The Effect of Hydrophobic Monoamines on Acid-Sensing Ion Channels ASIC1B

Cover Page

Cite item

Full Text

Abstract

Acid-sensing ion channels (ASICs) are widely distributed in both the central and peripheral nervous systems of vertebrates. The pharmacology of these receptors remains poorly investigated, while the search for new ASIC modulators is very important. Recently, we found that some monoamines, which are blockers of NMDA receptors, inhibit and/or potentiate acid-sensing ion channels, depending on the subunit composition of the channels. The effect of 9-aminoacridine, IEM-1921, IEM-2117, and memantine both on native receptors and on recombinant ASIC1a, ASIC2a, and ASIC3 homomers was studied. In the present study, we have investigated the effect of these four compounds on homomeric ASIC1b channels. Experiments were performed on recombinant receptors expressed in CHO cells using the whole-cell patch clamp technique. Only two compounds, 9-aminoacridine and memantine, inhibited ASIC1b channels. IEM-1921 and IEM-2117 were inactive even at a 1000 μM concentration. In most aspects, the effect of the compounds on ASIC1b was similar to their effect on ASIC1a. The distinguishing feature of homomeric ASIC1b channels is a steep activation-dependence, indicating cooperative activation by protons. In our experiments, the curve of the concentration dependence of ASIC1b inhibition by 9-aminoacridine also had a slope (Hill coefficient) of 3.8, unlike ASIC1a homomers, for which the Hill coefficient was close to 1. This finding indicates that the inhibitory effect of 9-aminoacridine is associated with changes in the activation properties of acid-sensing ion channels.

About the authors

E. I. Nagaeva

Sechenov Institute of Evolutionary Physiology and Biochemistry, Russian Academy of Sciences

Author for correspondence.
Email: eline00111@gmail.com
Россия

N. N. Potapieva

Sechenov Institute of Evolutionary Physiology and Biochemistry, Russian Academy of Sciences

Email: eline00111@gmail.com
Россия

D. B. Tikhonov

Sechenov Institute of Evolutionary Physiology and Biochemistry, Russian Academy of Sciences

