Methanogenic Community Dynamics during Anaerobic Utilization of Agricultural Wastes

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Abstract

This work is devoted to the investigation of the methanogenic archaea involved in anaerobic digestion of cattle manure and maize straw on the basis of terminal restriction fragment length polymorphism (TRFLP) analysis of archaeal 16S rRNA genes. The biological diversity and dynamics of methanogenic communities leading to anaerobic degradation of agricultural organic wastes with biogas production were evaluated in laboratory-scale digesters. T-RFLP analysis, along with the establishment of archaeal 16S rRNA gene clone libraries, showed that the methanogenic consortium consisted mainly of members of the genera Methanosarcina and Methanoculleus, with a predominance of Methanosarcina spp. throughout the experiment.

About the authors

A. M. Ziganshin

Kazan (Volga Region) Federal University

Author for correspondence.
Email: a.ziganshin06@fulbrightmail.org
Russian Federation

E. E. Ziganshina

Kazan (Volga Region) Federal University

Email: a.ziganshin06@fulbrightmail.org
Russian Federation

S. Kleinsteuber

UFZ-Helmholtz Centre for Environmental Research

Email: a.ziganshin06@fulbrightmail.org
Germany

J. Pröter

German Biomass Research Centre

Email: a.ziganshin06@fulbrightmail.org
Germany

O. N. Ilinskaya

Kazan (Volga Region) Federal University

Email: a.ziganshin06@fulbrightmail.org
Russian Federation

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Copyright (c) 1970 Ziganshin A.M., Ziganshina E.E., Kleinsteuber S., Pröter J., Ilinskaya O.N.

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