Email this article Overexpression of MRPS18-2 in Cancer Cell Lines Results in Appearance of Multinucleated Cells
- Authors: Shevchuk Z.1, Yurchenko M.Y.2, Darekar S.D.1, Holodnuka-Kholodnyuk I.3, Kashuba V.I.4, Kashuba E.V.1,2
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Affiliations:
- Department of Microbiology, Tumor and Cell Biology (MTC), Karolinska Institutet
- Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology of NASU
- Kirchenstein Institute of Microbiology and Virology, Riga Stradins University
- Institute of Molecular Biology and Genetics of NASU
- Issue: Vol 5, No 1 (2013)
- Pages: 85-89
- Section: Research Articles
- URL: https://actanaturae.ru/2075-8251/article/view/10607
- DOI: https://doi.org/10.32607/20758251-2013-5-1-85-89
- ID: 10607
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Abstract
Human mitochondrial ribosomal protein MRPS18-2 (S18-2) is encoded by a cellular gene that is located on the human chromosome 6p21.3. We discovered that overexpression of the S18-2 protein led to immortalization and de-differentiation of primary rat embryonic fibroblasts. Cells showed anchorage-independent growth pattern. Moreover, pathways characteristic for rapidly proliferating cells were upregulated then. It is possible that the S18-2 overexpression induced disturbance in cell cycle regulation. We found that overexpression of S18-2 protein in human cancer cell lines led to an appearance of multinucleated cells in the selected clones.
About the authors
Z. Shevchuk
Department of Microbiology, Tumor and Cell Biology (MTC), Karolinska Institutet
Email: Elena.Kashuba@ki.se
Sweden
M. Y. Yurchenko
Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology of NASU
Email: Elena.Kashuba@ki.se
Ukraine
S. D. Darekar
Department of Microbiology, Tumor and Cell Biology (MTC), Karolinska Institutet
Email: Elena.Kashuba@ki.se
Sweden
I. Holodnuka-Kholodnyuk
Kirchenstein Institute of Microbiology and Virology, Riga Stradins University
Email: Elena.Kashuba@ki.se
Latvia
V. I. Kashuba
Institute of Molecular Biology and Genetics of NASU
Email: Elena.Kashuba@ki.se
Ukraine
E. V. Kashuba
Department of Microbiology, Tumor and Cell Biology (MTC), Karolinska Institutet; Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology of NASU
Author for correspondence.
Email: Elena.Kashuba@ki.se
Sweden
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