Email: eline00111@gmail.com
Россия

References

  1. Du J., Reznikov L.R., Price M.P., Zha X.M., Lu Y., Moninger T.O., Wemmie J.A., Welsh M.J. // Proc. Natl. Acad. Sci. U. S. A. 2014, V.111, №24, P.8961-8966
  2. Deval E., Gasull X., Noël J., Salinas M., Baron A., Diochot S., Lingueglia E. // Pharmacol. Ther. 2010, V.128, №3, P.549-558
  3. Jasti J., Furukawa H., Gonzales E.B., Gouaux E. // Nature 2007, V.449, №7160, P.316-323
  4. Alvarez de la Rosa D., Zhang P., Shao D., White F., Canessa C.M. // Proc. Natl. Acad. Sci. U. S. A. 2002, V.99, №4, P.2326-2331
  5. Baron A., Waldmann R., Lazdunski M. // J. Physiol. 2002, V.539, №2, P.485-494
  6. Bolshakov K.V., Essin K. V., Buldakova S.L., Dorofeeva N.A., Skatchkov S.N., Eaton M.J., Tikhonov D.B., Magazanik L.G. // Neuroscience. 2002, V.110, №4, P.723-730
  7. García-Añoveros J., Derfler B., Neville-Golden J., Hyman B.T., Corey D.P. // Proc. Natl. Acad. Sci. U. S. A. 1997, V.94, №4, P.1459-1464
  8. Lingueglia E., de Weille J.R., Bassilana F., Heurteaux C., Sakai H., Waldmann R., Lazdunski M. // J. Biol. Chem. 1997, V.272, №47, P.29778-29783
  9. Price M.P., Snyder P.M., Welsh M.J. // J. Biol. Chem. 1996, V.271, №14, P.7879-7882
  10. Wemmie J.A., Askwith C.C., Lamani E., Cassell M.D., Freeman J.H., Welsh M.J. // J. Neurosci. 2003, V.23, №13, P.5496-5502
  11. Price M.P., Lewin G.R., McIlwrath S.L., Cheng C., Xie J., Heppenstall P.A., Stucky C.L., Mannsfeldt A.G., Brennan T.J., Drummond H.A. // Nature 2000, V.407, №6807, P.1007-1011
  12. Wemmie J.A., Price M.P., Welsh M.J. // Trends Neurosci. 2006, V.29, №10, P.578-586
  13. Wemmie J.A., Chen J., Askwith C.C., Hruska-Hageman A.M., Price M.P., Nolan B.C., Yoder P.G., Lamani E., Hoshi T., Freeman J.H. // Neuron. 2002, V.34, №3, P.463-477
  14. Wemmie J.A., Coryell M.W., Askwith C.C., Lamani E., Leonard A.S., Sigmund C.D., Welsh M.J. // Proc. Natl. Acad. Sci. U. S. A. 2004, V.101, №10, P.3621-3626
  15. Kreple C.J., Lu Y., Taugher R.J., Schwager-Gutman A.L., Du J., Stump M., Wang Y., Ghobbeh A., Fan R., Cosme C.V. // Nat. Neurosci. 2014, V.17, №8, P.1083-1091
  16. Ziemann A.E., Allen J.E., Dahdaleh N.S., Drebot I.I., Coryell M.W., Wunsch A.M., Lynch C.M., Faraci F.M., Howard M. A., Welsh M.J. // Cell. 2009, V.139, №5, P.1012-1021
  17. Escoubas P., De Weille J.R., Lecoq A., Diochot S., Waldmann R., Champigny G., Moinier D., Ménez A., Lazdunski M. // J. Biol. Chem. 2000, V.275, №33, P.25116-25121
  18. Diochot S., Baron A., Rash L.D., Deval E., Escoubas P., Scarzello S., Salinas M., Lazdunski M. // EMBO J. 2004, V.23, №7, P.1516-1525
  19. Baron A., Diochot S., Salinas M., Deval E., Noël J., Lingueglia E. // Toxicon. 2013, V.75, P.187-204
  20. Waldmann R., Champigny G., Bassilana F. // Nature 1997, V.386, №6621, P.173-177
  21. Kellenberger S., Schild L. // Physiol. Rev. 2002, V.82, №3, P.735-767
  22. Chen X., Orser B.A., MacDonald J.F. // Eur. J. Pharmacol. 2010, V.648, №1-3, P.15-23
  23. Kuduk S.D., Chang R.K., Wai J.M.C., Di Marco C.N., Cofre V., DiPardo R.M., Cook S.P., Cato M.J., Jovanovska A., Urban M.O. // Bioorg. Med. Chem. Lett. 2009, V.19, №15, P.4059-4063
  24. Yu Y., Chen Z., Li W., Cao H., Feng E., Yu F., Liu H., Jiang H., Xu T. // Neuron. 2010, V.68, P.61-72
  25. Benveniste M., Mayer M.L. // J. Physiol. 1995, V.483, №2, P.367-384
  26. Rogawski M.A., Thurkauf A., Yamaguchi S., Rice K.C., Jacobson A.E., Mattson M.V. // J. Pharmacol. Exp. Ther. 1989, V.249, №3, P.708-712
  27. Bolshakov K. V., Kim K.H., Potapjeva N.N., Gmiro V.E., Tikhonov D.B., Usherwood P.N.R., Mellor I.R., Magazanik L.G. // Neuropharmacology. 2005, V.49, №2, P.144-155
  28. Bormann J. // Eur. J. Pharmacol. 1989, V.166, №3, P.591-592
  29. Barygin O.I., Gmiro V.E., Kim K., Magazanik L.G., Tikhonov D.B. // Neurosci. Lett. 2009, V.451, №1, P.29-33
  30. Bolshakov K.V., Gmiro V.E., Tikhonov D.B., Magazanik L.G. // J. Neurochem. 2003, V.87, №1, P.56-65
  31. Tikhonova T.B., Nagaeva E.I., Barygin O.I., Potapieva N.N., Bolshakov K. V., Tikhonov D.B. // Neuropharmacology. 2014, V.89, P.1-10
  32. Hesselager M., Timmermann D.B., Ahring P.K. // J. Biol. Chem. 2004, V.279, №12, P.11006-11015
  33. Chen H.S.V., Pellegrini J.W., Aggarwal S.K., Lei S.Z., Warach S., Jensen F.E., Lipton S.A. // J. Neurosci. 1992, V.12, №11, P.4427-4436

Supplementary files

Supplementary Files
Action
1. JATS XML
Download

Copyright (c) 2015 Nagaeva E.I., Potapieva N.N., Tikhonov D.B.

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.

This website uses cookies

You consent to our cookies if you continue to use our website.

About Cookies