Acta NaturaeActa NaturaeActa Naturae2075-8251Acta Naturae Ltd1081910.32607/20758251-2009-1-2-86-90ArticlesСтатьиResearch ArticleA Comparison of Target gene Silencing using Synthetically Modified siRNA and shRNA That Express Recombinant Lentiviral VectorsA Comparison of Target gene Silencing using Synthetically Modified siRNA and shRNA That Express Recombinant Lentiviral VectorsSpirinP VBaskaranDRubtsovP MZenkovaM AVlassovV VChernolovskayaE LPrassolovV Sprasolov@eimb.ruEngelghardt Institute of Molecular Biology, Russian Academy of SciencesInstitute of Chemical Biology and Fundamental Medicine, Siberian Branch, Russian Academy of SciencesInstitute of Chemical Biology and Fundamental Medicine, Siberian Branch, Russian Academy of SciencesEngelghardt Institute of Molecular Biology, Russian Academy of Sciences1509200912NO2 (2009)№2 (2009)869017012020Copyright ©; 2009, Spirin P.V., Baskaran D., Rubtsov P.M., Zenkova M.A., Vlassov V.V., Chernolovskaya E.L., Prassolov V.S.Copyright ©; 2009, Spirin P.V., Baskaran D., Rubtsov P.M., Zenkova M.A., Vlassov V.V., Chernolovskaya E.L., Prassolov V.S.2009Spirin P.V., Baskaran D., Rubtsov P.M., Zenkova M.A., Vlassov V.V., Chernolovskaya E.L., Prassolov V.S.Spirin P.V., Baskaran D., Rubtsov P.M., Zenkova M.A., Vlassov V.V., Chernolovskaya E.L., Prassolov V.S.https://creativecommons.org/licenses/by/4.0https://actanaturae.ru/2075-8251/article/view/10819

RN A interference is an efficient natural mechanism of gene expression modulation on the post translation level that was revealed both in higher animal and plant eukaryotes and in lower level eukaryotes and viruses. At present RN A interference is used as a powerful instrument in research of the functional activity of the genes, and with the help of this instrument findings have been obtained that are of significant importance for many areas of fundamental biology. At the same time, developments are under way in many world laboratories aimed at creating new-generation therapeutic means able to treat inherited, malignant and inflectional diseases of different aetiology based on the usage of the interfering RN As. One of the major problems of these researches is the retrieval of the efficient techniques able to deliver the interfering RN As into the target cells. At present for the introduction of the interfering RN As into the cells transfection and transduction are used with the help of the viral vectors that direct the shRN A synthesis in the cells, which are the predecessors of the corresponding siRN As. In the article findings are given of the comparison the efficiency of the oncogene AML1-ET O suppression with the help of lipofection of the synthetic siRN A and also while using the lentiviral vector which directs the shRN A synthesis – the anti AML1- ET O siРНК predecessor.

retroviral vectorsRN A interferenceembryonic mouse fibroblastsVilgelm A.E., Chumakov S.P., Prasolov V.S. // Molecular Biology 2006. Vol. 40. -3 pages 1-18.Volkov A.A., Kruglova N.S., Meschaninova M.I., et al. // Oligonucleotides. 2009. Jun;19(2):191-202.Shuey D.J., McCallus D.E., Giordano T. // Drug Discov. Today. 20027. 1040–1046.Stanisławska J., Olszewski W.L. // Arch. Immunol. Ther. Exp. 200553. 39–46.Elbashir S.M., Harborth J., Weber K., Tuschl T. // Methods. 200226. 199–213.Brummelkamp T.R., Bernards R., Agami R. // Science. 2002296. 550–553.Satake N., Maseki N., Kozu T., et al. // British Journ. of Haemat. 199591: 892-898.Peterson L.F., Zhang D.E. // Oncogene. 2004; 23(24):4255-62.Kravchenko J.E., Ilyinskaya G.V., Komarov P.G., et al. // Proc. Natl. Acad. Sci. USA. 2008. Apr 29;105(17):6302-7.