Impact of Surface Modification with Gold Nanoparticles on the Bioelectrocatalytic Parameters of Immobilized Bilirubin Oxidase

D. V. Pankratov; Панкратов Д. В.; Y. S. Zeifman; Зейфман Ю. С.; А. V. Dudareva; Дударева А. В.; G. K. Pankratova; Панкратова Г. К.; M. E. Khlupova; Хлупова М. Е.; Y. M. Parunova; Парунова Ю. М.; D. N. Zajtsev; Зайцев Д. Н.; N. F. Bashirova; Баширова Н. Ф.; V. O. Popov; Попов В. О.; S. V. Shleev; Шлеев С. В.

doi:10.32607/20758251-2014-6-1-102-106

Impact of Surface Modification with Gold Nanoparticles on the Bioelectrocatalytic Parameters of Immobilized Bilirubin Oxidase

Authors: Pankratov D.V.¹^,2, Zeifman Y.S.², Dudareva А.V.³, Pankratova G.K.¹, Khlupova M.E.¹, Parunova Y.M.², Zajtsev D.N.³, Bashirova N.F.³, Popov V.O.¹^,2, Shleev S.V.¹^,2^,3
Affiliations:
1. A.N. Bach Institute of Biochemistry of Russian Academy of Sciences
2. National Research Center “Kurchatov Institute”
3. I.G. Petrovsky Bryansk State University
Issue: Vol 6, No 1 (2014)
Pages: 102-106
Section: Short communications
Submitted: 17.01.2020
Published: 15.03.2014
URL: https://actanaturae.ru/2075-8251/article/view/10571
DOI: https://doi.org/10.32607/20758251-2014-6-1-102-106
ID: 10571

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Full Text

Abstract

We unveil experimental evidence that put into question the widely held notion concerning the impact of nanoparticles on the bioelectrocatalytic parameters of enzymatic electrodes. Comparative studies of the bioelectrocatalytic properties of fungal bilirubin oxidase from Myrothecium verrucaria adsorbed on gold electrodes, modified with gold nanoparticles of different diameters, clearly indicate that neither the direct electron transfer rate (standard heterogeneous electron transfer rate constants were calculated to be 31±9 s -1) nor the biocatalytic activity of the adsorbed enzyme (bioelectrocatalytic constants were calculated to be 34±11 s -1) depends on the size of the nanoparticles, which had diameters close to or larger than those of the enzyme molecules.

Keywords

gold nanoparticle, bilirubin oxidase, direct electron transfer, bioelectrocatalysis

References

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Impact of Surface Modification with Gold Nanoparticles on the Bioelectrocatalytic Parameters of Immobilized Bilirubin Oxidase

Full Text

Abstract

Keywords

About the authors

D. V. Pankratov

Y. S. Zeifman

А. V. Dudareva

G. K. Pankratova

M. E. Khlupova

Y. M. Parunova

D. N. Zajtsev

N. F. Bashirova

V. O. Popov

S. V. Shleev

References

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