Transfer and Expression of Small Interfering RNAs in Mammalian Cells Using Lentiviral Vectors

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Abstract

RNA interference is a convenient tool for modulating gene expression. The widespread application of RNA interference is made difficult because of the imperfections of the methods used for efficient target cell delivery of whatever genes are under study. One of the most convenient and efficient gene transfer and expression systems is based on the use of lentiviral vectors, which direct the synthesis of small hairpin RNAs (shRNAs), the precursors of siRNAs. The application of these systems enables one to achieve sustainable and long-term shRNA expression in cells. This review considers the adaptation of the processing of artificial shRNA to the mechanisms used by cellular microRNAs and simultaneous expression of several shRNAs as potential approaches for producing lentiviral vectors that direct shRNA synthesis. Approaches to using RNA interference for the treatment of cancer, as well as hereditary and viral diseases, are under active development today. The improvement made to the methods for constructing lentiviral vectors and the investigation into the mechanisms of processing of small interfering RNA allow one to now consider lentiviral vectors that direct shRNA synthesis as one of the most promising tools for delivering small interfering RNAs.

About the authors

T. D. Lebedev

Engelhardt Institute of Molecular Biology, Russian Academy of Sciences

Author for correspondence.
Email: saint_john@list.ru
Россия

P. V. Spirin

Engelhardt Institute of Molecular Biology, Russian Academy of Sciences

Email: saint_john@list.ru
Россия

V. S. Prassolov

Engelhardt Institute of Molecular Biology, Russian Academy of Sciences

Email: saint_john@list.ru
Россия

References

  1. Meister G., Tuschl T. // Nature 2004, V.431, №7006, P.343-349
  2. Tomari Y., Zamore P.D. // Genes Dev. 2005, V.19, №5, P.517-529
  3. Kim K., Lee Y.S., Carthew R.W. // RNA 2007, V.13, №1, P.22-29
  4. Macrae I.J., Ma E., Zhou M., Robinson C.V., Doudna J.A. // Proc. Natl. Acad. Sci. USA. 2008, V.105, №2, P.512-517
  5. Collins R.E., Cheng X. // FEBS Lett. 2005, V.579, №26, P.5841-5849
  6. Shen B., Goodman H.M. // Science 2004, V.306, №5698, P.997
  7. Carthew R.W., Sontheimer E.J. // Cell 2009, V.136, №4, P.642-655
  8. Lippman Z., Martienssen R. // Nature 2004, V.431, №7006, P.364-370
  9. Kim V.N. // Nat. Rev. Mol. Cell Biol. 2005, V.6, №5, P.376-385
  10. Bartel D.P. // Cell 2004, V.116, №2, P.281-297
  11. Han J., Lee Y., Yeom K.H., Nam J.W., Heo I., Rhee J.K., Sohn S.Y., Cho Y., Zhang B.T., Kim V.N. // Cell 2006, V.125, №5, P.887-901
  12. Yi R., Qin Y., Macara I.G., Cullen B.R. // Genes Dev 2003, V.17, №24, P.3011-3016
  13. Gregory R.I., Chendrimada T.P., Cooch N., Shiekhattar R. // Cell 2005, V.123, №4, P.631-640
  14. Lai E.C. // Nat. Genet. 2002, V.30, №4, P.363-364
  15. Wu L., Fan J., Belasco J.G. // Proc. Natl. Acad. Sci. USA 2006, V.103, №11, P.4034-4039
  16. Agrawal N., Dasaradhi P.V., Mohmmed A., Malhotra P., Bhatnagar R.K., Mukherjee S.K. // Microbiol. Mol. Biol. Rev. 2003, V.67, №4, P.657-685
  17. Van den Haute C., Eggermont K., Nuttin B., Debyser Z., Baekelandt V. // Hum. Gene Ther. 2003, V.14, №18, P.1799-1807
