Acta NaturaeActa NaturaeActa Naturae2075-8251Acta Naturae Ltd1065610.32607/20758251-2011-3-4-55-65ArticlesСтатьиResearch ArticleScreening of Potential HIV-1 Inhibitors/ Replication Blockers Using Secure Lentiviral in Vitro SystemScreening of Potential HIV-1 Inhibitors/ Replication Blockers Using Secure Lentiviral in Vitro SystemProkofjevaM MSpirinP VYanvarevD VIvanovA VNovikovM SStepanovO AGottikhM BKochetkovS NFehseB

Department for Stem Cell Transplantation University Medical Center Hamburg-Eppendorf

StockingCPrassolovV Sprassolov45@mail.ruEngelhardt Institute of Molecular Biology, Russian Academy of SciencesVolgograd State Medical UniversityBelozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State UniversityResearch Department Cell and Gene TherapyHeinrich-Pette-Institute for Experimental Virology and Immunology1512201134VOL 3, NO4 (2011)ТОМ 3, №4 (2011)556517012020Copyright ©; 2011, Prokofjeva M.M., Spirin P.V., Yanvarev D.V., Ivanov A.V., Novikov M.S., Stepanov O.A., Gottikh M.B., Kochetkov S.N., Fehse B., Stocking C., Prassolov V.S.Copyright ©; 2011, Prokofjeva M.M., Spirin P.V., Yanvarev D.V., Ivanov A.V., Novikov M.S., Stepanov O.A., Gottikh M.B., Kochetkov S.N., Fehse B., Stocking C., Prassolov V.S.2011Prokofjeva M.M., Spirin P.V., Yanvarev D.V., Ivanov A.V., Novikov M.S., Stepanov O.A., Gottikh M.B., Kochetkov S.N., Fehse B., Stocking C., Prassolov V.S.Prokofjeva M.M., Spirin P.V., Yanvarev D.V., Ivanov A.V., Novikov M.S., Stepanov O.A., Gottikh M.B., Kochetkov S.N., Fehse B., Stocking C., Prassolov V.S.https://creativecommons.org/licenses/by/4.0https://actanaturae.ru/2075-8251/article/view/10656

The development and usage of safe cell systems for testing agents which possess anti-HIV activity is a very important factor in the design of new drugs. We have described in detail a system we designed that is based on lentiviral vectors (Prokofjeva et. al., Antiviral Therapy, in print) for swift and completely safe screening of potential HIV-1 replication inhibitors. The system enables one to test the efficiency of the inhibitory activity of compounds whose action is directed towards either wild-type HIV-1 reverse transcriptase or integrase, or mutant enzymes corresponding to the drug-resistant virus form. Testing results of a number of already known drugs, which correlate well with published data as well as data on newly synthesized compounds, were obtained. Application of this system substantially broadens the possibilities of preclinical anti-HIV drugs testing

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