This work is devoted to the investigation of the methanogenic archaea involved in anaerobic digestion of cattle manure and maize straw on the basis of terminal restriction fragment length polymorphism (TRFLP) analysis of archaeal 16S rRNA genes. The biological diversity and dynamics of methanogenic communities leading to anaerobic degradation of agricultural organic wastes with biogas production were evaluated in laboratory-scale digesters. T-RFLP analysis, along with the establishment of archaeal 16S rRNA gene clone libraries, showed that the methanogenic consortium consisted mainly of members of the genera Methanosarcina and Methanoculleus, with a predominance of Methanosarcina spp. throughout the experiment.
Antoni D., Zverlov V.V., Schwarz W.H. // Appl. Microbiol. Biotechnol. 2007. V. 77. P. 23-35.
Krause L., Diaz N.N., Edwards R.A., Gartemann K.H., Krömeke H., Neuweger H., Pühler A., Runte K.J., Schlüter A., Stoye J., et al. // J. Biotechnol. 2008. V. 136. P. 91-101.
Ahn H.K., Smith M.C., Kondrad S.L., White J.W. // Appl. Biochem. Biotechnol. 2010. V. 160. P. 965-975.
Bedoya I.D., Arrieta A.A., Cadavid F.J. // Bioresour. Technol. 2009. V. 100. P. 6624-6629.
Goberna M., Insam H., Franke-Whittle I.H. // Appl. Environ. Microbiol. 2009. V. 75. P. 2566-2572.
Weiland P. // Appl. Microbiol. Biotechnol. 2010. V. 85. P. 849-860.
Ziganshin A.M., Schmidt T., Scholwin F., Il’inskaya O.N., Harms H., Kleinsteuber S. // Appl. Microbiol. Biotechnol. 2011. V. 89. P. 2039-2052.
Holm-Nielsen J.B., Seadi T.A., Oleskowicz-Popiel P // Bioresour. Technol. 2009. V. 100. P. 5478-5484.
El-Mashad H.M., Zhang R. // Bioresour. Technol. 2010. V. 101. P. 4021-4028.
Gomez X., Moran A., Cuetos M.J., Sanchez M.E. // J. Power Sources. 2006. V. 157. P. 727-732.
Davidsson A., Lövstedt C., la Cour Jansen J., Gruvberger C., Aspegren H. // Waste Manage. 2008. V. 28. P. 986-992.
Fountoulakis M.S., Petousi I., Manios T. // Waste Managе. 2010. V. 10. P. 1849-1853.
Nayono S.E., Gallert C., Winter J. // Bioresour. Technol. 2010. V. 101. P. 6998-7004.
Demirel B., Scherer P. // Rev. Environ. Sci. Biotechnol. 2008. V. 7. P. 173-190.
O’Reilly J., Lee C., Collins G., Chinalia F., Mahony T., O’Flaherty V. // Water Res. 2009. V. 43. P. 3365-3374.
Kröber M., Bekel T., Diaz N.N., Goesmann A., Jaenicke S., Krause L., Miller D., Runte K.J., Viehöver P., Pühler A., Schlüter A. // J. Biotechnol. 2009. V. 142. P. 38-49.
Lee C., Kim J., Hwang K., O'Flaherty V., Hwang S. // Water Res. 2009. V. 43. P 157-165.
Nettmann E., Bergmann I., Pramschüfer S., Mundt K., Plogsties V., Herrmann C., Klocke M. // Appl. Environ. Microbiol. 2010. V. 76. P. 2540-2548.
Abdo Z., Schüette U.M., Bent S.J., Williams C.J., Forney L.J., Joyce P. // Environ. Microbiol. 2006. V. 8. P. 929-938.
Culman S.W., Bukowski R., Gauch H.G., Cadillo-Quiroz H., Buckley D.H. // BMC Bioinformatics. 2009. V. 10. P. 171-180.
Altschul S.F., Gish W., Miller W., Myers E.W., Lipman D.J. // J. Mol. Biol. 1990. V. 215. P 403-410.
Wang Q., Garrity G.M., Tiedje J.M., Cole J.R. // Appl. Environ. Microbiol. 2007. V. 73. P 5261-5267.
Gerardi M.H. The microbiology of anaerobic digesters. Hoboken: Wiley-Interscience, 2003. 177 p.
Kendall M.M., Boone D.R. // The order Methanosarcinales. The Prokaryotes - a Handbook on the Biology of Bacteria. 3rd ed. / Eds Dworkin M., Falkow S., Rosenberg E., Schleifer K.H., Stackebrandt E. New York: Springer, 2006. P. 244-256.
Karakashev D., Batstone D.J., Angelidaki I. // Appl. Environ. Microbiol. 2005. V. 71. P. 331-338.
Email this article 
