Acta NaturaeActa NaturaeActa Naturae2075-8251Acta Naturae Ltd1083110.32607/20758251-2009-1-1-109-114ArticlesСтатьиResearch ArticleThe Production and Characteristics of a Mouse’s Embryonic Stem Cell Lineage, Transfected by the Glia Neurotrophic Factor and Gene Fused with the Green Fluorescent Protein GeneThe Production and Characteristics of a Mouse’s Embryonic Stem Cell Lineage, Transfected by the Glia Neurotrophic Factor and Gene Fused with the Green Fluorescent Protein GeneArsenievaE Ligorag@img.ras.ruKuzminI Vigorag@img.ras.ruManuilovaE Sigorag@img.ras.ruNovosadovaE Vigorag@img.ras.ruMurkinE Vigorag@img.ras.ruPavlovaG Vigorag@img.ras.ruTarantulV Zigorag@img.ras.ruGrivennikovI Aigorag@img.ras.ruMolecular Genetics Institute, Russian Academy of SciencesGene Biology Institute, Russian Academy of SciencesMolecular Genetics Institute, Russian Academy of SciencesGene Biology Institute, Russian Academy of Sciences1506200911NO1 (2009)№1 (2009)10911417012020Copyright ©; 2009, Arsenieva E.L., Kuzmin I.V., Manuilova E.S., Novosadova E.V., Murkin E.V., Pavlova G.V., Tarantul V.Z., Grivennikov I.A.Copyright ©; 2009, Arsenieva E.L., Kuzmin I.V., Manuilova E.S., Novosadova E.V., Murkin E.V., Pavlova G.V., Tarantul V.Z., Grivennikov I.A.2009Arsenieva E.L., Kuzmin I.V., Manuilova E.S., Novosadova E.V., Murkin E.V., Pavlova G.V., Tarantul V.Z., Grivennikov I.A.Arsenieva E.L., Kuzmin I.V., Manuilova E.S., Novosadova E.V., Murkin E.V., Pavlova G.V., Tarantul V.Z., Grivennikov I.A.https://creativecommons.org/licenses/by/4.0https://actanaturae.ru/2075-8251/article/view/10831

The influence that the expression of the human (glial-derived neurotrophic factor (GDNF)) neurotrophic factor has on the morphology and proliferative activity of embryonic stem cells (SC) of a mouse with R1 lineage, as well as their ability to form embroid bodies (EB), has been studied. Before that, using a PCR (polymerase chain reaction) coupled with reverse transcription, it was shown that, in this very lineage of the embryonic SC, the expression of the receptors’ genes is being fulfilled for the neurotropfic RET and GFRα1 glia factor. The mouse's embryonic SC lineage has been obtained, transfected by the human GDNF gene, and has been fused with the “green” fluorescent protein (GFP) gene. The presence of the expression of the human GDNF gene in the cells was shown by northern hybridization and the synthesis of its albuminous product by immunocitochemical coloration with the use of specific antibodies. The reliable slowing-down of the embriod-body formation by the embryonic SC transfected by the GDNF gene has been shown. No significant influence of the expression of the GDNF gene on the morphology and the proliferative activity of the transfected embryonic SCs has been found when compared with the control ones.

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