Generation of iPS Cells from Human Hair Follice Dermal Papilla Cells

I. A. Muchkaeva; Мучкаева И. А.; E. B. Dashinimaev; Дашинимаев Э. Б.; A. S. Artyuhov; Артюхов А. С.; E. P. Myagkova; Мягкова Е. П.; E. A. Vorotelyak; Воротеляк Е. А.; Y. Y. Yegorov; Егоров Е. Е.; K. S. Vishnyakova; Вишнякова Х. С.; J. E. Kravchenko; Кравченко Ю. Е.; S. P. Chumakov; Чумаков С. П.; V. V. Terskikh; Терских В. В.; A. V. Vasiliev; Васильев А. В.

doi:10.32607/20758251-2014-6-1-45-53

Generation of iPS Cells from Human Hair Follice Dermal Papilla Cells

Authors: Muchkaeva I.A.¹^,2, Dashinimaev E.B.¹^,2, Artyuhov A.S.², Myagkova E.P.¹^,2, Vorotelyak E.A.¹^,2, Yegorov Y.Y.³, Vishnyakova K.S.³, Kravchenko J.E.³^,4, Chumakov S.P.³^,4, Terskikh V.V.¹, Vasiliev A.V.¹
Affiliations:
1. Koltsov Institute of Developmental Biology, Russian Academy of Sciences
2. Pirogov Russian National Research Medical University
3. Engelhardt Institute of Molecular Biology, Russian Academy of Sciences
4. Shemyakin and Ovchinnikov Instituse of Bioorganic Chemistry, Russian Academy of Sciences
Issue: Vol 6, No 1 (2014)
Pages: 45-53
Section: Research Articles
Submitted: 17.01.2020
Published: 15.03.2014
URL: https://actanaturae.ru/2075-8251/article/view/10556
DOI: https://doi.org/10.32607/20758251-2014-6-1-45-53
ID: 10556

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Abstract

Dermal papilla (DP) cells are unique regional stem cells of the skin that induce formation of a hair follicle and its regeneration cycle. DP are multipotent stem cells; therefore we supposed that the efficiency of DPC reprogramming could exceed that of dermal fibroblasts reprogramming. We generated induced pluripotent stem cells from human DP cells using lentiviral transfection with Oct4, Sox2, Klf4, and c-Myc, and cultivation of cells both in a medium supplemented with valproic acid and at a physiological level of oxygen (5%). The efficiency of DP cells reprogramming was ~0.03%, while the efficiency of dermal fibroblast reprogramming under the same conditions was ~0.01%. Therefore, we demonstrated the suitability of DP cells as an alternative source of iPS cells.

Keywords

Hair follicle dermal papilla (DP) cells, induced pluripotent stem (iPS) cells, reprogramming

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Generation of iPS Cells from Human Hair Follice Dermal Papilla Cells

Full Text

Abstract

Keywords

About the authors

I. A. Muchkaeva

E. B. Dashinimaev

A. S. Artyuhov

E. P. Myagkova

E. A. Vorotelyak

Y. Y. Yegorov

K. S. Vishnyakova

J. E. Kravchenko

S. P. Chumakov

V. V. Terskikh

A. V. Vasiliev

References

Supplementary files

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