Email this article Cancer Specificity of Promoters of the Genes Involved in Cell Proliferation Control
- Authors: Kashkin K.N.1, Chernov I.P.1, Stukacheva E.A.1, Kopantzev E.P.1, Monastyrskaya G.S.1, Uspenskaya N.Y.1, Sverdlov E.D.1
-
Affiliations:
- Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry
- Issue: Vol 5, No 3 (2013)
- Pages: 79-83
- Section: Research Articles
- URL: https://actanaturae.ru/2075-8251/article/view/10587
- DOI: https://doi.org/10.32607/20758251-2013-5-3-79-83
- ID: 10587
Cite item
Abstract
Core promoters with adjacent regions of the human genes CDC6, POLD1, CKS1B, MCM2, and PLK1 were cloned into a pGL3 vector in front of the Photinus pyrails gene Luc in order to study the tumor specificity of the promoters. The cloned promoters were compared in their ability to direct luciferase expression in different human cancer cells and in normal fibroblasts. The cancer-specific promoter BIRC5 and non-specific CMV immediately early gene promoter were used for comparison. All cloned promoters were shown to be substantially more active in cancer cells than in fibroblasts, while the PLK1 promoter was the most cancer-specific and promising one. The specificity of the promoters to cancer cells descended in the series PLK1, CKS1B, POLD1, MCM2, and CDC6. The bidirectional activity of the cloned CKS1B promoter was demonstrated. It apparently directs the expression of the SHC1 gene, which is located in a “head-to-head” position to the CKS1B gene in the human genome. This feature should be taken into account in future use of the CKS1B promoter. The cloned promoters may be used in artificial genetic constructions for cancer gene therapy.
Keywords
About the authors
K. N. Kashkin
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry
Author for correspondence.
Email: kachkine@yandex.ru
Russian Federation
I. P. Chernov
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry
Email: kachkine@yandex.ru
Russian Federation
E. A. Stukacheva
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry
Email: kachkine@yandex.ru
Russian Federation
E. P. Kopantzev
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry
Email: kachkine@yandex.ru
Russian Federation
G. S. Monastyrskaya
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry
Email: kachkine@yandex.ru
Russian Federation
N. Ya. Uspenskaya
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry
Email: kachkine@yandex.ru
Russian Federation
E. D. Sverdlov
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry
Email: kachkine@yandex.ru
Russian Federation
References
- Kawakami H., Katayama T. // Biochem Cell Biol. 2010, V.88, P.49-62
- Borlado L.R., Mendez J. // Carcinogenesis. 2008, V.29, P.237-243
- Lange S.S., Takata K., Wood R.D. // Nat Rev Cancer. 2011, №11, P.96-110
- Martinsson-Ahlzen H.S., Liberal V., Grunenfelder B., Chaves S.R., Spruck C.H., Reed S.I. // Mol Cell Biol. 2008, V.28, P.5698-5709
- Krishnan A., Nair S.A., Pillai M.R. // J Cell Mol Med. 2010, V.14, P.154-164
- Davila Lopez M., Martinez Guerra J.J., Samuelsson T. // PLoS One. 2010, V.5, P.e10654
- Rajendran M., Thomes P., Zhang L., Veeramani S., Lin M.F. // Cancer Metastasis Rev. 2010, V.29, P.207-222
- Masai H., You Z., Arai K. // IUBMB Life. 2005, V.57, P.323-335
- Song B., Liu X.S., Liu X. // Cell Div. 2012, V.7, P.3
- Zhang Y., Luoh S.M., Hon L.S., Baertsch R., Wood W.I., Zhang Z. // Nucleic Acids Res. 2007, V.35, P.152-158
- Kopantzev E.P., Vayshlya N.A., Kopantseva M.R., Egorov V.I., Pikunov M., Zinovyeva M.V., Vinogradova T.V., Zborovskaya I.B., Sverdlov E.D. // Br J Cancer. 2010, V.102, P.1533-1540
- Mityaev M.V., Kopantzev E.P., Buzdin A.A., Vinogradova T.V., Sverdlov E.D. // Biochemistry (Mosc). 2010, V.75, P.182-191
Supplementary files
