Derivation of Induced Pluripotent Stem Cells from Fetal Human Skin Fibroblasts

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Abstract

The isolation and study of autologous human stem cells remain among the most urgent problems in cell biology and biomedicine to date. Induced pluripotent stem cells can be derived from human somatic cells by the overexpression of a number of genes. In this study we reprogrammed fetal human skin fibroblasts by transduction with retroviral vectors carrying murine Oct4, Sox2, Klf4, and c-Myc cDNAs. As a result, cells with the protein expression and gene transcription pattern characteristic of human embryonic stem cells were derived. These induced pluripotent cells are capable of differentiation in vitro into the ectoderm, mesoderm, and endoderm derivatives.

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Derivation of Induced Pluripotent Stem Cells from Fetal Human Skin Fibroblasts
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About the authors

S P Medvedev

Institute of Cytology and Genetics, Siberian Branch, Russian Academy of Sciences

A A Malakhova

Institute of Cytology and Genetics, Siberian Branch, Russian Academy of Sciences

E V Grigor'eva

Institute of Cytology and Genetics, Siberian Branch, Russian Academy of Sciences

A I Shevchenko

Institute of Cytology and Genetics, Siberian Branch, Russian Academy of Sciences

E V Dementyeva

Institute of Cytology and Genetics, Siberian Branch, Russian Academy of Sciences

I A Sobolev

Institute of Cytology and Genetics, Siberian Branch, Russian Academy of Sciences

I N Lebedev

Research Institute of Medical Genetics, Siberian Branch, Russian Academy of Medical Sciences

A G Shilov

Institute of Cytology and Genetics, Siberian Branch, Russian Academy of Sciences

I F Zhimulev

Institute of Chemical Biology and Fundamental Medicine, Siberian Branch, Russian Academy of Sciences

S M Zakian

Institute of Cytology and Genetics, Siberian Branch, Russian Academy of Sciences; Research Center of Clinical and Experimental Medicine, Siberian Branch, Russian Academy of Medical Sciences

Email: zakian@bionet.nsc.ru

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Copyright (c) 2010 Medvedev S.P., Malakhova A.A., Grigor'eva E.V., Shevchenko A.I., Dementyeva E.V., Sobolev I.A., Lebedev I.N., Shilov A.G., Zhimulev I.F., Zakian S.M.

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