Characteristics of Artificial Virus-like Particles Assembled in vitro from Potato Virus X Coat Protein and Foreign Viral RNAs

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Abstract

Potato virus X (PVX) and some other potexviruses can be reconstituted in vitro from viral coat protein (CP) and RNA. PVX CP is capable of forming viral ribonucleoprotein complexes (vRNP) not only with homologous, but also with foreign RNAs. This paper presents the structure and properties of vRNP assembled in vitro upon incubation of PVX CP and RNAs of various plant and animal viruses belonging to different taxonomic groups. We have shown that the morphology and translational properties of vRNPs containing foreign (heterologous) RNA are identical to those of homological vRNP (PVX RNA - PVX CP). Our data suggest that the assembly of the “mixed” vRNP in vitro could be started at the 5’-proximal region of the RNA, producing a helical structure of vRNPs with foreign nucleic acids. The formation of heterologous vRNP in vitro with PVX CP appears not to require a specific 5’ end RNA nucleotide sequence, and the PVX CP seems to be able to pack foreign genetic material of various sizes and compositions into artificial virus-like particles.

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Characteristics of Artificial Virus-like Particles Assembled in vitro from Potato Virus X Coat Protein and Foreign Viral RNAs
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About the authors

M V Arkhipenko

Lomonosov Moscow State University

Biology Department

E K Petrova

Lomonosov Moscow State University

Biology Department

N A Nikitin

Lomonosov Moscow State University

Biology Department

A D Protopopova

Advanced Technologies Center; Lomonosov Moscow State University

Physical Department

E V Dubrovin

Lomonosov Moscow State University

Physical Department

I V Yaminskii

Advanced Technologies Center; Lomonosov Moscow State University

Physical Department

N P Rodionova

Lomonosov Moscow State University

Biology Department

O V Karpova

Lomonosov Moscow State University

Email: okar@genebee.msu.su
Biology Department

J G Atabekov

Lomonosov Moscow State University; Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University

Biology Department

References

  1. Butler P.J.G. // Philos. Trans. R. Soc. London. B. 1999. V. 354. P. 537.
  2. Klug A. // Philos. Trans. R. Soc. London B. 1999. V. 354. P. 531.
  3. Atabekov J.G. // Nanotechnology in Russia. 2008. V. 3 (1-2). P. 128-137.
  4. Atabekov J.G., Novikov K., Vishnichenko K., Kaftanova A.S. // Virology. 1970. V. 41. P. 519.
  5. Fraenkel-Conrat H., Singer B. // Philos. Trans. R. Soc. London. B. 1999. V. 354. P. 583.
  6. Tollin P., Wilson H.R. // the Filamentous Plant Viruses in the Plant Viruses 4 / Еd. Milne R.C. New York: Plenum Press, 1988. P. 51-83.
  7. Skryabin K.G., Morozov S.Yu., Kraev A.S., Rozanov M.N., Chernov B.K., Lukasheva L.I., Atabekov J.G. // FEBS Lett. 1988. V. 240. P. 33-40.
  8. Morozov S.Yu., Miroshnichenko N.A., Zelenina D.A., Fedorkin O.N., Solovyev A.G., Lukasheva L.I., Karasev A.V., Dolja V.V., Atabekov J.G. // J. Gen. Virol. 1991. V. 72. P. 2039-2043.
  9. Novikov V.K., Kimazev V.Z., Atabekov J.G. // Dokl. Acad. Nauk. SSSR. 1972. V. 204. P. 1259-1262.
  10. Erickson J.W., Bancroft J.B. // Virology. 1978. V. 90. P. 60-66.
  11. Atabekov J.G., Rodionova N.P., Karpova O.V., Kozlovsky S.V., Poljakov V.Y. // Virology. 2000. V. 271. № 2. P. 259-263.
  12. Goodman R.M., Horne R.W., Hobart J.M. // Virology 1975. V. 68. P. 299-308.
  13. Fraenkel-Conrat H., Singer B., Tsugita A. // Virology. 1961. V. 14. P. 54-58.
  14. Karpova O.V., Ivanov K.I., Rodionova N.P., Dorokhov Yu.L., Atabekov J.G. // Virology. 1997. V. 230. P. 11-21.
  15. Karpova O.V., Zayakina O.V., Arkhipenko M.V., Sheval E.V., Kiselyova O.I., Poljakov V.Yu., Yaminsky I.V., Rodionova N.P., Atabekov J.G. // J. Gen. Virol. 2006. V. 87. № 9. P. 2731-2740.
  16. Kaftanova A.S., Kiselev N.A., Novikov V.K., Atabekov J.G. // Virology. 1975. V. 65. P. 283-287.
  17. Kiseleva O.I., Yaminsky I.V., Karpova O.V., Rodionova N.P., Kozlovsky S.V., Arkhipenko M.V., Atabekov J.G. // J. Mol. Biol. 2003. V. 332. № 2. P. 321-325.
  18. Nikitin N.A., Sushko A.D., Arkhipenko M.V., Rodionova N.P., Karpova O.V., Yaminsky I.V. // Colloid Journal. 2011. V. 73 (4). P. 523-530.
  19. Karpova O.V., Arkhipenko M.V., Zayakina O.V., Nikitin N.A., Kiselyova O.I., Kozlovsky S.V., Rodionova N.P., Atabekov J.G. // Mol. Biol. (Mosk.). 2006. V. 40 (4). P. 628-634.
  20. Rodionova N.P., Karpova O.V., Kozlovsky S.V., Zayakina O.V., Arkhipenko M.V., Atabekov J.G. // J. Mol. Biol. 2003. V. 333. № 3. P. 565-572.
  21. Zayakina O., Arkhipenko M., Kozlovsky S., Nikitin N., Smirnov A., Susi P., Rodionova N., Karpova O., Atabekov J. // Mol. Plant. Pathol. 2008. V. 9. № 1. P. 37-44.
  22. Butler P. J. // J. Gen. Virol. 1984. V. 65. № 2. P. 253-279.
  23. Choi Y.G., Rao A.L. // J. Virol. 2003. V. 77. № 18. P. 9750-9757.
  24. Miller E.D., Plante C.A., Kim K.H., Brown J.W., Hemenway C. // J. Mol. Biol. 1998. V. 284. № 3. P. 591-608.
  25. Qu F., Morris T. J. // J. Virol. 1997. V. 71. № 2. P. 1428-1435.
  26. Sit T.L., Leclerc D., AbouHaidar M.G. // Virology. 1994. V. 199. № 1. P. 238-242.
  27. Kwon S.J., Park M.R., Kim K.W., Plante C.A., Hemenway C.L., Kim K.H. // Virology. 2005. V. 334. № 1. P. 83-97.
  28. Lough T. J., Lee R.H., Emerson S.J., Forster R.L., Lucas W.J. // Virology. 2006. V. 351. № 2. P. 455-465.
  29. Lee Y.F., Nomoto A., Detjen B.M., Wimmer E. // Proc. Natl. Acad. Sci. USA. 1977. V. 74. № 1. P. 59-63.
  30. Yogo Y., Wimmer E. // Proc. Natl. Acad. Sci. USA. 1972. V. 69. № 7. P. 1877-1882.
  31. Abouhaidar M.G., Bancroft J.B. // Virology. 1980. V. 107. № 1. P. 202-207.

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Copyright (c) 2011 Arkhipenko M.V., Petrova E.K., Nikitin N.A., Protopopova A.D., Dubrovin E.V., Yaminskii I.V., Rodionova N.P., Karpova O.V., Atabekov J.G.

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