Acta NaturaeActa NaturaeActa Naturae2075-8251Acta Naturae Ltd1081810.32607/20758251-2009-1-2-81-85ArticlesСтатьиResearch ArticleTelomerase Complex from Yeast Saccharomyces cerevisiae Contains a Biotinylated ComponentTelomerase Complex from Yeast Saccharomyces cerevisiae Contains a Biotinylated ComponentShcherbakovaD MZverevaM Izvereva@genebee.msu.suDontsovaO ADepartment of Chemistry, Moscow State University1509200912NO2 (2009)№2 (2009)818517012020Copyright ©; 2009, Shcherbakova D.M., Zvereva M.I., Dontsova O.A.Copyright ©; 2009, Shcherbakova D.M., Zvereva M.I., Dontsova O.A.2009Shcherbakova D.M., Zvereva M.I., Dontsova O.A.Shcherbakova D.M., Zvereva M.I., Dontsova O.A.https://creativecommons.org/licenses/by/4.0https://actanaturae.ru/2075-8251/article/view/10818

Telomerase adds telomeric repeats to single-stranded DNA at the ends of the chromosomes. This enzyme is a ribonucleoprotein complex. Telomerase from yeast Saccharomyces cerevisiae consists of TLC1 RN A, which serves as a template for the synthesis of telomeric repeats, telomerase reverse transcriptase Est2p, and a number of accessory proteins (Est1p, Est3p, Ku70/Ku80, and Sm-complex). We found that the yeast telomerase complex contains a biotinylated component. The telomerase fraction containing biotinylated protein is active in vitro and constitutes a small part of the total amount of active telomerase isolated from cells. We speculate about the nature of the biotinylated component.

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