Transfer of Silver Nanoparticles through the Placenta and Breast Milk during in vivo Experiments on Rats

Cover Page

Abstract


Silver nanoparticles (NPs), widely used in the manufacture of various types of consumer products and for medical applications, belong to novel types of materials that pose potential risks to human health. The potential negative effects of the influence of these NPs on reproduction are insufficiently researched. A quantitative assessment of the transfer of metallic silver nanoparticles through the placenta and breast milk was carried out during an in vivo experiment. We used 34.9 ± 14.8 nm in size silver NPs that were stabilized by low-molecularweight polyvinylpyrrolidone and labeled with the 110mAg radioactive isotope using thermal neutron irradiation in a nuclear reactor. [110mAg]-labeled NPs preparations were administered intragastrically via a gavage needle to pregnant (20th day of gestation) or lactating (14-16th day of lactation) female rats at a dose of 1.69-2.21 mg/kg of body weight upon conversion into silver. The accumulation of NPs in rat fetuses and infant rats consuming their mother’s breast milk was evaluated using a low-background semiconductor gamma-ray spectrometer 24 and 48 hours following labeling, respectively. In all cases, we observed a penetration of the [110mAg]-labeled NPs through the placenta and ther entry into the mother’s milk in amounts exceeding by 100-1,000 times the sensitivity of the utilized analytical method. The average level of accumulation of NPs in fetuses was 0.085-0.147% of the administered dose, which was comparable to the accumulation of the label in the liver, blood, and muscle carcass of adult animals and exceeded the penetration of NPs across the hematoencephalic barrier into the brain of females by a factor of 10-100. In lactating females, the total accumulation of [110mAg]-labeled NPs into the milk exceeded 1.94 ± 0.29% of the administered dose over a 48 h period of lactation; not less than 25% of this amount was absorbed into the gastrointestinal tract of infant rats. Thus, this was the first time experimental evidence of the transfer of NPs from mother to offspring through the placenta and breast milk was obtained.


E. A. Melnik

Federal State Budgetary Institution «Institute of Nutrition» of the Russian Academy of Medical Sciences

Email: gmosh@ion.ru

Russian Federation

Yu. P. Buzulukov

National Research Center Kurchatov Institute

Email: gmosh@ion.ru

Russian Federation

V. F. Demin

National Research Center Kurchatov Institute

Email: gmosh@ion.ru

Russian Federation

V. A. Demin

National Research Center Kurchatov Institute

Email: gmosh@ion.ru

Russian Federation

I. V. Gmoshinski

Federal State Budgetary Institution «Institute of Nutrition» of the Russian Academy of Medical Sciences

Author for correspondence.
Email: gmosh@ion.ru

Russian Federation

N. V. Tyshko

Federal State Budgetary Institution «Institute of Nutrition» of the Russian Academy of Medical Sciences

Email: gmosh@ion.ru

Russian Federation

V. A. Tutelyan

Federal State Budgetary Institution «Institute of Nutrition» of the Russian Academy of Medical Sciences

