Development of Recombinant Vaccine against A(H1N1) 2009 Influenza Based on Virus-like Nanoparticles Carrying the Extracellular Domain of M2 Protein

Cover Page

Cite item

Full Text

Abstract

The conventional vaccines currently being used to deal with influenza are based on a virus obtained in chicken embryos or its components. The high variability of the major immunogenic surface proteins - hemagglutinin and neuraminidase-require the development of strain-specific vaccines that match the antigenic specificity of a newly emerging virus. Recombinant vaccines based on single viral proteins that could be easily produced in standard expression systems are attractive alternatives to traditional influenza vaccines. We constructed recombinant nanosized virus-like particles based on a nuclear antigen of the hepatitis B virus. These particles expose on the surface the extracellular domain of the M2 protein of the highly pathogenic A(H1N1) 2009 influenza virus. The methods of production of these virus-like particles in Escherichia coli and their purification were developed. Experiments on animals show that M2sHBc particles are highly immunogenic in mice and provide complete protection against the lethal influenza challenge.

Full Text

Development of Recombinant Vaccine against A(H1N1) 2009 Influenza Based on Virus-like Nanoparticles Carrying the Extracellular Domain of M2 Protein
×

About the authors

R Y Kotlyarov

Centre "Bioengineering" Russian Academy of Sciences

V V Kuprianov

Centre "Bioengineering" Russian Academy of Sciences

A I Migunov

Research Institute of Influenza, Russian Academy of Medical Sciences

L A Stepanova

Research Institute of Influenza, Russian Academy of Medical Sciences

L M Tsybalova

Research Institute of Influenza, Russian Academy of Medical Sciences

O I Kiselev

Research Institute of Influenza, Russian Academy of Medical Sciences

N V Ravin

Centre "Bioengineering" Russian Academy of Sciences

Email: nravin@biengi.ac.ru

K G Skryabin

Centre "Bioengineering" Russian Academy of Sciences

References

  1. Nicholson K., Webster R., Hay A. Textbook of Influenza. Oxford: Blackwell Science, 1998.
  2. Webster R., Bean W., Gorman O., Chambers T., Kawaoka Y. // Microbiol. 1992. Rev. 56. P. 152-179.
  3. Webby R., Perez D., Coleman J., et al. // Lancet. 2004. V. 363. P. 1099-1103.
  4. Neirynck S., Deroo T., Saelens X., et al. // Nat. Med. 1999. V. 5. P. 1157-1163.
  5. Schotsaert M., De Filette M., Fiers W., Saelens X. // Expert Rev Vaccines. 2009. V. 8(4). P. 499-508.
  6. Lamb R., Zebedee S., Richardson C. // Cell. 1985. V. 40. P. 627-633.
  7. Pinto H., Holsinger J., Lamb A. // Cell. 1992. V. 69. P. 517-528.
  8. Fiers W., De Filette M., Birkett A., Neirynck S., Min Jou W. // Virus Res. 2004. V. 103. P. 173-176.
  9. Ito T., Gorman, O., Kawaoka Y., Bean W., Webster, R. // J. Virol. 1991. V. 65. P. 5491-5498.
  10. Feng J., Zhang M., Mozdzanowska K., et al. // Virol. J. 2006. V. 3. P. 102.
  11. De Filette M., Min Jou W., Birkett A., et al. // Virology. 2005. V. 337 (1). P. 149-161.
  12. Ionescu R., Przysiecki C., Liang X., et al. // J. Pharm. Sci. 2006. V. 95 (1). P. 70-79.
  13. Bessa J., Schmitz N., Hinton H., et al. // Eur. J. Immunol. 2008. V. 38 (1). P. 114-126.
  14. Denis J., Acosta-Ramirez E., Zhao Y., et al. // Vaccine. 2008. V. 26 (2728) P. 3395-3403.
  15. Meshcheriakova Iu.A., El’darov M.A., Migunov A.I. et al. // Mol. Biol. (Mosk). 2009. V. 43 (4). P. 741-750.
  16. De Filette M., Fiers W., Martens W., et al. // Vaccine. 2006. V. 24 (4446) P. 6597-6601.
  17. Tompkins S., Zhao Z., Lo C., et al. // Emerg. Infect. Dis. 2007. V. 13 (3) P. 426-435.
  18. Grant S., Jessee J., Bloom F., Hanahan D. // Proc. Natl Acad. Sci. USA. 1990. V. 87. P. 4645-4649.
  19. Wynne S., Crowther R., Leslie A. // Mol. Cell. 1999. V. 3. P. 771-780.
  20. Kratz P., Bottcher B., Nassal M. // Proc. Natl. Acad. USA. 1999. V. 96. P. 1915-1920.
  21. Murray K., Shiau A.L. // Biol. Chem. 1999. V. 380. P. 277-283.
  22. Pumpens P., Grens E. // Intervirology. 2001. V. 44. P. 98-114.
  23. Wingfield P., Stahl S., Williams R., Steven A. // Biochemistry. 1995. V. 34. P. 4919- 4932.
  24. Zheng J., Schodel F., Peterson D. // J. Biol. Chem. 1992. V. 267. P. 9422-9429.

Supplementary files

Supplementary Files
Action
1. JATS XML
Download

Copyright (c) 2010 Kotlyarov R.Y., Kuprianov V.V., Migunov A.I., Stepanova L.A., Tsybalova L.M., Kiselev O.I., Ravin N.V., Skryabin K.G.

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.

This website uses cookies

You consent to our cookies if you continue to use our website.

About Cookies