Acta NaturaeActa NaturaeActa Naturae2075-8251Acta Naturae Ltd2788910.32607/actanaturae.27889Research ArticlesЭкспериментальные статьиResearch ArticleA comparative study of genital lichen sclerosus transcriptomesСравнительное исследование транскриптомов генитального склероатрофического лихенаMargasyukS. D.МаргасюкС. Д.
Russian Federation
pervouchine@gmail.comKunetsovaA. L.КузнецоваА. Л.
Russian Federation
pervouchine@gmail.comSkvortsovD. A.СкворцовД. А.
Russian Federation

Faculty of Chemistry

химический факультет

pervouchine@gmail.comAlekberovE. M.АлекберовЭ. М.
Russian Federation
pervouchine@gmail.comIritsyanM. M.ИрицянМ. М.
Russian Federation
pervouchine@gmail.comPulbereS. A.ПульбереС. А.
Russian Federation
pervouchine@gmail.comKotovS. V.КотовС. В.
Russian Federation
pervouchine@gmail.comSokolovaA. A.СоколоваА. А.
Russian Federation
pervouchine@gmail.comPervouchineD. D.ПервушинД. Д.
Russian Federation

Faculty of Chemistry

химический факультет

pervouchine@gmail.comCenter for Molecular and Cellular BiologyЦентр молекулярной и клеточной биологииMoscow State UniversityМосковский государственный университет им. М.В. ЛомоносоваN.I. Pirogov Russian National Research Medical UniversityРоссийский национальный исследовательский медицинский университет им. Н.И. ПироговаN.I. Pirogov City Clinical Hospital No. 1 of the Moscow Department of HealthГородская клиническая больница № 1 им. Н.И. ПироговаYevdokimov Moscow State University of Medicine and DentistryМосковский государственный медико-стоматологический университет им. А.И. Евдокимова2307202618285961911202511022026Copyright ©; 2026, Margasyuk S.D., Kunetsova A.L., Skvortsov D.A., Alekberov E.M., Iritsyan M.M., Pulbere S.A., Kotov S.V., Sokolova A.A., Pervouchine D.D.Copyright ©; 2026, Маргасюк С.Д., Кузнецова А.Л., Скворцов Д.А., Алекберов Э.М., Ирицян М.М., Пульбере С.А., Котов С.В., Соколова А.А., Первушин Д.Д.2026Margasyuk S.D., Kunetsova A.L., Skvortsov D.A., Alekberov E.M., Iritsyan M.M., Pulbere S.A., Kotov S.V., Sokolova A.A., Pervouchine D.D.Маргасюк С.Д., Кузнецова А.Л., Скворцов Д.А., Алекберов Э.М., Ирицян М.М., Пульбере С.А., Котов С.В., Соколова А.А., Первушин Д.Д.https://creativecommons.org/licenses/by/4.0https://actanaturae.ru/2075-8251/article/view/27889

Genital lichen sclerosus (GLS) is a chronic inflammatory dermatosis that affects the genital skin. Despite different clinical manifestations, the pathogenesis of GLS in men and women is believed to be common and is attributed to a combination of autoimmune and genetic factors. In this study, we compared the transcriptomic profiles of penile (mGLS) and vulvar lichen sclerosus (VLS), aiming to identify commonly deregulated genes. We observed a substantial heterogeneity in the transcriptomic signatures in mGLS samples, which is driven by different compositions of immune infiltrates. In mGLS, gene expression signatures strongly indicate epidermis dysfunction and overexpression of the epithelial inflammation marker Keratin 6 (KRT6) and chitinase CHIT1. No significant changes in the expression levels of known GLS markers, such as VIM, CTNNB1, LGALS7 and ECM1, were detected. However, significant changes in the expression levels of the genes associated with autoimmune diseases and the genes upregulated in squamous cell carcinoma, including TNF, CCNB1 and RUNX3, were observed. There was no enrichment in the polyU/UC insertions that were reported previously. Instead, we have identified a long non-coding RNA DRAIC with a high coding potential that is commonly upregulated in mGLS and VLS. Taken together, our results provide a comprehensive picture of the shared transcriptomic signatures, including novel biomarkers and potential therapeutic targets.

Генитальный склероатрофический лихен (GLS) – это хронический воспалительный дерматоз, поражающий кожу гениталий. Несмотря на различные клинические проявления, патогенез GLS у мужчин и у женщин считается общим и обусловлен сочетанием аутоиммунных и генетических факторов. C целью выявления общих нарушений регуляции экспрессии генов сравнили транскриптомные профили пенильного (mGLS) и вульварного склероатрофического лихена (VLS). Выявили значительную гетерогенность транскриптомных сигнатур в образцах mGLS, что обусловлено различиями в составе иммунных инфильтратов. Сигнатуры экспрессии генов в mGLS указывают на дисфункцию эпидермиса и повышенную экспрессию генов кератина 6 (KRT6), маркера эпителиального воспаления, а также хитиназы (CHIT1). Не обнаружено значимых изменений в уровнях экспрессии таких известных маркеров GLS, как VIM, CTNNB1, LGALS7 и ECM1, однако наблюдались изменения в уровнях экспрессии генов, связанных с аутоиммунными заболеваниями, и в генах, активируемых при плоскоклеточном раке легкого, включая гены TNF, CCNB1 и RUNX3. Не обнаружено обогащения инсерциями поли-U/UC, о которых сообщалось ранее. Идентифицирована длинная некодирующая РНК DRAIC с высоким кодирующим потенциалом, экспрессия которой значительно повышена и в mGLS, и в VLS. В совокупности нами получен полный каталог общих транскриптомных сигнатур mGLS и VLS, включая новые биомаркеры и потенциальные терапевтические мишени.

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