaydukov

Lomonosov Moscow State University

Author for correspondence.
Email: gaydukov@gmail.com
Russian Federation

P. O. Bogacheva

Lomonosov Moscow State University

Email: gaydukov@gmail.com
Russian Federation

E. O. Tarasova

Lomonosov Moscow State University

Email: gaydukov@gmail.com
Russian Federation

O. P. Balezina

Lomonosov Moscow State University

Email: gaydukov@gmail.com
Russian Federation

References

  1. Katz B., Miledi R. // J. Physiol. 1973, V.231, №3, P.549-574
  2. Albuquerque E.X., Pereira E.F., Alkondon M., Rogers S.W. // Physiol. Rev. 2009, V.89, №1, P.73-120
  3. Sine S.M. // Physiol. Rev. 2012, V.92, №3, P.1189-1234
  4. Vizi E.S., Somogyi G.T. // Br. J. Pharmacol. 1989, V.97, №1, P.65-70
  5. Tian L., Prior C., Dempster J., Marshall I.G. // J. Physiol. 1994, V.476, №3, P.517-529
  6. Tsuneki H., Kimura I., Dezaki K., Kimura M., Sala C., Fumagalli G. // Neurosci. Lett. 1995, V.196, №1-2, P.13-16
  7. Faria M., Oliveira L., Timoteo M.A., Lobo M.G., Correia-De-Sa P. // Synapse. 2003, V.49, №2, P.77-88
  8. Tian L., Prior C., Dempster J., Marshall I.G. // Br. J. Pharmacol. 1997, V.122, №6, P.1025-1034
  9. Dehkordi O., Haxhiu M.A., Millis R.M., Dennis G.C., Kc P., Jafri A., Khajavi M., Trouth C.O., Zaidi S.I. // Respir. Physiol. Neurobiol. 2004, V.141, №1, P.21-34
  10. Castro N.G., Albuquerque E.X. // Biophys. J. 1995, V.68, №2, P.516-524
  11. Fucile S. // Cell Calcium. 2004, V.35, №1, P.1-8
  12. Uteshev V.V. // Adv. Exp. Med. Biol. 2012, V.740, P.603-638
  13. Gray R., Rajan A.S., Radcliffe K.A., Yakehiro M., Dani J.A. // Nature 1996, V.383, №6602, P.713-716
  14. Sharma G., Vijayaraghavan S. // Neuron. 2003, V.38, №6, P.929-939
  15. Jiang L., Role L.W. // J. Neurophysiol. 2008, V.99, №4, P.1988-1999
  16. Kalappa B.I., Feng L., Kem W.R., Gusev A.G., Uteshev V.V. // Am. J. Physiol. Cell Physiol. 2011, V.301, №2, P.347-361
  17. Prior C., Singh S. // Br. J. Pharmacol. 2000, V.129, №6, P.1067-1074
  18. Balezina O.P., Fedorin V.V., Gaydukov A.Ye. // Bulletin of experimental biology and medicine. 2006, V.142, №1, P.17-21
  19. Alkondon M., Albuquerque E.X. // Eur. J. Pharmacol. 1990, V.191, №3, P.505-506
  20. Servent D., Antil-Delbeke S., Gaillard C., Corringer P.J., Changeux J.P., Menez A. // Eur. J. Pharmacol. 2000, V.393, №1-3, P.197-204
  21. Tsetlin V.I., Hucho F. // FEBS Lett. 2004, V.557, №1-3, P.9-13
  22. McLachlan E.M., Martin A.R. // J. Physiol. 1981, V.311, P.307-324
  23. Yuhas W.A., Fuchs P.A. // J. Comp. Physiol A. 1999, V.185, №5, P.455-462
  24. Griguoli M., Scuri R., Ragozzino D., Cherubini E. // Eur. J. Neurosci. 2009, V.30, №6, P.1011-1022
  25. Adelman J.P., Maylie J., Sah P. // Annu. Rev. Physiol. 2012, V.74, P.245-269
  26. Wright M.C., Potluri S., Wang X., Dentcheva E., Gautam D., Tessler A., Wess J., Rich M.M., Son Y.J. // J. Neurosci. 2009, V.29, №47, P.14942-14955
  27. Ulus I.H., Millington W.R., Buyukuysal R.L., Kiran B.K. // Biochem. Pharmacol. 1988, V.37, №14, P.2747-2755
  28. Fischer V., Both M., Draguhn A., Egorov A.V. // J. Neurochem. 2014, V.129, №5, P.792-805
  29. Minic J., Molgo J., Karlsson E., Krejci E. // Eur. J. Neurosci. 2002, V.15, №3, P.439-448
  30. Santafe M.M., Lanuza M.A., Garcia N., Tomas J. // Eur. J. Neurosci. 2006, V.23, №8, P.2048-2056
  31. Alkondon M., Pereira E.F., Cortes W.S., Maelicke A., Albuquerque E.X. // Eur. J. Neurosci. 1997, V.9, №12, P.2734-2742
  32. Papke R.L., Bencherif M., Lippiello P. // Neurosci. Lett. 1996, V.213, №3, P.201-204
  33. Papke R.L., Porter Papke J.K. // Br. J. Pharmacol. 2002, V.137, №1, P.49-61
  34. Giniatullin R., Nistri A., Yakel J.L. // Trends Neurosci. 2005, V.28, №7, P.371-378
  35. Zhong C., Talmage D.A., Role L.W. // PLoS One. 2013, V.8, №12, P.e82719
  36. Roncarati R., Di Chio M., Sava A., Terstappen G.C., Fumagalli G. // Neuroscience. 2001, V.104, №1, P.253-262
  37. Balezina O.P., Bukiya A.N., Lapteva V.I. // Russian Journal of Physiology. 2005, V.91, №1, P.61-70
  38. Dickinson J.A., Kew J.N., Wonnacott S. // Mol. Pharmacol. 2008, V.74, №2, P.348-359
  39. Shen J.X., Yakel J.L. // Acta Pharmacol. Sin. 2009, V.30, №6, P.673-680
  40. Lioudyno M., Hiel H., Kong J.H., Katz E., Waldman E., Parameshwaran-Iyer S., Glowatzki E., Fuchs P.A. // J. Neurosci. 2004, V.24, №49, P.11160-11164
  41. Descarries L., Gisiger V., Steriade M. // Prog. Neurobiol. 1997, V.53, №5, P.603-625
  42. Jones I.W., Wonnacott S. // J. Neurosci. 2004, V.24, №50, P.11244-11252

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Copyright (c) 2014 Gaydukov A.E., Bogacheva P.O., Tarasova E.O., Balezina O.P.

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