Email: gmosh@ion.ru

Russian Federation

  1. Vernikov V.M., Gmoshinski I.V., Khotimchenko S.A. Voprosy pitanija. // Problems of nutrition 2009, V.78, №6, P.13-20
  2. Blaser S.A., Scheringer M., Macleod M., Hungerbühler K. // Sci. Total Environ. 2008, V.390, №2-3, P.396-409
  3. Fayaz M.A., Ao Z., Girilal M., Chen L., Xiao X., Kalaichelvan P.T., Yao X. // Int. J. Nanomedicine. 2012, V.7, P.5007-5018
  4. Acosta-Torres L.S., Mendieta I., Nuñez-Anita R.E., Cajero-Juárez M., Castaño V.M. // Int. J. Nanomedicine. 2012, V.7, P.4777-4786
  5. Bhol K.C., Schechter P.J. // Dig. Dis. Sci. 2007, V.52, №10, P.2732-2742
  6. Chrastina A., Schnitzer J.E. // Int. J. Nanomedicine. 2010, V.5, P.653-659
  7. Shumakova A.A., Smirnova V.V., Tananova O.N., Trushina E.N., Kravchenko L.V., Aksenov I.V., Selifanov A.V., Soto G.S., Kuznetsova G.G., Bulachov A.V., Safenkova I.V., Gmoshinski I.V., Khotimchenko S.A. // Voprosy pitanija (Problems of nutrition). 2011, V.80, №6, P.9-18
  8. Kim Y.S., Song M.Y., Park J.D., Song K.S., Ryu H.R., Chung Y.H., Chang H.K., Lee J.H., Oh K.H., Kelman B.J., Hwang I.K., Yu I.J. // Subchronic oral toxicity of silver nanoparticles // Part Fibre Toxicol. 2010, V.7, №1, P.20
  9. Wijnhoven S.W.P., Peijnenburg W.J.G.M., Herberts C.A., Hagens W.I., Oomen A.G., Heugens E.H.W., Roszek B., Bisschops J., Gosens I., Van De Meent D., Dekkers S., De Jong W.H., Van Zijverden M., Sips A.J.A.M., Geertsma R.E. // Nanotoxicology. 2009, V.3, №2, P.109-138
  10. Onischenko G.G., Archakov A.I., Bessonov V.V., Bokit’ko B.G., Gintsburg A.L., Gmoshinski I.V., Grigor’ev A.I., Izmerov N.F., Kirpichnikov M.P., Naroditsky B.S., Pokrovsky V.I., Potapov A.I., Rakhmanin Yu.A., Tutelyan V.A., Khotimchenko S.A. // Gigiena i Sanitaria (Hygiene and Sanitation). 2007, №6, P.3-10
  11. Onischenko G.G., Tutelyan V.A. // Voprosy pitanija (Problems of nutrition). 2007, V.76, №6, P.4-8
  12. Rahman M.F., Wang J., Patterson T.A. // Expression of genes related to oxidative stress in the mouse brain after exposure to silver-25 nanoparticles // Toxicol. Lett. 2009, V.187, №1, P.15-21
  13. Ordzhonikidze C.G., Ramaiyya L.K., Egorova E.M., Rubanovich A.V. // Genotoxic Effects of Silver Nanoparticles on Mice in Vivo // Acta naturae. 2009, V.1, №3, P.99-101
  14. Sung J.H., Ji J.H., Yoon J.U. // Lung function changes in Sprague-Dawley rats after prolonged inhalation exposure to silver nanoparticles // Inhal. Toxicol. 2008, V.20, №6, P.567-574
  15. Ji J.H., Jung J.H., Kim S.S. // Twenty-eight-day inhalation toxicity study of silver nanoparticles in Sprague-Dawley rats // Inhal. Toxicol. 2007, V.19, №10, P.857-871
  16. Oberdörster G., Maynard A., Donaldson K., Castranova V., Fitzpatrick J., Ausman K., Carter J., Karn B., Kreyling W., Lai D., Olin S., Monteiro-Riviere N., Warheit D., Yang H. // Part. and Fibre Toxicol. 2005, V.2, №1, P.8-43
  17. Yokel R.A., MacPhail R.C. // J. Occupational Med. Toxicol. 2011, V.6, №7, P.1-27
