Conformational Differences between Active Angiotensins and Their Inactive Precursors

O. N. Solopova; Солопова O. Н.; L. P. Pozdnyakova; Позднякова Л. П.; N. E. Varlamov; Варламов Н. E.; M. N. Bokov; Боков M. Н.; E. V. Morozkina; Морозкина E. В.; Т. А. Yagudin; Ягудин Т. А.; P. G. Sveshnikov; Свешников П. Г.

doi:10.32607/20758251-2012-4-1-74-77

Conformational Differences between Active Angiotensins and Their Inactive Precursors

Authors: Solopova O.N.¹, Pozdnyakova L.P.¹, Varlamov N.E.¹, Bokov M.N.¹, Morozkina E.V.², Yagudin Т.А.², Sveshnikov P.G.¹
Affiliations:
1. Russian Research Center for Molecular Diagnostics and Therapy
2. Bach Institute of Biochemistry, Russian Academy of Sciences
Issue: Vol 4, No 1 (2012)
Pages: 74-77
Section: Research Articles
Submitted: 17.01.2020
Published: 15.03.2012
URL: https://actanaturae.ru/2075-8251/article/view/10636
DOI: https://doi.org/10.32607/20758251-2012-4-1-74-77
ID: 10636

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Abstract

The peptide conformation in the context of a protein polypeptide chain is influenced by proximal amino acid residues. However, the mechanisms of this interference remain poorly understood. We studied the conformation of angiotensins 1, 2 and 3, which are produced naturally in a sequential fashion from a precursor protein angiotensinogen and contain an identical peptide core structure. Using the example of angiotensins 1, 2 and 3, it was shown that similar amino acid sequences may have significant conformational differences in various molecules. In order to assess the conformational changes, we developed a panel of high-affinity mouse monoclonal antibodies against angiotensins 1, 2 and 3 and studied their cross-reactivity in indirect and competitive ELISAs. It was found that the conformations of inactive angiotensin1 and the corresponding fragment of angiotensinogen are similar; the same is true for the conformations of active angiotensins 2 and 3, whereas the conformations of homologous fragments in the active and inactive angiotensins differ significantly.

Keywords

peptides, conformation, angiotensins, angiotensinogen, monoclonal antibodies

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Conformational Differences between Active Angiotensins and Their Inactive Precursors

Full Text

Abstract

Keywords

About the authors

O. N. Solopova

L. P. Pozdnyakova

N. E. Varlamov

M. N. Bokov

E. V. Morozkina

Т. А. Yagudin

P. G. Sveshnikov

References

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