NeuN As a Neuronal Nuclear Antigen and Neuron Differentiation Marker

Cover Page

Cite item

Abstract

The NeuN protein is localized in nuclei and perinuclear cytoplasm of most of the neurons in the central nervous system of mammals. Monoclonal antibodies to the NeuN protein have been actively used in the immunohistochemical research of neuronal differentiation to assess the functional state of neurons in norm and pathology for more than 20 years. Recently, NeuN antibodies have begun to be applied in the differential morphological diagnosis of cancer. However, the structure of the protein, which can be revealed by antibodies to NeuN, remained unknown until recently, and the functions of the protein are still not fully clear. In the present mini-review, data on NeuN accumulated so far are summarized and analyzed. Data on the structure and properties of the protein, its isoforms, intracellular localization, and hypothesized functions are reported. The application field of immunocytochemical detection of NeuN in scientific and clinical studies, as well as the difficulties in the interpretation of the obtained experimental data and their possible causes, is described in details.

About the authors

V. V. Gusel’nikova

Federal State Budgetary Scientific Institution “Institute of Experimental Medicine

Author for correspondence.
Email: Valeriia@yandex.ru
Россия

D. E. Korzhevskiy

Federal State Budgetary Scientific Institution “Institute of Experimental Medicine

Email: Guselnicova.Valeriia@yandex.ru
Россия

References

  1. Ugrumov M.V. // J Chem Neuroanat. 2009, V.38, №4, P.241-256
  2. Ugrumov M., Taxi J., Pronina T., Kurina A., Sorokin A., Sapronova A., Calas A. // Neuroscience. 2014, V.277, P.45-54
  3. Korzhevskii D. E., Grigoriev I. P., Kirik O. V., Sukhorukova E. G., Alekseyeva O. S. // Journal of Evolutionary Biochemistry and Physiology. 2014, V.50, №2, P.177-180
  4. Korzhevskii D.E., Petrova E.S., Kirik O.V., Otellin V.A. // Neurosci Behav Physiol. 2009, V.39, №6, P.513-516
  5. Petrova E. S., Isaeva E. N., Korzhevskii D. E. // Neuroscience and Behavioral Physiology. 2014, V.44, №4, P.478-481
  6. Petrova E.S., Isaeva E.N., Korzhevskii D.E. // Bull of Exp Biol Med. 2014, V.158, №1, P.123-126
  7. Verdiev B.I., Poltavtseva R.A., Podgornyi O.V., Marei M.V., Zinovyeva R.D., Sukhikh G.T., Aleksandrova M.A. // Bull Exp Biol Med. 2009, V.148, №4, P.697-704
  8. Chan M.H., Kleinschmidt-Demasters B.K., Donson A.M., Birks D.K., Foreman N.K., Rush S.Z. // Pediatr Blood Cancer. 2012, V.59, №7, P.1173-1179
  9. You H., Kim Y.I., Im S.Y., Suh-Kim H., Paek S.H., Park S.H., Kim D.G., Jung H.W. // J Neurooncol. 2005, V.74, №1, P.1-8
  10. Hagel C., Treszl A., Fehlert J., Harder J., von Haxthausen F., Kern M., von Bueren A.O., Kordes U. // J Neurooncol. 2013, V.112, №2, P.191-197
  11. Mullen R.J., Buck C.R., Smith A.M. // Development. 1992, V.116, P.201-211
  12. Cannon J.R., Greenamyre J.T. // Neurosci. Lett. 2009, V.464, №1, P.14-17
  13. Sukhorukova E. G. // Neuroscience and Behavioral Physiology. 2014, V.44, №5, P.539-541
  14. Kumar S.S., Buckmaster P.S. // Brain Research. 2007, V.1142, P.54-60
  15. Sarnat H.B., Nochlin D., Born D.E. // Brain Dev. 1998, V.20, P.88-94
  16. Lind D., Franken S., Kappler J., Jankowski J., Schilling K. // J Neurosci Res. 2005, V.79, P.295-302
  17. Dent M.A., Segura-Anaya E., Alva-Medina J., Aranda-Anzaldo A. // FEBS Lett. 2010, V.584, №13, P.2767-2771
  18. Darnell R.B. // Annu Rev Neurosci. 2013, V.36, P.243-270
  19. Weyer A., Schilling K. // J Neurosci Res. 2003, V.73, P.400-409
