Induction of a Protective Heterosubtypic Immune Response Against the Influenza Virus by Using Recombinant Adenoviral Vectors Expressing Hemagglutinin of the Influenza H5 Virus

Cover Page

Cite item

Full Text

Abstract

Influenza viruses are characterized by a high degree of antigenic variability, which causes the annual emergence of flu epidemics and irregularly timed pandemics caused by viruses with new antigenic and biological traits. Novel approaches to vaccination can help circumvent this problem. One of these new methods incorporates genetic vaccines based on adenoviral vectors. Recombinant adenoviral vectors which contain hemagglutinin-encoding genes from avian H5N1 and H5N2 (Ad-HA5-1 and Ad-HA5-2) influenza viruses were obtained using the AdEasy Adenoviral Vector System (Stratagene). Laboratory mice received a double intranasal vaccination with Ad-HA5-1 and Ad-HA5-2. This study demonstrates that immunization with recombinant adenoviruses bearing the Н5 influenza virus hemagglutinin gene induces a immune response which protect immunized mice from a lethal dose of the H5 influenza virus. Moreover, it also protects the host from a lethal dose of H1 virus, which belongs to the same clade as H5, but does not confer protection from the subtype H3 influenza virus, which belongs to a different clade. Our data allow us to conclude that adenoviral vectors may become a universal platform for obtaining vaccines against seasonal and pandemic strains of the influenza virus.

Full Text

Induction of a Protective Heterosubtypic Immune Response Against the Influenza Virus by Using Recombinant Adenoviral Vectors Expressing Hemagglutinin of the Influenza H5 Virus
×

About the authors

M M Shmarov

Gamaleya Research Institute of Epidemiology and Microbiology, Russian Academy of Medical Sciences

Email: mmshmarov@gmail.com

E S Sedova

Gamaleya Research Institute of Epidemiology and Microbiology, Russian Academy of Medical Sciences

L V Verkhovskaya

Gamaleya Research Institute of Epidemiology and Microbiology, Russian Academy of Medical Sciences

I A Rudneva

Ivanovsky Virology Research Institute, Russian Academy of Medical Sciences

E A Bogacheva

Gamaleya Research Institute of Epidemiology and Microbiology, Russian Academy of Medical Sciences

Yu A Barykova

Gamaleya Research Institute of Epidemiology and Microbiology, Russian Academy of Medical Sciences

D N Shcherbinin

Gamaleya Research Institute of Epidemiology and Microbiology, Russian Academy of Medical Sciences

A A Lysenko

Gamaleya Research Institute of Epidemiology and Microbiology, Russian Academy of Medical Sciences

I L Tutykhina

Gamaleya Research Institute of Epidemiology and Microbiology, Russian Academy of Medical Sciences

D Y Logunov

Gamaleya Research Institute of Epidemiology and Microbiology, Russian Academy of Medical Sciences

Yu A Smirnov

Ivanovsky Virology Research Institute, Russian Academy of Medical Sciences

B S Naroditsky

Gamaleya Research Institute of Epidemiology and Microbiology, Russian Academy of Medical Sciences

A L Gintsburg

Gamaleya Research Institute of Epidemiology and Microbiology, Russian Academy of Medical Sciences

References

  1. Benihoud K., Yeh P., Perricaudet M. // Curr. Opin. Biotechnol. 1999. V. 10(5). P. 440—7.
  2. Bett A.J., Prevec L.A., Graham F.L., et al. // J. Virol. 1983. V. 67. P. 5911—5921.
  3. Hoelscher M.A., Singh N., Garg S. // J. Infect. Dis. 2008. V. 197(8). P. 1185—1188.
  4. Kashyap A.K., Steel J., Oner A.F., et al. // Proc. Natl. Acad. Sci. USA. 2008. V. 22;105(16). P. 5986—91.
  5. Kopecky-Bromberg S.A., Palese P. // Curr. Top. Microbiol. Immunol. 2009. V. 333. P. 243—67.
  6. Lvov D.K. // Sov. Med. Rev. E. Virol. Rev. 1987. V. 2. P. 15—37.
  7. Melidou A., Gioula G., Exindari M., Chatzidimitriou D., Diza-Mataftsi E. // Eurosurveil-lance. 2009. V. 14. Issue 20.
  8. Shinde V., Bridges C.B., Uyeki T.M. // The New England Journal of Medicine. 2009. V. 360;25. P. 2616—2625.
  9. Smirnov Y.A., Lipatov A.S., Van Beek R., et al. // Acta Virol. 2000. V. 44(1). P. 1—8.
  10. Smirnov Y.A., Lipatov A.S., Gitelman A.K., Claas E.C., Osterhaus A.D. // Arch. Virol. 2000. V. 145(8). P. 1733—41.
  11. Srivastava I.K., Margaret A.L. // Ann. Intcen Med. 2003. V. 138. P. 550—559.
  12. Sui J., Hwang W.C., Perez S., et al. // Nat. Struct. Mol. Biol. 2009. V. 16(3). P. 265—73.
  13. Tang D.C., Zhang J., Toro H., Shi Z., Van Kampen K.R. // Expert Rev. Vaccines. 2009. V. 8(4) P. 469—81.
  14. Throsby M., van den Brink E., Jongeneelen M., et al. // PLoS ONE. 2008. V. 3(12). Issue 12. P. 942.
  15. Toro H., Tang D.C., Suarez D.L., Shi Z. // Vaccine. 2008. V. 26(21). P. 2640—2646.
  16. Van Kampen K.R., Shi Z., Gao P., et al. // Vaccine. 2005. V. 23(8). P. 1029—36.
  17. Webster R.G., Bean W.J., German O.T., et al. // Microbiol. Rev. 1992. V. 56. P. 152—179.
  18. Lugovtseva V.Yu., Vasilyeva D.A. // Classification and Nomenclature of Viruses in Vertebrates. Ulyanovsk. 20 02. p 26 8.
  19. Kochergin-Nikitskiy К.С. // Analysis of Gene Interaction during Crosses between a Weakly Pathogenic Avian H5 Influenza Viruses and a Highly Productive Human Influenza Virus. M., Ivanovsky G.F. Scientific Research Institute of Virology, RAMS. 2007.
  20. Lvov D.K., Zaberezhniy A.D., Aliper T.I. // Priroda. 2006. no. 6.
  21. Stepanova L.A., Migunov A.I., Korotkov A.V., Kuznetsov O.K. // Meditsinskiy Akademicheskiy Zhurnal. 2006. V. 6. no. 4. P. 3—16.

Supplementary files

Supplementary Files
Action
1. JATS XML
Download

Copyright (c) 2010 Shmarov M.M., Sedova E.S., Verkhovskaya L.V., Rudneva I.A., Bogacheva E.A., Barykova Y.A., Shcherbinin D.N., Lysenko A.A., Tutykhina I.L., Logunov D.Y., Smirnov Y.A., Naroditsky B.S., Gintsburg A.L.

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.

This website uses cookies

You consent to our cookies if you continue to use our website.

About Cookies