Screening of Potential HIV-1 Inhibitors/ Replication Blockers Using Secure Lentiviral in Vitro System

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The development and usage of safe cell systems for testing agents which possess anti-HIV activity is a very important factor in the design of new drugs. We have described in detail a system we designed that is based on lentiviral vectors (Prokofjeva et. al., Antiviral Therapy, in print) for swift and completely safe screening of potential HIV-1 replication inhibitors. The system enables one to test the efficiency of the inhibitory activity of compounds whose action is directed towards either wild-type HIV-1 reverse transcriptase or integrase, or mutant enzymes corresponding to the drug-resistant virus form. Testing results of a number of already known drugs, which correlate well with published data as well as data on newly synthesized compounds, were obtained. Application of this system substantially broadens the possibilities of preclinical anti-HIV drugs testing

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Screening of Potential HIV-1 Inhibitors/ Replication Blockers Using Secure Lentiviral in Vitro System
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Список литературы

  1. Report on the global AIDS epidemic, World Health Organization.
  2. http://www.hivrussia.org/stat/2009/10.shtml
  3. De Clercq E. // Int. J. Antimicrob. Agents. 2009. V. 33. № 4. P. 307-320.
  4. Kuritzkes D.R., Walker B.D. // Fields Virology / Eds Knipe D.M., Howley P.M. Philadelphia: Lippinkot, Williams & Wilkins, 2007.
  5. Hengge U.R., Ruzicka T., Tyring S.K., Stuschke M., Roggendorf M., Schwartz R.A., Seeber S. // Lancet Infect. Dis. 2002. V. 2. № 5. P. 281-292.
  6. Wittkop L., Günthard H.F., de Wolf F., Dunn D., Cozzi-Lepri A., de Luca A., Kücherer C., Obel N., von Wyl V., Masquelier B., et al. // Lancet Infect. Dis. 2011. V. 11. № 5. P. 363-371.
  7. Núñez M. // Hepatology. 2010. V. 52. № 3. P. 1143-1155.
  8. Izzedine H., Harris M., Perazella M.A. // Nat. Rev. Nephrol. 2009. V. 5. № 10. P. 563-573.
  9. Panyutich A.V., Prassolov V.S., Shydlovskaya E.A., Reznikov M.V., Chumakov P.M., Voitenuk N.N. // Hybridoma. 1990. V. 9. № 4. P. 401-406.
  10. Prassolov V.S., Meyer J., Brandenburg G., Hannemann J., Bergemann J., Ostertag W., Stocking C. // Exp. Hematol. 2001. V. 29. № 6. P. 756-765.
  11. Spirin P.V., Vilgelm A.E., Prassolov V.S. // Mol. Biol. (Mosk). 2008. V. 42(5). P. 814-825.
  12. Spirin P.V., Baskaran D., Rubtsov P.M., Zenkova M.A., Vlassov V.V., Chernolovskaya E.L., Prassolov V.S. // Acta Naturae. 2009. V. 2. № 2 (2). P. 86-90.
  13. Spirin P.V., Baskaran D., Orlova N.N., Rulina A.V., Nikitenko N.A., Chernolovskaya E.L., Zenkova M.A., Vlassov V.V., Rubtsov P.M., Chumakov P.M., et al // Mol. Biol. (Mosk). 2010. V. 44(5). P. 776-786.
  14. Kellam P., Larder B. //Antimicrob. Agents Chemother. 1994. V. 38. № 1. P. 23-30.
  15. Walter H., Schmidt B., Korn K., Vandamme A.M., Harrer T., Uberla K. // J. Clin. Virol. 1999. V. 13. № 1-2. P. 71-80.
  16. Jarmy G., Heinkelein M., Weissbrich B., Jassoy C., Rethwilm A. // J. Med. Virol. 2001. V. 64. № 3. P. 223-231.
  17. Garcia-Perez J., Sanchez-Palomino S., Perez-Olmeda M., Fernandez B., Alcami J. // J. Med. Virol. 2007. V. 79. № 2. P. 127-137.
