Email this article Impact of Surface Modification with Gold Nanoparticles on the Bioelectrocatalytic Parameters of Immobilized Bilirubin Oxidase
- Authors: Pankratov D.V.1,2, Zeifman Y.S.2, Dudareva А.V.3, Pankratova G.K.1, Khlupova M.E.1, Parunova Y.M.2, Zajtsev D.N.3, Bashirova N.F.3, Popov V.O.1,2, Shleev S.V.1,2,3
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Affiliations:
- A.N. Bach Institute of Biochemistry of Russian Academy of Sciences
- National Research Center “Kurchatov Institute”
- I.G. Petrovsky Bryansk State University
- Issue: Vol 6, No 1 (2014)
- Pages: 102-106
- Section: Short communications
- Submitted: 17.01.2020
- Published: 15.03.2014
- URL: https://actanaturae.ru/2075-8251/article/view/10571
- DOI: https://doi.org/10.32607/20758251-2014-6-1-102-106
- ID: 10571
Cite item
Abstract
We unveil experimental evidence that put into question the widely held notion concerning the impact of nanoparticles on the bioelectrocatalytic parameters of enzymatic electrodes. Comparative studies of the bioelectrocatalytic properties of fungal bilirubin oxidase from Myrothecium verrucaria adsorbed on gold electrodes, modified with gold nanoparticles of different diameters, clearly indicate that neither the direct electron transfer rate (standard heterogeneous electron transfer rate constants were calculated to be 31±9 s -1) nor the biocatalytic activity of the adsorbed enzyme (bioelectrocatalytic constants were calculated to be 34±11 s -1) depends on the size of the nanoparticles, which had diameters close to or larger than those of the enzyme molecules.
About the authors
D. V. Pankratov
A.N. Bach Institute of Biochemistry of Russian Academy of Sciences; National Research Center “Kurchatov Institute”
Author for correspondence.
Email: shleev@inbi.ras.ru
Russian Federation
Y. S. Zeifman
National Research Center “Kurchatov Institute”
Email: shleev@inbi.ras.ru
Russian Federation
А. V. Dudareva
I.G. Petrovsky Bryansk State University
Email: shleev@inbi.ras.ru
Russian Federation
G. K. Pankratova
A.N. Bach Institute of Biochemistry of Russian Academy of Sciences
Email: shleev@inbi.ras.ru
Russian Federation
M. E. Khlupova
A.N. Bach Institute of Biochemistry of Russian Academy of Sciences
Email: shleev@inbi.ras.ru
Russian Federation
Y. M. Parunova
National Research Center “Kurchatov Institute”
Email: shleev@inbi.ras.ru
Russian Federation
D. N. Zajtsev
I.G. Petrovsky Bryansk State University
Email: shleev@inbi.ras.ru
Russian Federation
N. F. Bashirova
I.G. Petrovsky Bryansk State University
Email: shleev@inbi.ras.ru
Russian Federation
V. O. Popov
A.N. Bach Institute of Biochemistry of Russian Academy of Sciences; National Research Center “Kurchatov Institute”
Email: shleev@inbi.ras.ru
Russian Federation
S. V. Shleev
A.N. Bach Institute of Biochemistry of Russian Academy of Sciences; National Research Center “Kurchatov Institute”; I.G. Petrovsky Bryansk State University
Email: shleev@inbi.ras.ru
Russian Federation
References
- Murata K., Kajiya K., Nakamura N., Ohno H. // Energy & Environmental Science. 2009. V. 2. № 12. P. 1280-1285.
- Dagys M., Haberska K., Shleev S., Arnebrant T., Kulys J., Ruzgas T. // Electrochemistry Communications. 2010. V. 12. № 7. P. 933-935.
- Pankratov D. V., Zeifman Y. S., Morozova O. V., Shumakovich G. P., Vasil’eva I. S., Shleev S., Popov V. O., Yaropolov A. I. // Electroanalysis. 2013. V. 25. № 5. Р. 1143-1149.
- Salaj-Kosla U, Poller S., Schuhmann W., Shleev S., Magner E. // Bioelectrochemistry. 2013. V. 91. Р. 15-20.
- Ressine A., Vaz-Dominguez C., Fernandez V. M., De Lacey A. L., Laurell T., Ruzgas, T., Shleev S. // Biosensors & Bioelectronics. 2010. V. 25. № 5. P. 1001-1007.
- Gutierrez-Sanchez C., Pita M., Vaz-Dominguez C., Shleev S., De Lacey A. L. // Journal of the American Chemical Society. 2012. V. 134. № 41. P. 17212-17220.
- Haiss W., Thanh N. T. K., Aveyard J., Fernig D. G. // Analytical Chemistry. 2007. V. 79. № 11. P. 4215-4221.
- Murata K., Kajiya K., Nukaga M., Suga Y., Watanabe T., Nakamura N., Ohno H. // Electroanalysis. 2010. V. 22. № 2. P. 185-190.
- Trasatti S., Petrii O. A. // Pure and Applied Chemistry. 1991. V. 63. № 5. P. 711-734.
- Wang X., Falk M., Ortiz R., Matsumura H., Bobacka J., Ludwig R., Bergelin M., Gorton L., Shleev S. // Biosensors & Bioelectronics. 2012. V. 31. № 1. P. 219-225.
- Climent V., Zhang J. D., Friis E. P., Østergaard L. H., Ulstrup, J. // The Journal of Physical Chemistry C. 2012. V. 116. № 1. P. 1232-1243.
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