Characterization of the C6D7-RBD Human Monoclonal Antibody Specific to the SARS-CoV-2 S Protein Receptor-Binding Domain
| Dublin Core | PKP Metadata Items | Metadata for this Document | |
| 1. | Title | Title of document | Characterization of the C6D7-RBD Human Monoclonal Antibody Specific to the SARS-CoV-2 S Protein Receptor-Binding Domain |
| 2. | Creator | Author's name, affiliation, country | Yana O. Romanenko; Federal Budget Institution of Science State Research Center for Applied Microbiology and Biotechnology; Россия |
| 2. | Creator | Author's name, affiliation, country | Marina V. Silkina; Federal Budget Institution of Science State Research Center for Applied Microbiology and Biotechnology; Россия |
| 2. | Creator | Author's name, affiliation, country | Alena S. Kartseva; Federal Budget Institution of Science State Research Center for Applied Microbiology and Biotechnology; Россия |
| 2. | Creator | Author's name, affiliation, country | Maksim A. Marin; Federal Budget Institution of Science State Research Center for Applied Microbiology and Biotechnology; Россия |
| 2. | Creator | Author's name, affiliation, country | Maria A. Shkuratova; Federal Budget Institution of Science State Research Center for Applied Microbiology and Biotechnology; Россия |
| 2. | Creator | Author's name, affiliation, country | Maria A. Makarova; Federal Budget Institution of Science State Research Center for Applied Microbiology and Biotechnology; Россия |
| 2. | Creator | Author's name, affiliation, country | Alena K. Ryabko; Federal Budget Institution of Science State Research Center for Applied Microbiology and Biotechnology; Россия |
| 2. | Creator | Author's name, affiliation, country | Daria A. Konyshkova; Federal Budget Institution of Science State Research Center for Applied Microbiology and Biotechnology; Россия |
| 2. | Creator | Author's name, affiliation, country | Natalia A. Zeninskaya; Federal Budget Institution of Science State Research Center for Applied Microbiology and Biotechnology; Россия |
| 2. | Creator | Author's name, affiliation, country | Anna E. Khlyntseva; Federal Budget Institution of Science State Research Center for Applied Microbiology and Biotechnology; Россия |
| 2. | Creator | Author's name, affiliation, country | Igor G. Shemyakin; Federal Budget Institution of Science State Research Center for Applied Microbiology and Biotechnology; Россия |
| 2. | Creator | Author's name, affiliation, country | Victoria V. Firstova; Federal Budget Institution of Science State Research Center for Applied Microbiology and Biotechnology; Россия |
| 3. | Subject | Discipline(s) | |
| 3. | Subject | Keyword(s) | COVID-19; SARS-CoV-2; receptor-binding domain; human monoclonal antibody; virus-neutralizing activity |
| 4. | Description | Abstract | The new coronavirus infection COVID-19 is an acute viral disease that affects primarily the upper respiratory tract. The etiological agent of COVID-19 is the SARS-CoV-2 RNA virus (Coronaviridae family, Betacoronavirus genus, Sarbecovirus subgenus). We have developed a high-affinity human monoclonal antibody, called C6D7-RBD, which is specific to the S protein receptor-binding domain (RBD) from the SARS-CoV-2 Wuhan-Hu-1 strain and exhibits virus-neutralizing activity in a test with recombinant antigens: angiotensin-converting enzyme 2 (ACE2) and RBD. |
| 5. | Publisher | Organizing agency, location | Acta Naturae Ltd |
| 6. | Contributor | Sponsor(s) |
Government of the Russian Federation |
| 7. | Date | (DD-MM-YYYY) | 03.05.2023 |
| 8. | Type | Status & genre | Peer-reviewed Article |
| 8. | Type | Type | Research Article |
| 9. | Format | File format | |
| 10. | Identifier | Uniform Resource Identifier | https://actanaturae.ru/2075-8251/article/view/11849 |
| 10. | Identifier | Digital Object Identifier (DOI) | 10.32607/actanaturae.11849 |
| 11. | Source | Title; vol., no. (year) | Acta Naturae; Vol 15, No 1 (2023) |
| 12. | Language | English=en | ru |
| 13. | Relation | Supp. Files |
Fig. 1. Electrophoregram of C6D7-RBD hMAb.1 – PageRuler™ SM1811 molecular weight markers (Fermentas, USA).2 – C6D7-RBD hMAb sample, reducing conditions.3 – C6D7-RBD hMAb sample, non-reducing conditions (102KB) doi: 10.32607/20758251-2023-15-1-81-86-68237 Fig. 2. C6D7-RBD hMAb specificity to the recombinant RBD protein.1 – PageRuler™ SM1811 molecular weight markers (Fermentas, USA).2 – Recombinant RBD detected with C6D7-RBD hMAb (72KB) doi: 10.32607/20758251-2023-15-1-81-86-68238 Fig. 3. Class assignment of C6D7-RBD hMAb.1 – Strip for determining the light chain type and immunoglobulin class.2 – Strip for determining the IgG subclass (159KB) doi: 10.32607/20758251-2023-15-1-81-86-68239 Fig. 4. Determining KD for C6D7-RBD hMAb and the target RBD protein (58KB) doi: 10.32607/20758251-2023-15-1-81-86-68240 Fig. 5. RBD–ACE2-neutralizing activity of C6D7-RBD hMAb (68KB) doi: 10.32607/20758251-2023-15-1-81-86-68241 |
| 14. | Coverage | Geo-spatial location, chronological period, research sample (gender, age, etc.) | |
| 15. | Rights | Copyright and permissions |
Copyright (c) 2023 Romanenko Y.O., Silkina M.V., Kartseva A.S., Marin M.A., Shkuratova M.A., Makarova M.A., Ryabko A.K., Konyshkova D.A., Zeninskaya N.A., Khlyntseva A.E., Shemyakin I.G., Firstova V.V. This work is licensed under a Creative Commons Attribution 4.0 International License. |