  18. Cheresiz S.V., Grigoryev I.V., Semenova E.A., Pustylnyak V.O., Vlasov V.V., Pokrovsky A.G. // Dokl. Biochem. Biophys. 2010. V. 435. P. 295-298.
  19. Prassolov V.S., Chumakov P.M. Vector pPS-1-neo for gene transfer and expression in cultivated somatic mammalian cells. Inventor’s certificate № 1440036 from 26.06.1987. Application 4268094. (Russian).
  20. Rosenblum L.L., Patton G., Grigg A.R., Frater A. J., Cain D., Erlwein O., Hill C.L., Clarke J.R., McClure M.O. // Antivir. Chem. Chemother. 2001. V. 12. № 2. P. 91-97.
  21. Smith R.A., Gottlieb G.S., Miller A.D. // Retrovirology. 2010. V. 7. P. 70-81.
  22. Bjerke M., Franco M., Johansson M., Balzarini J., Karlsson A. // Biochem. Pharmacol. 2008. V. 75. № 6. P. 1313-1321.
  23. Perez-Bercoff D., Wurtzer S., Compain S., Benech H., Clavel F. // J. Virol. 2007. V. 81. № 9. P. 4540-4550.
  24. Patick A.K., Boritzki T. J., Bloom L.A. // Antimicrob. Agents Chemother. 1997. V. 41. № 10. P. 2159-2164.
  25. Grob P.M., Wu J.C., Cohen K.A., Ingraham R.H., Shih C.K., Hargrave K.D., McTague T.L., Merluzzi V.J. // AIDS Res. Hum. Retroviruses. 1992. V. 8. № 2. P. 145-152.
  26. Witvrouw M., Arranz M.E., Pannecouque C., Declercq R., Jonckheere H., Schmit J.C., Vandamme A.M., Diaz J.A., Ingate S.T., Desmyter J., et al. // Antimicrob. Agents Chemother. 1998. V. 42. № 3. P. 618-623.
  27. Novikov M.S., Ivanova O.N., Ivanov A.V., Ozerov A.A., Valuev-Elliston V.T., Gurskaya G.V., Kochetkov S.N., Pannecouque C., Balzarini J., Seley-Radtke K.L. // Bioorg. Med. Chem. 2011. V. 19. № 19. P. 5794-5802.
  28. Hazuda D.J., Felock P., Witmer M., Wolfe A., Stillmock K., Grobler J.A., Espeseth A., Gabryelski L., Schleif W., Blau C., et al. // Science. 2000. V. 287. № 5453. P. 646-650.
  29. Paprotka T., Venkatachari N.J., Chaipan C., Burdick R., Delviks-Frankenberry K.A., Hu W.S., Pathak V.K. // J. Virol. 2010. V. 84. № 11. P. 5719-5729.
  30. Gröschel B., Höver G., Doerr H.W., Cinatl J., Jr. // Nucleosides Nucleotides Nucleic Acids. 2001. V. 20. № 4-7. P. 487-492.
  31. Paintsil E., Dutschman G.E., Hu R., Grill S.P., Wang C.J., Lam W., Li F.Y., Ghebremichael M., Northrup V., Cheng Y.C. // Antimicrob. Agents Chemother. 2011. V. 55. № 2. P. 895-903.
  32. Summa V., Petrocchi A., Bonelli F., Crescenzi B., Donghi M., Ferrara M., Fiore F., Gardelli C., Gonzalez Paz O., Hazuda D.J., et al. // J. Med. Chem. 2008. V. 51. № 18. P. 5843-5855.
  33. Arion D., Parniak M.A. // Drug Resist. Updat. 1999. V. 2. № 2. P. 91-95.
  34. Isaguliants M.G., Belikov S.V., Starodubova E.S., Gizatullin R.Z., Rollman E., Zuber B., Zuber A.K., Grishchenko O.I., Rytting A.S., Kallander C.F., et al. // AIDS Res. Hum. Retroviruses. 2004. V. 20. № 2. P. 191-201.
  35. Cruchaga C., Ansó E., Rouzaut A., Martínez-Irujo J.J. // J. Biol. Chem. 2006. V. 281. № 38. P. 27744-27752.
  36. Song Y., Chan J.M.W., Tovian Z., Secrest A., Nagy E., Krysiak K., Bergan K., Parniak M.A., Oldfield E. // Bioorg. Med. Chem. 2008. V. 16. P. 8959-8967.
  37. Jacobson M.A., van der Horst C., Causey D.M., Dehlinger M., Hafner R., Mills J. // J. Infect Dis. 1991. V. 163. № 6. P. 1219-1222.

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© Prokofjeva M.M., Spirin P.V., Yanvarev D.V., Ivanov A.V., Novikov M.S., Stepanov O.A., Gottikh M.B., Kochetkov S.N., Fehse B., Stocking C., Prassolov V.S., 2011

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