Acta NaturaeActa NaturaeActa Naturae2075-8251Acta Naturae Ltd1067110.32607/20758251-2011-3-3-40-46ArticlesСтатьиResearch ArticleCharacteristics of Artificial Virus-like Particles Assembled in vitro from Potato Virus X Coat Protein and Foreign Viral RNAsCharacteristics of Artificial Virus-like Particles Assembled in vitro from Potato Virus X Coat Protein and Foreign Viral RNAsArkhipenkoM V

Biology Department

PetrovaE K

Biology Department

NikitinN A

Biology Department

ProtopopovaA D

Physical Department

DubrovinE V

Physical Department

YaminskiiI V

Physical Department

RodionovaN P

Biology Department

KarpovaO V

Biology Department

okar@genebee.msu.suAtabekovJ G

Biology Department

Lomonosov Moscow State UniversityAdvanced Technologies CenterLomonosov Moscow State UniversityBelozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University1509201133VOL 3, NO3 (2011)ТОМ 3, №3 (2011)404617012020Copyright ©; 2011, Arkhipenko M.V., Petrova E.K., Nikitin N.A., Protopopova A.D., Dubrovin E.V., Yaminskii I.V., Rodionova N.P., Karpova O.V., Atabekov J.G.Copyright ©; 2011, Arkhipenko M.V., Petrova E.K., Nikitin N.A., Protopopova A.D., Dubrovin E.V., Yaminskii I.V., Rodionova N.P., Karpova O.V., Atabekov J.G.2011Arkhipenko M.V., Petrova E.K., Nikitin N.A., Protopopova A.D., Dubrovin E.V., Yaminskii I.V., Rodionova N.P., Karpova O.V., Atabekov J.G.Arkhipenko M.V., Petrova E.K., Nikitin N.A., Protopopova A.D., Dubrovin E.V., Yaminskii I.V., Rodionova N.P., Karpova O.V., Atabekov J.G.https://creativecommons.org/licenses/by/4.0https://actanaturae.ru/2075-8251/article/view/10671

Potato virus X (PVX) and some other potexviruses can be reconstituted in vitro from viral coat protein (CP) and RNA. PVX CP is capable of forming viral ribonucleoprotein complexes (vRNP) not only with homologous, but also with foreign RNAs. This paper presents the structure and properties of vRNP assembled in vitro upon incubation of PVX CP and RNAs of various plant and animal viruses belonging to different taxonomic groups. We have shown that the morphology and translational properties of vRNPs containing foreign (heterologous) RNA are identical to those of homological vRNP (PVX RNA - PVX CP). Our data suggest that the assembly of the “mixed” vRNP in vitro could be started at the 5’-proximal region of the RNA, producing a helical structure of vRNPs with foreign nucleic acids. The formation of heterologous vRNP in vitro with PVX CP appears not to require a specific 5’ end RNA nucleotide sequence, and the PVX CP seems to be able to pack foreign genetic material of various sizes and compositions into artificial virus-like particles.

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