Characteristics of Artificial Virus-like Particles Assembled in vitro from Potato Virus X Coat Protein and Foreign Viral RNAs
Authors:
Arkhipenko MV 1 , Petrova EK 1 , Nikitin NA 1 , Protopopova AD 2 ,1 , Dubrovin EV 1 , Yaminskii IV 2 ,1 , Rodionova NP 1 , Karpova OV 1 , Atabekov JG 1 ,3
Affiliations:
Lomonosov Moscow State University
Advanced Technologies Center
Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University
Issue: Vol 3, No 3 (2011) Pages: 40-46 Section:
Articles
Submitted: 17.01.2020
Published: 15.09.2011
URL: https://actanaturae.ru/2075-8251/article/view/10671
DOI: https://doi.org/10.32607/20758251-2011-3-3-40-46
ID: 10671
Cite item
Abstract
Potato virus X (PVX) and some other potexviruses can be reconstituted in vitro from viral coat protein (CP) and RNA. PVX CP is capable of forming viral ribonucleoprotein complexes (vRNP) not only with homologous, but also with foreign RNAs. This paper presents the structure and properties of vRNP assembled in vitro upon incubation of PVX CP and RNAs of various plant and animal viruses belonging to different taxonomic groups. We have shown that the morphology and translational properties of vRNPs containing foreign (heterologous) RNA are identical to those of homological vRNP (PVX RNA - PVX CP). Our data suggest that the assembly of the “mixed” vRNP in vitro could be started at the 5’-proximal region of the RNA, producing a helical structure of vRNPs with foreign nucleic acids. The formation of heterologous vRNP in vitro with PVX CP appears not to require a specific 5’ end RNA nucleotide sequence, and the PVX CP seems to be able to pack foreign genetic material of various sizes and compositions into artificial virus-like particles.
Full Text
Characteristics of Artificial Virus-like Particles Assembled in vitro from Potato Virus X Coat Protein and Foreign Viral RNAs
About the authors
Lomonosov Moscow State University
Biology Department
Lomonosov Moscow State University
Biology Department
Lomonosov Moscow State University
Biology Department
Advanced Technologies Center; Lomonosov Moscow State University
Physical Department
Lomonosov Moscow State University
Physical Department
Advanced Technologies Center; Lomonosov Moscow State University
Physical Department
Lomonosov Moscow State University
Biology Department
Lomonosov Moscow State University
Email: okar@genebee.msu.su Biology Department
Lomonosov Moscow State University; Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University
Biology Department
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