Acta NaturaeActa NaturaeActa Naturae2075-8251Acta Naturae Ltd1079210.32607/20758251-2009-1-3-121-124ArticlesСтатьиResearch ArticleDeamination of 5-Methylcytosine Residues in Mammalian CellsDeamination of 5-Methylcytosine Residues in Mammalian CellsGromenkoE VSpirinP VKubarevaE ARomanovaE APrassolovV SShpanchenkoO VDontsovaO Aolgash@genebee.msu.ruDepartment of Chemistry, Lomonosov Moscow State UniversityEngelhardt Institute of Molecular Biology, Russian Academy of SciencesBelozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State UniversityBelozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University1512200913NO3 (2009)№3 (2009)12112417012020Copyright ©; 2009, Gromenko E.V., Spirin P.V., Kubareva E.A., Romanova E.A., Prassolov V.S., Shpanchenko O.V., Dontsova O.A.Copyright ©; 2009, Gromenko E.V., Spirin P.V., Kubareva E.A., Romanova E.A., Prassolov V.S., Shpanchenko O.V., Dontsova O.A.2009Gromenko E.V., Spirin P.V., Kubareva E.A., Romanova E.A., Prassolov V.S., Shpanchenko O.V., Dontsova O.A.Gromenko E.V., Spirin P.V., Kubareva E.A., Romanova E.A., Prassolov V.S., Shpanchenko O.V., Dontsova O.A.https://creativecommons.org/licenses/by/4.0https://actanaturae.ru/2075-8251/article/view/10792

DNA demethylation in mammalia occurs after fertilization and during embryogenesis and accompanies cell aging and cancer transformation. With the help of the primer extension reaction, MALDI MS and DNA cleavage by thymine DNA glycosylase deamination of 5-methylcytosine residues has been shown to take place when the model methylated DNA duplexes are treated with nuclear extracts from the cell lines СНO, HeLa, and Skov3. The hypothesis that deamination of 5-methylcytosine is the first stage of demethylation in mammalia has been postulated.

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