  20. McPhail L.T., McBride C.B., McGraw J., Steeves J.D., Tetzlaff W. // Exp Neurol. 2004, V.185, P.182-190
  21. Maxeiner S., Glassmann A., Kao H.T., Schilling K. // Histochem Cell Biol. 2014, V.141, P.43-55
  22. Williamson M.P. // Biochem J. 1994, V.297, P.249-260
  23. Kim K.K., Adelstein R.S., Kawamoto S. // J Biol Chem. 2009, V.284, P.31052-31061
  24. Kim K.K., Kim Y.C., Adelstein R.S., Kawamoto S. // Nucleic Acids Res. 2011, V.39, P.3064-3078
  25. Kim K.K., Nam J., Mukouyama Y.S., Kawamoto S. // J Cell Biol. 2013, V.200, P.443-458
  26. Hess D.C., Hill W.D., Martin-Studdard A., Carroll J., Brailer J., Carothers J. // Stroke. 2002, V.33, P.1362-1368
  27. Tanvig M., Blaabjerg M., Andersen R.K., Villa A., Rosager A.M., Poulsen F.R., Martinez-Serrano A., Zimmer J., Meyer M. // Brain Res. 2009, V.1295, P.1-12
  28. Korzhevskii D.E., Petrova E.S., Kirik O.V., Beznin G.V., Sukhorukova E. G. // Cell Transplantology and Tissue Engineering. 2010, V.5, №3, P.57-63
  29. Korzhevskii D.E., Kirik O.V., Petrova E.S., Karpenko M.N., Grigor’ev I.P., Sukhorukova E.G., Kolos E. A. // Theoretical bases and practical application of the immunohistochemical methods. SPb: SpecLit, 2014 2014, P.119
  30. Wolf H.K., Buslei R., Schmidt-Kastner R., Schmidt-Kastner P.K., Pietsch T., Wiestler O.D., Blümcke I. // J Histochem Cytochem. 1996, V.44, P.1167-1171
  31. Soylemezoglu F., Onder S., Tezel G. G., Berker M. // Pathol Res Pract. 2003, V.199, P.463-468
  32. Darlington P.J., Goldman J. S., Cui Q.L., Antel J.P., Kennedy T.E. // J Neurochem. 2008, V.104, P.1201-1209
  33. Korzhevskii D.E., Gilerovich E.G., Zin’kova N.N., Grigor’ev I.P., Otellin V.A. // Neurosci Behav Physiol. 2006, V.36, №8, P.857-859
  34. Korzhevskii D. E., Sukhorukova E. G., Gilerovich E. G., Petrova E. S., Kirik O. V., Grigor’ev I. P. // Neuroscience and Behavioral Physiology. 2014, V.44, №5, P.542-545
  35. Kolos E. A., Korzhevskii D. E. // Neuroscience and Behavioral Physiology. 2014, V.44, №7, P.790-792
  36. Gill S.K., Ishak M., Rylett R.J. // J Neurosci Methods. 2005, V.148, P.26-35
  37. Korzhevskii D.E., Gilyarov A. V. // Neuroscience and Behavioral Physiology. 2010, V.40, №1, P.107-109
  38. Giliarov A.V., Kirik O.V., Korzhevskii D.E. // Morfologiia. 2010, V.137, №5, P.59-64
  39. Kirik O.V., Sukhorukova E.G., Vlasov T.D., Korzhevskii D.E. // Morfologiia. 2009, V.135, №2, P.80-82
  40. Korzhevskii D.E., Kirik O.V., Baisa A.E., Vlasov T.D. // Bull Exp Biol Med. 2009, V.147, №2, P.255-256
  41. Tippett L.J., Waldvogel H.J., Thomas S.J., Hogg V.M., van Roon-Mom W., Synek B.J., Graybiel A.M., Faull R.L. // Brain. 2007, V.130, P.206-221
  42. Korzhevskii D. E., Khozhai L.I., Gilerovich E. G., Grigoriev I. P., Gilyarov A. V., Otellin V.A. // Proceedings of the All-Russian conference with international participation // Structural, functional and neurochemical regularities of the brain asymmetry and plasticity-2006., Moscow. 2006, P.139-142
  43. Igarashi T., Huang T.T., Noble L.J. // Exp Neurol. 2001, V.172, P.332-341
  44. Davoli M.A., Fourtounis J., Tam J., Xanthoudakis S., Nicholson D., Robertson G.S., Ng G.Y., Xu D. // Neuroscience. 2002, V.115, P.125-136
  45. Unal-Cevik I., Kilinç M., Gürsoy-Ozdemir Y., Gurer G., Dalkara T. // Brain Res. 2004, V.1015, P.169-174

Supplementary files

Supplementary Files
Action
1. JATS XML

Copyright (c) 2015 Gusel’nikova V.V., Korzhevskiy D.E.

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.

This website uses cookies

You consent to our cookies if you continue to use our website.

About Cookies