Email this article Specific Visualization of Tumor Cells Using Upconversion Nanophosphors
- Authors: Grebenik E.A.1, Generalova A.N.1, Nechaev A.V.2, Khaydukov E.V.3, Mironova K.E.1, Stremovskiy O.A.1, Lebedenko E.N.1, Zvyagin A.V.1,4,5, Deyev S.M.1,5
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Affiliations:
- Shemyakin/Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
- Lomonosov Moscow State University
- Institute of Laser and Information Technologies, Russian Academy of Sciences
- Macquarie University
- Lobachevsky Nizhniy Novgorod State University
- Issue: Vol 6, No 4 (2014)
- Pages: 48-53
- Section: Research Articles
- Submitted: 17.01.2020
- Published: 15.12.2014
- URL: https://actanaturae.ru/2075-8251/article/view/10524
- DOI: https://doi.org/10.32607/20758251-2014-6-4-48-53
- ID: 10524
Cite item
Abstract
The development of targeted constructs on the basis of photoluminescent nanoparticles with a high photo- and chemical stability and absorption/emission spectra in the “transparency window” of biological tissues is an important focus area of present-day medical diagnostics. In this work, a targeted two-component construct on the basis of upconversion nanophosphors (UCNPs) and anti-tumor 4D5 scFv was developed for selective labeling of tumor cells overexpressing the HER2 tumor marker characteristic of a number of human malignant tumors. A high affinity barnase : barstar (Bn : Bs) protein pair, which exhibits high stability in a wide range of pH and temperatures, was exploited as a molecular adapter providing self-assembly of the two-component construct. High selectivity for the binding of the two-component 4D5 scFv-Bn : UCNP-Bs construct to human breast adenocarcinoma SK-BR-3 cells overexpressing HER2 was demonstrated. This approach provides an opportunity to produce similar constructs for the visualization of different specific markers in pathogenic tissues, including malignant tumors.
About the authors
E. A. Grebenik
Shemyakin/Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
Author for correspondence.
Email: katya.ivukina@rambler.ru
Россия
A. N. Generalova
Shemyakin/Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
Email: katya.ivukina@rambler.ru
Россия
A. V. Nechaev
Lomonosov Moscow State University
Email: katya.ivukina@rambler.ru
Россия
E. V. Khaydukov
Institute of Laser and Information Technologies, Russian Academy of Sciences
Email: katya.ivukina@rambler.ru
Россия
K. E. Mironova
Shemyakin/Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
Email: katya.ivukina@rambler.ru
Россия
O. A. Stremovskiy
Shemyakin/Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
Email: katya.ivukina@rambler.ru
Россия
E. N. Lebedenko
Shemyakin/Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
Email: katya.ivukina@rambler.ru
Россия
A. V. Zvyagin
Shemyakin/Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences; Macquarie University; Lobachevsky Nizhniy Novgorod State University
Email: katya.ivukina@rambler.ru
Россия
S. M. Deyev
Shemyakin/Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences; Lobachevsky Nizhniy Novgorod State University
Email: katya.ivukina@rambler.ru
Россия
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