Specific Visualization of Tumor Cells Using Upconversion Nanophosphors

E. A. Grebenik; Гребеник Е. А.; A. N. Generalova; Генералова А. Н.; A. V. Nechaev; Нечаев А. В.; E. V. Khaydukov; Хайдуков Е. В.; K. E. Mironova; Миронова К. Е.; O. A. Stremovskiy; Стремовский О. А.; E. N. Lebedenko; Лебеденко Е. Н.; A. V. Zvyagin; Звягин А. В.; S. M. Deyev; Деев С. М.

doi:10.32607/20758251-2014-6-4-48-53

Specific Visualization of Tumor Cells Using Upconversion Nanophosphors

Authors: Grebenik E.A.¹, Generalova A.N.¹, Nechaev A.V.², Khaydukov E.V.³, Mironova K.E.¹, Stremovskiy O.A.¹, Lebedenko E.N.¹, Zvyagin A.V.¹^,4^,5, Deyev S.M.¹^,5
Affiliations:
1. Shemyakin/Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
2. Lomonosov Moscow State University
3. Institute of Laser and Information Technologies, Russian Academy of Sciences
4. Macquarie University
5. Lobachevsky Nizhniy Novgorod State University
Issue: Vol 6, No 4 (2014)
Pages: 48-53
Section: Research Articles
Submitted: 17.01.2020
Published: 15.12.2014
URL: https://actanaturae.ru/2075-8251/article/view/10524
DOI: https://doi.org/10.32607/20758251-2014-6-4-48-53
ID: 10524

Cite item

Full Text

Abstract

The development of targeted constructs on the basis of photoluminescent nanoparticles with a high photo- and chemical stability and absorption/emission spectra in the “transparency window” of biological tissues is an important focus area of present-day medical diagnostics. In this work, a targeted two-component construct on the basis of upconversion nanophosphors (UCNPs) and anti-tumor 4D5 scFv was developed for selective labeling of tumor cells overexpressing the HER2 tumor marker characteristic of a number of human malignant tumors. A high affinity barnase : barstar (Bn : Bs) protein pair, which exhibits high stability in a wide range of pH and temperatures, was exploited as a molecular adapter providing self-assembly of the two-component construct. High selectivity for the binding of the two-component 4D5 scFv-Bn : UCNP-Bs construct to human breast adenocarcinoma SK-BR-3 cells overexpressing HER2 was demonstrated. This approach provides an opportunity to produce similar constructs for the visualization of different specific markers in pathogenic tissues, including malignant tumors.

Keywords

upconversion nanophosphors, biomarker imaging, anti-tumor antibodies, self-assembly, HER2

