Email this article Composite Scaffolds Containing Silk Fibroin, Gelatin, and Hydroxyapatite for Bone Tissue Regeneration and 3D Cell Culturing
- Authors: Moisenovich M.M.1, Arkhipova A.Y.1, Orlova A.A.1, Drutskaya M.S.1, Volkova S.V.1, Zacharov S.E.1, Agapov I.I.2, Kirpichnikov M.P.1
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Affiliations:
- Biological Faculty, Moscow State University
- Shumakov Institute of Transplantology and Artificial Organs, Federal Agency for High-Tech Medical Services
- Issue: Vol 6, No 1 (2014)
- Pages: 96-101
- Section: Research Articles
- URL: https://actanaturae.ru/2075-8251/article/view/10570
- DOI: https://doi.org/10.32607/20758251-2014-6-1-96-101
- ID: 10570
Cite item
Abstract
Three-dimensional (3D) silk fibroin scaffolds were modified with one of the major bone tissue derivatives (nano-hydroxyapatite) and/or a collagen derivative (gelatin). Adhesion and proliferation of mouse embryonic fibroblasts (MEF) within the scaffold were increased after modification with either nano-hydroxyapatite or gelatin. However, a significant increase in MEF adhesion and proliferation was observed when both additives were introduced into the scaffold. Such modified composite scaffolds provide a new and better platform to study wound healing, bone and other tissue regeneration, as well as artificial organ bioengineering. This system can further be applied to establish experimental models to study cell-substrate interactions, cell migration and other complex processes, which may be difficult to address using the conventional two-dimensional culture systems.
About the authors
M. M. Moisenovich
Biological Faculty, Moscow State University
Author for correspondence.
Email: igor_agapov@mail.ru
Russian Federation
A. Yu. Arkhipova
Biological Faculty, Moscow State University
Email: igor_agapov@mail.ru
Russian Federation
A. A. Orlova
Biological Faculty, Moscow State University
Email: igor_agapov@mail.ru
Russian Federation
M. S. Drutskaya
Biological Faculty, Moscow State University
Email: igor_agapov@mail.ru
Russian Federation
S. V. Volkova
Biological Faculty, Moscow State University
Email: igor_agapov@mail.ru
Russian Federation
S. E. Zacharov
Biological Faculty, Moscow State University
Email: igor_agapov@mail.ru
Russian Federation
I. I. Agapov
Shumakov Institute of Transplantology and Artificial Organs, Federal Agency for High-Tech Medical Services
Email: igor_agapov@mail.ru
Russian Federation
M. P. Kirpichnikov
Biological Faculty, Moscow State University
Email: igor_agapov@mail.ru
Academician
Russian FederationReferences
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