Email this article Analogues of Artificial Human Box C/D Small Nucleolar RNA As Regulators of Alternative Splicing of a pre-mRNA Target
- Authors: Stepanov G.A.1, Semenov D.V.1, Kuligina E.V.1, Koval O.A.1, Rabinov I.V.1, Kit Y.Y.2, Richter V.A.1
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Affiliations:
- Institute of Chemical Biology and Fundamental Medicine, Siberian Branch, Russian Academy of Sciences
- Institute of Cell Biology, National Academy of Sciences of Ukraine
- Issue: Vol 4, No 1 (2012)
- Pages: 32-41
- Section: Research Articles
- URL: https://actanaturae.ru/2075-8251/article/view/10631
- DOI: https://doi.org/10.32607/20758251-2012-4-1-32-41
- ID: 10631
Cite item
Abstract
Small nucleolar RNAs (snoRNAs) play a key role in ribosomal RNA (rRNA) biogenesis. Box C/D snoRNAs guide the site-specific 2’-O-ribose methylation of nucleotides in rRNAs and small nuclear RNAs (snRNAs). A number of box C/D snoRNAs and their fragments have recently been reported to regulate post-transcriptional modifications and the alternative splicing of pre-mRNA. Artificial analogues of U24 snoRNAs directed to nucleotides in 28S and 18S rRNAs, as well as pre-mRNAs and mature mRNAs of human heat shock cognate protein (hsc70), were designed and synthesized in this study. It was found that after the transfection of MCF-7 human cells with artificial box C/D RNAs in complex with lipofectamine, snoRNA analogues penetrated into cells and accumulated in the cytoplasm and nucleus. It was demonstrated that the transfection of cultured human cells with artificial box C/D snoRNA targeted to pre-mRNAs induce partial splicing impairments. It was found that transfection with artificial snoRNAs directed to 18S and 28S rRNA nucleotides, significant for ribosome functioning, induce a decrease in MCF-7 cell viability.
About the authors
G. A. Stepanov
Institute of Chemical Biology and Fundamental Medicine, Siberian Branch, Russian Academy of Sciences
Author for correspondence.
Email: stepanovga@niboch.nsc.ru
Russian Federation
D. V. Semenov
Institute of Chemical Biology and Fundamental Medicine, Siberian Branch, Russian Academy of Sciences
Email: stepanovga@niboch.nsc.ru
Russian Federation
E. V. Kuligina
Institute of Chemical Biology and Fundamental Medicine, Siberian Branch, Russian Academy of Sciences
Email: stepanovga@niboch.nsc.ru
Russian Federation
O. A. Koval
Institute of Chemical Biology and Fundamental Medicine, Siberian Branch, Russian Academy of Sciences
Email: stepanovga@niboch.nsc.ru
Russian Federation
I. V. Rabinov
Institute of Chemical Biology and Fundamental Medicine, Siberian Branch, Russian Academy of Sciences
Email: stepanovga@niboch.nsc.ru
Russian Federation
Yu. Ya. Kit
Institute of Cell Biology, National Academy of Sciences of Ukraine
Email: stepanovga@niboch.nsc.ru
Ukraine
V. A. Richter
Institute of Chemical Biology and Fundamental Medicine, Siberian Branch, Russian Academy of Sciences
Email: stepanovga@niboch.nsc.ru
Russian Federation
References
- Bachellerie J.P., Cavaille J., Huttenhofer A. // Biochimie. 2002. V. 84. P. 775-790.
- Cavaille J., Nicoloso M., Bachellerie J.P. // Nature. 1996. V. 383. P. 732-735.
- Liu B., Fournier M. // RNA. 2004. V. 10. P. 1130-1141.
- Liu B., Ni J., Fournier M. // Methods. 2001. V. 23. P. 276-286.
- Vitali P., Basyuk E., Le M.E., Bertrand E., Muscatelli F., Cavaille J., Huttenhofer A. // J. Cell. Biochem. 2005. V. 169. P. 745-753.
- Kishore S., Stamm S. // Science. 2006. V. 311. P. 230-232.
- Kiss-Laszlo Z., Henry Y., Bachellerie J.P., Caizergues-Ferrer M., Kiss T. // Cell. 1996. V. 85. P. 1077-1088.
- Maden B. // Methods. 2001. V. 25. P. 374-382.
- Qu L.H., Henry Y., Nicoloso M., Michot B., Azum M.C., Renalier M.H., Caizergues-Ferrer M., Bachellerie J.P. // Nucl. Acids Res. 1995. V. 23. P. 2669-2676.
- Rohde M., Daugaard M., Jensen M.H., Helin K., Nylandsted J., Jaattela M. // Genes Dev. 2005. V. 19. P. 570-582.
- Decatur W.A., Fournier M.J. // Trends Biochem. Sci. 2002. V. 27. P. 344-351.
- King T.H., Liu B., McCully R.R., Fournier M.J. // Mol. Cell. 2003. V. 11. P. 425-435.
- Baxter-Roshek J.L., Petrov A.N., Dinman J.D. // PLoS ONE. 2007. V. 2. e174.
- Graifer D.M., Karpova G.G., Knorre D.G. // Biochemistry. 2001. V. 66. P. 585-602.
- Kiss-Laszlo Z., Henry Y., Kiss T. // EMBO J. 1998. V. 17. P. 797-807.
- Moore M., Sharp P. // Science. 1992. V. 256. P. 992-997.
- Dominski Z., Kole R. // Mol. Cell. Biol. 1994. V. 14. P. 7445-7454.
- Sazani P., Kole R. // J. Clin. Invest. 2003. V. 112. P. 481-486.
- Zalachoras I., Evers M.M., van Roon-Mom W.M., Aartsma-Rus A.M., Meijer O.C. // Front. Mol. Neurosci. 2011. V. 4. P. 1-12.
- Semenov D.V., Stepanov G.A., Baryakin D.N., Koval O.A., Kuligina E.V., Richter V.A. // Circulating Nucleic Acids in Plasma and Serum / Ed. Gahan P.B. Springer Science+Business Media B.V. 2011. P. 233-237.
- Semenov D.V., Vratskih O.V., Kuligina E.V., Richter V.A. // Ann. N.Y. Acad. Sci. 2008. V. 1137. P. 119-124.
- Watkins N.J., Segault V., Charpentier B., Nottrott S., Fabrizio P., Bachi A., Wilm M., Rosbash M., Branlant C., Luhrmann R. // Cell. 2000. V. 27. V. 103. P. 457-466.
- Tollervey D., Lehtonen H., Jansen R., Kern H., Hurt E.C. // Cell. 1993. V. 72. P. 443-457.
- Newman D.R., Kuhn J.F., Shanab G.M., Maxwell E.S. // RNA. 2000. V. 6. P. 861-879.
- Liu B., Liang X.-H., Piekna-Przybylska D. // RNA Biol. 2008. V. 5. P. 249-254.
- Ganot P., Bortolin M.L., Kiss T. // Cell. 1997. V. 89. P. 799-809.
- Panse V.G., Johnson A.W. // Trends Biochem. Sci. 2010. V. 35. P. 260-266.
- Freed E., Bleichert F., Dutca L., Baserga S. // Mol. Biosyst. 2010. V. 6. P. 481-493.
- Vanacova S., Stefl R. // EMBO Rep. 2007. V. 8. P. 651-657.
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