References

Nikitin M.P., Shipunova V.O., Deyev S.M., Nikitin P.I. // Nat. Nanotechnol. 2014, V.9, P.716-722
Mironova K.E., Proshkina G.M., Ryabova F.V., Stremovskiy O.A., Lukyanov S.A., Petrov R.V., Deyev S.M. // Theranostics. 2013, V.3, №11, P.831-840
Stepanov A.V., Belogurov A.A., Jr. I.O., Ponomarenko N.A., Stremovskiy O.A., Kozlov L.V., Bichucher A.M., Dmitriev S.E., Smirnov I.V., Shamborant O.G., Balabashin D.S. // PLOS One. 2011, V.6, P.e20991
Generalova A.N., Sizova S.V., Zdobnova T.A., Zarifullina M.M., Artemyev M.V., Baranov A.V., Oleinikov V.A., Zubov V.P., Deyev S.M. // Nanomedicine (London). 2011, V.6, P.195-209
Polanovski O.L., Lebedenko E.N., Deyev S.M. // Biochemistry (Moscow). 2012, V.77, №3, P.227-245
Nahta R., Esteva F.J. // Cancer Lett. 2006, V.232, P.123-138
Deyev S.M., Waibel R., Lebedenko E.N., Schubiger A.P., Plückthun A. // Nat. Biotechnol. 2003, V.21, P.1486-1492
Zdobnova T.A., Dorofeev S.G., Tananaev P.N., Vasiliev R.B., Balandin T.G., Edelweiss E.F., Stremovskiy O.A., Balalaeva I.V., Turchin I.V., Lebedenko E.N. // J. Biomed. Opt. 2009, V.14, P.021004
Serebrovskaya E.O., Edelweiss E.F., Stremovskiy O.A., Lukyanov K.A., Chudakov D.M., Deyev S.M. // Proc. Natl. Acad. Sci. USA. 2009, V.106, P.9221-9225
Balandin T.G., Edelweiss E., Andronova N.V., Treshalina E.M., Sapozhnikov A.M., Deyev S.M. // Invest. New Drugs. 2011, V.29, P.22-32
Deyev S.M., Lebedenko E.N. // Acta Naturae. 2009, V.1, №1, P.32-50
Nikitin M.P., Zdobnova T.A., Lukash S.V., Stremovskiy O.A., Deyev S.M. // Proc. Natl. Acad. Sci. USA. 2010, V.107, №13, P.5827-5832
Zdobnova T.A., Stremovskiy O.A., Lebedenko E.N., Deyev S.M. // PLOS One. 2012, V.7, P.e48248
Glinka E.M., Edelweiss E.F., Sapozhnikov A.M., Deyev S.M. // Gene. 2006, V.366, P.97-103
Sreenivasan V.K.A., Ivukina E.A., Deng W., Kelf T.A., Zdobnova T.A., Lukash S.V., Veryugin B.V., Stremovskiy O.A., Zvyagin A.V., Deyev S.M. // J. Mater. Chem. 2011, V.21, P.65-68
Schreiber G., Fersht A.R. // Nat. Struct. Biol. 1996, V.3, №5, P.427-431
Aghayeva U.F., Nikitin M.P., Lukash S.V., Deyev S.M. // ACS Nano. 2013, V.7, №2, P.950-961
Martsev S.P., Tsybovsky Y.I., Stremovsky O.A., Odincov S.G., Balandin T.G., Arosio P., Kravchuk Z.I., Deyev S.M. // Protein Engineering Design Selection. 2004, V.17, P.85-93
Grebenik E.A., Nadort A., Generalova A.N., Nechaev A.V., Sreenivasan V.K., Khaydukov E.V., Semchishen V.A., Popov A.P., Sokolov V.I., Akhmanov A.S. // J. Biomed. Opt. 2013, V.18, P.76004
Xiong L., Chen Z., Tian Q., Cao T., Xu C., Li F. // Anal. Chem. 2009, V.81, №21, P.8687-8694
Dou Q., Idris N.M., Zhang Y. // Biomaterials. 2013, V.34, №6, P.1722-1731
Yi G., Peng Y., Gao Z. // Chem. Mater. 2011, V.23, P.2729-2734
Mai H., Zhang Y., Sun L., Yan C. // J. Phys. Chem. C. 2007, V.111, P.13721-13729
Yazynin S.A., Deyev S.M., Jucovic M., Hartley R.W. // Gene. 1996, V.169, №2, P.131-132
Rushizky G.W., Greco A.E., Hartley R.W., Jr. I.O., Sober H.A. // Biochemistry. 1963, V.2, №4, P.787-793
Zhan Q., Qian J., Liang H., Somesfalean G., Wang D., He S., Zhang Z., Andersson-Engels S. // ACS Nano. 2011, V.5, №5, P.3744-3757
Pellegrino T., Manna L., Kudera S., Liedl T., Koktysh D., Rogach A.L., Keller S., Rädler J., Natile G., Parak W.J. // Nano Lett. 2004, V.4, P.703-707
Chen G., Qiu H., Prasad P.N., Chen X. // Chem. Rev. 2014, V.114, №10, P.5161-5214

Supplementary files

Supplementary Files

Action

1. JATS XML

Download

Username
Password
Remember me

Forgot password?	Register

Username
Password
Remember me

Forgot password?	Register

Specific Visualization of Tumor Cells Using Upconversion Nanophosphors

Full Text

Abstract

Keywords

About the authors

E. A. Grebenik

A. N. Generalova

A. V. Nechaev

E. V. Khaydukov

K. E. Mironova

O. A. Stremovskiy

E. N. Lebedenko

A. V. Zvyagin

S. M. Deyev

References

Supplementary files

This website uses cookies