Email this article Cytotoxic and Immunochemical Properties of Viscumin Encapsulated in Polylactide Microparticles
- Authors: Kolotova E.S.1, Egorova S.G.1, Ramonova A.A.1, Bogorodski S.E.2, Popov V.K.2, Agapov I.I.3, Kirpichnikov M.P.1
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Affiliations:
- Lomonosov Moscow State University
- Institute of Laser and Information Technologies, Russian Academy of Sciences
- Shumakov Federal Research Centre of Transplantology and Artificial Organs
- Issue: Vol 4, No 1 (2012)
- Pages: 101-106
- Section: Research Articles
- URL: https://actanaturae.ru/2075-8251/article/view/10642
- DOI: https://doi.org/10.32607/20758251-2012-4-1-101-106
- ID: 10642
Cite item
Abstract
Biodegradable polylactide microparticles with encapsulated cytotoxic protein viscumin were obtained via the ultrasound-assisted supercritical fluid technique. The size of the microparticles was 10-50 μМ, as shown by electron microscopy. The time course of viscumin release from microparticles was studied using an immunoenzyme test system with anti-viscumin monoclonal antibodies. It was found that 99.91% of the cytotoxic protein was incorporated into polymer microparticles. Only 0.08% of the initially encapsulated viscumin was released from the microparticles following incubation for 120 h in a phosphate-buffered saline at neutral pH. Importantly, the method of ultrasonic dry supercritical fluid encapsulation failed to alter both the cytotoxic potency and the immunochemical properties of the encapsulated viscumin. Thus, this procedure can be used to generate biodegradable polylactide microparticles with encapsulated bioactive substances.
Keywords
About the authors
E. S. Kolotova
Lomonosov Moscow State University
Author for correspondence.
Email: ekaterinakolotova@mail.ru
Russian Federation
S. G. Egorova
Lomonosov Moscow State University
Email: ekaterinakolotova@mail.ru
Russian Federation
A. A. Ramonova
Lomonosov Moscow State University
Email: ekaterinakolotova@mail.ru
Russian Federation
S. E. Bogorodski
Institute of Laser and Information Technologies, Russian Academy of Sciences
Email: ekaterinakolotova@mail.ru
Russian Federation
V. K. Popov
Institute of Laser and Information Technologies, Russian Academy of Sciences
Email: ekaterinakolotova@mail.ru
Russian Federation
I. I. Agapov
Shumakov Federal Research Centre of Transplantology and Artificial Organs
Email: ekaterinakolotova@mail.ru
Russian Federation
M. P. Kirpichnikov
Lomonosov Moscow State University
Email: ekaterinakolotova@mail.ru
Russian Federation
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