  18. Cockrell A.S., Kafri T. // Mol. Biotechnol. 2007, V.36, №3, P.187-204
  19. Spirin P.V., Vil’gelm A.E., Prassolov V.S. // Mol Biol (Mosk). 2008, V.42, №5, P.913-926
  20. Pluta K., Kacprzak M.M. // Acta Biochim. Pol. 2009, V.56, №4, P.531-595
  21. Kotsopoulou E., Kim V.N., Kingsman A.J., Kingsman S.M., Mitrophanous K.A. // J. Virol. 2000, V.74, №10, P.4839-4852
  22. Kappes J.C., Wu X., Wakefield J.K. // Methods Mol. Med. 2003, V.76, P.449-465
  23. Aiken C. // J. Virol. 1997, V.71, №8, P.5871-5877
  24. Croyle M.A., Callahan S.M., Auricchio A., Schumer G., Linse K.D., Wilson J.M., Brunner L.J., Kobinger G.P. // J. Virol. 2004, V.78, №2, P.912-921
  25. Cronin J., Zhang X.Y., Reiser J. // Curr. Gene Ther. 2005, V.5, №4, P.387-398
  26. Bartosch B., Vitelli A., Granier C., Goujon C., Dubuisson J., Pascale S., Scarselli E., Cortese R., Nicosia A., Cosset F.L. // J. Biol. Chem. 2003, V.278, №43, P.41624-41630
  27. Mazarakis N.D., Azzouz M., Rohll J.B., Ellard F.M., Wilkes F.J., Olsen A.L., Carter E.E., Barber R.D., Baban D.F., Kingsman S.M. // Hum. Mol. Genet. 2001, V.10, №19, P.2109-2121
  28. Frecha C., Szécsi J., Cosset F.L., Verhoeyen E. // Current Gene Therapy 2008, V.8, №6, P.449-460
  29. Verhoeyen E., Cosset F.L. // Suppl 1 // The Journal of Gene Medicine 2004, V.6, S1, P.S83-S94
  30. Mátrai J., Chuah M.K., Van den Driessche T. // Molecular Therapy 2010, V.18, №3, P.477-490
  31. Funke S., Maisner A., Mühlebach M.D., Koehl U., Grez M., Cattaneo R., Cichutek K., Buchholz C.J. // Molecular Therapy 2008, V.16, №8, P.1427-1436
  32. Maurice M., Verhoeyen E., Salmon P., Trono D., Russell S.J., Cosset F.L. // Blood. 2002, V.99, №7, P.2342-2350
  33. Verhoeyen E., Dardalhon V., Ducrey-Rundquist O., Trono D., Taylor N., Cosset F.L. // Blood. 2003, V.101, №6, P.2167-2174
  34. Verhoeyen E., Nègre D., Cosset F.L. // Methods in Molecular Biology 2008, V.434, P.99-112
  35. Froelich S., Ziegler L., Stroup K., Wang P. // Biotechnology and Bioengineering 2009, V.104, №1, P.206-215
  36. Lei Y., Joo K.I., Zarzar J., Wong C., Wang P. // Virology Journal 2010, V.7, P.35
  37. Yang L., Bailey L., Baltimore D., Wang P. // Proc. Natl. Acad. Sci. USA. 2006, V.103, №31, P.11479-11484
  38. Yang H., Joo K.I., Ziegler L., Wang P. // Pharm. Res. 2009, V.26, №6, P.1432-1445
  39. Yang L., Yang H., Rideout K., Cho T., Joo K.I., Ziegler L., Elliot A., Walls A., Yu D., Baltimore D. // Nat. Biotechnol. 2008, V.26, №3, P.326-334
  40. Morizono K., Bristol G., Xie Y.M., Kung S.K., Chen I.S. // Virology Journal 2001, V.75, №17, P.8016-8020
  41. Cui Y., Kelleher E., Straley E., Fuchs E., Gorski K., Levitsky H., Borrello I., Civin C.I., Schoenberger S.P., Cheng L. // Nat. Med. 2003, V.9, №7, P.952-958
  42. Gruh I., Wunderlich S., Winkler M., Schwanke K., Heinke J., Blömer U., Ruhparwar A., Rohde B., Li R.K., Haverich A. // The Journal of Gene Medicine 2008, V.10, №1, P.21-32
  43. Dykxhoorn D.M., Novina C.D., Sharp P.A. // Nat. Rev. Mol. Cell. Biol. 2003, V.4, №6, P.457-467
  44. Boden D., Pusch O., Lee F., Tucker L., Shank P.R., Ramratnam B. // Nucl. Acids Res. 2003, V.31, №17, P.5033-5038
  45. Tiscornia G., Singer O., Verma I.M. // Nature Protocols 2006, V.1, №1, P.234-240