  18. Raspopov R.V., Gmoshinski I.V., Popov K.I., Krasnoyarova O.V., Khotimchenko S.A. // Voprosy pitanija (Problems of nutrition). 2012, V.81, №2, P.10-17
  19. Tiede K., Boxall A.B., Tear S.P., Lewis J., David H., Hassellov M. // Food Add.Contam. 2008, V.25, №7, P.795-821
  20. Buzulukov Yu.P., Gmoshinski I.V., Raspopov R.V., Demin V.F., Soloviev V.YU., Kuzmin P.G., Shafeev G.A., Khotimchenko S.A. // Medical Radiol Radiat Safety 2012, V.57, №3, P.5-12
  21. Tyshko N.V., Zhminchenko V.M., Pashorina V.A., Selyaskin K.E., Melnik E.A., Mustafina O.K., Soto S.G., Trushina E.N., Gapparov M.M.G. // Voprosy pitanija (Problems of nutrition). 2011, V.80, №5, P.30-38
  22. // IAEA Database on www.iaea.org/OurWork/NuclearDataService.
  23. Isaev A.G., Babenko V.V., Kazimirov A.S., Grishin S.I., Ievlev S.M. // Problems of nuclear power stations and Chernobyl safety.Kiev. 2010, V.13, P.103-110
  24. Lochamy J.C. // The Minimum Detectable Activity Concept. // EG&G ORTEC Systems Application Studies, PSD,September 1981 1981, №17
  25. Loeschner K., Hadrup N., Qvortrup K., Larse A., Gao X., Vogel U., Mortensen A., Lam H.R., Larsen E.H. // Part. Fibre Toxicol. 2011, V.8, P.1-18
  26. Yang H., Sun C., Fan Z., Tian X., Yan L., Du L., Liu Y., Chen C., Liang X., Anderson G.J., Keela J.A., Zha Y., Nie G. // Sci.Reports. 2012, V.2, №11, P.847-855
  27. Chu M., Wu Q., Yang H., Yuan R., Hou S., Yang Y., Zou Y., Xu S., Xu K., Ji A., Sheng L. // Small. 2010, V.6, №5, P.670-678
  28. Cartwright L., Poulsen M.S., Nielsen H.M., Pojana G., Knudsen L.E., Saunders M., Rytting E. // Int. J. Nanomedicine. 2012, V.7, P.497-510
  29. Kim Y.S., Kim J.S., Cho H.S., Rha D.S., Kim J.M., Park J.D., Choi B.S., Lim R., Chang H.K., Chung Y.H., Kwon I.H., Jeong J., Han B.S., Yu I.J. // Inhal. Toxicol. 2008, V.20, №6, P.575-583
  30. Hussain S.M., Hess K.L., Gearhart J.M., Geiss K.T., Schlager J.J. // Toxicol. in Vitro. 2005, V.19, №7, P.975-983
  31. Braydich-Stolle L., Hussain S., Schlager J.J., Hofmann M.C. // Toxicol. Sci. 2005, V.88, №2, P.412-419
  32. Arora S., Jain J., Rajwade J.M., Paknikar K.M. // Toxicol. Appl. Pharmacol. 2009, V.236, №3, P.31-318
  33. Liu Z., Ren G., Zhang T., Yang Z. // Toxicology. 2009, V.264, №3, P.179-184
  34. Shin S.H., Ye M.K., Kim H.S., Kang H.S. // Int.Immunopharmacol. 2007, V.7, №13, P.1813-1818
  35. Powers C.M., Badireddy A.R., Ryde I.T., Seidler F.J., Slotkin T.A. // Environ. Health Perspect. 2011, V.119, №1, P.37-44
  36. Haase A., Rott S., Mantion A., Graf P., Plendl J., Thünemann A.F., Meier W.P., Taubert A., Luch A., Reiser G. // Toxicol. Sci. 2012, №2, P.457-468

Views

Abstract - 341

PDF (English) - 105

PDF (Russian) - 58

Cited-By


Article Metrics

Metrics Loading ...

Metrics powered by PLOS ALM

PlumX

Dimensions

Refbacks

  • There are currently no refbacks.

Copyright (c) 2013 Melnik E.A., Buzulukov Y.P., Demin V.F., Demin V.A., Gmoshinski I.V., Tyshko N.V., Tutelyan V.A.

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

This website uses cookies

You consent to our cookies if you continue to use our website.

About Cookies