Redistribution of Free- and Cell-Surface-Bound DNA in Blood of Benign and Malignant Prostate Tumor Patients

O. E. Bryzgunova; Брызгунова O. E.; S. N. Tamkovich; Тамкович С. Н.; A. V. Cherepanova; Черепанова A. В.; S. V. Yarmoshchuk; Ярмощук С. В.; V. I. Permyakova; Пермякова В. И.; O. Yu. Anykeeva; Аникеева O. Ю.; P. P. Laktionov; Лактионов П. П.

doi:10.32607/20758251-2015-7-2-115-118

Redistribution of Free- and Cell-Surface-Bound DNA in Blood of Benign and Malignant Prostate Tumor Patients

Authors: Bryzgunova O.E.¹, Tamkovich S.N.¹^,2, Cherepanova A.V.¹, Yarmoshchuk S.V.³, Permyakova V.I.¹, Anykeeva O.Y.³, Laktionov P.P.¹^,3
Affiliations:
1. Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences
2. Novosibirsk State University
3. Meshalkin Novosibirsk State Research Institute of Circulation Pathology
Issue: Vol 7, No 2 (2015)
Pages: 115-118
Section: Research Articles
Submitted: 17.01.2020
Published: 15.06.2015
URL: https://actanaturae.ru/2075-8251/article/view/10512
DOI: https://doi.org/10.32607/20758251-2015-7-2-115-118
ID: 10512

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Abstract

A direct correlation between the concentration of cell-free and cell-surface-bound circulating DNA (cfDNA and csbDNA, respectively) was demonstrated. Based on an inverse correlation between blood plasma DNase activity and the cfDNA concentration, blood DNases are supposed to regulate the cfDNA concentration. However, no correlation was found between the DNase activity in blood plasma and the csbDNA concentration, indicating that blood DNases are not involved in csbDNA dissociation from the cell surface. The possibility of DNA redistribution between cfDNA and csbDNA indicates that the total pool of circulating DNA (cfDNA +csbDNA) should be used for a correct analysis of marker DNA concentrations and data standardization.

Keywords

plasma, circulating DNA, DNase activity, prostate cancer

References

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Redistribution of Free- and Cell-Surface-Bound DNA in Blood of Benign and Malignant Prostate Tumor Patients

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Abstract

Keywords

About the authors

O. E. Bryzgunova

S. N. Tamkovich

A. V. Cherepanova

S. V. Yarmoshchuk

V. I. Permyakova

O. Yu. Anykeeva

P. P. Laktionov

References

Supplementary files

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