  46. Spirin P.V., Baskaran D., Rubtsov P.M., Zenkova M.A., Vlassov V.V., Chernolovskaya E.L., Prassolov V.S. // Acta Naturae 2009, V.1, №2, P.86-90
  47. Spirin P.V., Nikitenko N.A., Lebedev T.D., Rubtsov P.M., Stocking C., Prassolov V.S. // Mol Biol (Mosk). 2011, V.45, №6, P.1036-1045
  48. Grimm D., Streetz K.L., Jopling C.L., Storm T.A., Pandey K., Davis C.R., Marion P., Salazar F., Kay M.A. // Nature 2006, V.411, №7092, P.537-541
  49. Hornung V., Ellegast J., Kim S., Brzozka K., Jung A., Kato H., Poeck H., Akira S., Conzelmann K.K., Schlee M. // Science 2006, V.314, №5801, P.994-997
  50. Pichlmair A., Schulz O., Tan C.P., Naslund T.I., Liljestrom P., Weber F., Reis e Sousa C. // Science 2006, V.314, №5801, P.997-1001
  51. Kenworthy R., Lambert D., Yang F., Wang N., Chen Z., Zhu H., Zhu F., Liu C., Li K., Tang H. // Nucleic Acids Research 2009, V.37, №19, P.6587-6599
  52. Scherer L.J., Frank R., Rossi J.J. // Nucleic Acids Research 2007, V.35, №8, P.2620-2628
  53. Ehsani A., Saetrom P., Zhang J., Alluin J., Li H., Snøve O. Jr., Aagaard L., Rossi J.J. // Molecular Therapy 2010, V.18, №4, P.796-802
  54. An D.S., Qin F.X., Auyeung V.C., Mao S.H., Kung S.K., Baltimore D., Chen I.S. // Molecular Therapy 2006, V.14, №4, P.494-504
  55. Gupta S., Maitra R., Young D., Gupta A., Sen S. // Am. J. Physiol. Heart Circ. Physiol. 2009, V.297, №2, P.627-636
  56. Bot I., Guo J., van Eck M., van Santbrink P.J., Groot P.H., Hildebrand R.B., Seppen J., van Berkel T.J., Biessen E.A. // Blood 2005, V.106, №4, P.1147-1153
  57. Eren-Koçak E., Turner C.A., Watson S.J., Akil H. // Biological Psychiatry 2011, V.69, №6, P.534-540
  58. Mäkinen P.I., Koponen J.K., Kärkkäinen A.M., Malm T.M., Pulkkinen K.H., Koistinaho J., Turunen M.P., Ylä-Herttuala S. // The Journal of Gene Medicine 2006, V.8, №4, P.433-441
  59. Manjunath N., Wu H., Subramanya S., Shankar P. // Adv. Drug Deliv. Rev. 2009, V.61, №9, P.732-745
  60. McBride J.L., Boudreau R.L., Harper S.Q., Staber P.D., Monteys A.M., Martins I., Gilmore B.L., Burstein H., Peluso R.W., Polisky B. // Proc. Natl. Acad. Sci. USA. 2008, V.105, №15, P.5868-5873
  61. Zeng Y., Wagner E.J., Cullen B.R. // Molecular Cell 2002, V.9, №6, P.1327-1333
  62. Amendola M., Passerini L., Pucci F., Gentner B., Bacchetta R., Naldini L. // Molecular Therapy 2009, V.17, №6, P.1039-1052
  63. Chung K.H., Hart C.C., Al-Bassam S., Avery A., Taylor J., Patel P.D., Vojtek A.B., Turner D.L. // Nucleic Acids Research 2006, V.34, №7, P.53
  64. Sano M., Li H., Nakanishi M., Rossi J.J. // Molecular Therapy 2008, V.16, №1, P.170-177
  65. Watanabe T., Sudoh M., Miyagishi M., Akashi H., Arai M., Inoue K., Taira K., Yoshiba M., Kohara M. // Gene Ther 2006, V.13, №11, P.883-892
  66. Weinberg M.S., Ely A., Barichievy S., Crowther C., Mufamadi S., Carmona S., Arbuthnot P. // Molecular Therapy 2007, V.15, №3, P.534-541
  67. Brake O., Hooft K.T., Liu Y.P., Centlivre M., von Eije K.J., Berkhout B. // Molecular Therapy 2008, V.16, №3, P.557-564
  68. Gou D., Weng T., Wang Y., Wang Z., Zhang H., Gao L., Chen Z., Wang P., Liu L. // The Journal of Gene Medicine 2007, V.9, №9, P.751-763
  69. Liu Y.P., Haasnoot J., ter Brake O., Berkhout B., Konstantinova P. // Nucleic Acids Research 2008, V.36, №9, P.2811-2824
  70. Ashfaq U.A., Yousaf M.Z., Aslam M., Ejaz R., Jahan S., Ullah O. // Virology Journal 2011, V.8, P.276
