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Analogues of Artificial Human Box C/D Small Nucleolar RNA As Regulators of Alternative Splicing of a pre-mRNA Target
- Authors: Stepanov G.A.1, Semenov D.V.1, Kuligina E.V.1, Koval O.A.1, Rabinov I.V.1, Kit Y.Y.2, Richter V.A.1
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Affiliations:
- Institute of Chemical Biology and Fundamental Medicine, Siberian Branch, Russian Academy of Sciences
- Institute of Cell Biology, National Academy of Sciences of Ukraine
- Issue: Vol 4, No 1 (2012)
- Pages: 32-41
- Section: Research Articles
- URL: https://actanaturae.ru/2075-8251/article/view/10631
- DOI: https://doi.org/10.32607/20758251-2012-4-1-32-41
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Abstract
Small nucleolar RNAs (snoRNAs) play a key role in ribosomal RNA (rRNA) biogenesis. Box C/D snoRNAs guide the site-specific 2’-O-ribose methylation of nucleotides in rRNAs and small nuclear RNAs (snRNAs). A number of box C/D snoRNAs and their fragments have recently been reported to regulate post-transcriptional modifications and the alternative splicing of pre-mRNA. Artificial analogues of U24 snoRNAs directed to nucleotides in 28S and 18S rRNAs, as well as pre-mRNAs and mature mRNAs of human heat shock cognate protein (hsc70), were designed and synthesized in this study. It was found that after the transfection of MCF-7 human cells with artificial box C/D RNAs in complex with lipofectamine, snoRNA analogues penetrated into cells and accumulated in the cytoplasm and nucleus. It was demonstrated that the transfection of cultured human cells with artificial box C/D snoRNA targeted to pre-mRNAs induce partial splicing impairments. It was found that transfection with artificial snoRNAs directed to 18S and 28S rRNA nucleotides, significant for ribosome functioning, induce a decrease in MCF-7 cell viability.
About the authors
G. A. Stepanov
Institute of Chemical Biology and Fundamental Medicine, Siberian Branch, Russian Academy of Sciences
Author for correspondence.
Email: stepanovga@niboch.nsc.ru
Russian Federation
D. V. Semenov
Institute of Chemical Biology and Fundamental Medicine, Siberian Branch, Russian Academy of Sciences
Email: stepanovga@niboch.nsc.ru
Russian Federation
E. V. Kuligina
Institute of Chemical Biology and Fundamental Medicine, Siberian Branch, Russian Academy of Sciences
Email: stepanovga@niboch.nsc.ru
Russian Federation
O. A. Koval
Institute of Chemical Biology and Fundamental Medicine, Siberian Branch, Russian Academy of Sciences
Email: stepanovga@niboch.nsc.ru
Russian Federation
I. V. Rabinov
Institute of Chemical Biology and Fundamental Medicine, Siberian Branch, Russian Academy of Sciences
Email: stepanovga@niboch.nsc.ru
Russian Federation
Yu. Ya. Kit
Institute of Cell Biology, National Academy of Sciences of Ukraine
Email: stepanovga@niboch.nsc.ru
Ukraine
V. A. Richter
Institute of Chemical Biology and Fundamental Medicine, Siberian Branch, Russian Academy of Sciences
Email: stepanovga@niboch.nsc.ru
Russian Federation
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