  71. Morris K.V., Rossi J.J. // Gene Ther. 2006, V.13, №6, P.553-558
  72. Zheng Z.M., Tang S., Tao M. // Ann. N.Y. Acad. Sci. 2005, V.158, P.105-118
  73. ter Brake O., Berkhout B. // The Journal of Gene Medicine 2007, V.9, №9, P.743-750
  74. Samson M., Libert F., Doranz B.J., Rucker J., Liesnard C., Farber C.M., Saragosti S., Lapoumeroulie C., Cognaux J., Forceille C. // Nature 1996, V.382, №6593, P.722-725
  75. Anderson J., Akkina R. // Gene Ther. 2007, V.14, №17, P.1287-1297
  76. Qin X.F., An D.S., Chen I.S., Baltimore D. // roc. Natl. Acad. Sci. USA. 2003, V.100, №1, P.183-188
  77. Lee M.T., Coburn G.A., McClure M.O., Cullen B.R. // Virology Journal 2003, V.77, №22, P.11964-11972
  78. Butticaz C., Ciuffi A., Muñoz M., Thomas J., Bridge A., Pebernard S., Iggo R., Meylan P., Telenti A. // Antiviral Therapy 2003, V.8, №5, P.373-377
  79. Brass A.L., Dykxhoorn D.M., Benita Y., Yan N., Engelman A., Xavier R.J., Lieberman J., Elledge S.J. // Science 2008, V.319, №5865, P.921-926
  80. Anderson J., Akkina R. // Retrovirology 2005, V.2, P.53
  81. Banerjea A., Li M.J., Bauer G., Remling L., Lee N.S., Rossi J., Akkina R. // Molecular Therapy 2003, V.8, №1, P.62-71
  82. An D.S., Donahue R.E., Kamata M., Poon B., Metzger M., Mao S.H., Bonifacino A., Krouse A.E., Darlix J.L., Baltimore D. // Proc. Natl. Acad. Sci. USA. 2001, V.104, №32, P.13110-13115
  83. Li M.J., Kim J., Li S., Zaia J., Yee J.K., Anderson J., Akkina R., Rossi J.J. // Molecular Therapy 2005, V.12, №5, P.900-909
  84. Tiemann K., Rossi J.J. // EMBO Mol. Med. 2009, V.1, №3, P.142-151
  85. Anderson J., Li M.J., Palmer B., Remling L., Li S., Yam P., Yee J.K., Rossi J., Zaia J., Akkina R. // Molecular Therapy 2007, V.15, №6, P.1182-1188
  86. Senzer N., Barve M., Kuhn J., Melnyk A., Beitsch P., Lazar M., Lifshitz S., Magee M., Oh J., Mill S.W. // Molecular Therapy 2012, V.20, №3, P.679-686
  87. Zhang B., Pan X., Cobb G.P., Anderson T.A. // Developmental Biology 2007, V.302, №1, P.1-12
  88. Li C., Feng Y., Coukos G., Zhang L. // AAPS J. 2009, V.11, №4, P.747-757
  89. Yu F., Yao H., Zhu P., Zhang X., Pan Q., Gong C., Huang Y., Hu X., Su F., Lieberman J. // Cell 2007, V.131, №6, P.1109-1123
  90. Cimmino A., Calin G.A., Fabbri M., Iorio M.V., Ferracin M., Shimizu M., Wojcik S.E., Aqeilan R.I., Zupo S., Dono M. // Proc. Natl. Acad. Sci. USA. 2005, V.102, №39, P.13944-13949
  91. Kota J., Chivukula R.R., O’Donnell K.A., Wentzel E.A., Montgomery C.L., Hwang H.W., Chang T.C., Vivekanandan P., Torbenson M., Clark K.R. // Cell 2009, V.137, №6, P.1005-1017
  92. Kumar M.S., Erkeland S.J., Pester R.E., Chen C.Y., Ebert M.S., Sharp P.A., Jacks T. // Proc. Natl. Acad. Sci. USA. 2008, V.105, №10, P.3903-3908
  93. DiGiusto D.L., Krishnan A., Li L., Li H., Li S., Rao A., Mi S., Yam P., Stinson S., Kalos M. // Sci. Transl. Med. 2010, V.2, №36, P.36-43
  94. Yoshino S., Hara T., Weng J.S., Takahashi Y., Seiki M., Sakamoto T. // PLoS One 2012, V.7, №4, P.35590
  95. Palchaudhuri R., Hergenrother P.J. // ACS Chem. Biol. 2011, V.6, №1, P.21-33
  96. Mitkevich V.A., Petrushanko I.Y., Spirin P.V., Fedorova T.V., Kretova O.V., Tchurikov N.A., Prassolov V.S., Ilinskaya O.N., Makarov A.A. // Cell Cycle. 2011, V.10, №23, P.4090-4097

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