Acta NaturaeActa Naturae2075-8251Acta Naturae Ltd1078910.32607/20758251-2009-1-3-102-107Research ArticleCombining Two Technologies for Full Genome Sequencing of HumanSkryabinK G-ProkhortchoukE Bprokhortchouk@biengi.ac.ruMazurA M-BoulyginaE S-TsygankovaS V-NedoluzhkoA V-RastorguevS M-MatveevV B-ChekanovN N-GoranskayaD A-TeslyukA B-GruzdevaN M-VelikhovV E-ZaridzeD G-KovalchukM V-Russian Research Centre Kurchatov InstituteInstitute of Carcinogenesis, Blokhin Cancer Research Center, Russian Academy of Medical SciencesBioengineering Center, Russian Academy of Sciences151220091310210717012020Copyright © 2009, Skryabin K.G., Prokhortchouk E.B., Mazur A.M., Boulygina E.S., Tsygankova S.V., Nedoluzhko A.V., Rastorguev S.M., Matveev V.B., Chekanov N.N., Goranskaya D.A., Teslyuk A.B., Gruzdeva N.M., Velikhov V.E., Zaridze D.G., Kovalchuk M.V.2009human genomesequencing technologysingle-nucleotide polymorphismProgress in the development of novel DNA sequencing technologies allowing rapid and accurate determination of the distinctive features of an individual at the level of the structure of his genome has made genomics one of the most rapidly developing scientific disciplines. At present, three advanced technologies of DNA sequencing are in use: pyrosequencing based on the “sequencing-by-synthesis” principle and commercialized in a next-generation Roche GS-FLX capillary genome sequencing system (454 Life Science Inc./Roche), cyclic sequencing by oligonucleotide ligation and detection (SOLiD, Applied Biosystems), and high-throughput DNA molecular cluster sequencing-by-synthesis using proprietary fluorescently labeled oligonucleotides (Illumina GAII Genome Analyzer, previously known as SOLEXA). These platforms have already demonstrated their significant worth: in the last two years, five new genomes have been read – in addition to the reference genome sequence that was determined by several leading research groups at institutes in the U.S., United Kingdom, and Canada in the course of 10 years and at a cost of 3 bln dollars [1] – such as the genomes of outstanding biologists of our times[2, 3], that of a Nigerian man [4, 5], a Chinese [6], and a Korean [7], not to mention thousands of other eukaryotic and prokaryotic species sequenced [8]. All these projects only became possible thanks to advanced technologies allowing cost-effective and high-throughput sequencing.[Lander E., Linton L., Birren B., Nusbaum C., Zody M., et al. // Nature 2001. V. 409. PP.860–921.][Levy S., Sutton G., N g P., Feuk L., Halpern A., et al. // PLoS Biology. 2007. Sep 4;5(10):e254.][Wheeler D., Srinivasan M., Egholm M., Shen Y., Chen L., et al. // Nature. 2008. V. 452. PP. 872–876.][Bentley D., Balasubramanian S., Swerdlow H., Smith G., Milton J., et al. // Nature. 2008. V. 456. PP. 53–59.][McKernan K., Peckham H., Costa G., McLaughlin S., Fu Y., et al. // Genome Research. 2009. V. 19. № 9. № 1527–1541.][Wang J., Wang W., Li R., Li Y., Tian G. // Nature. 2008. V. 456. PP. 60 – 65.][Ahn S., Kim T., Lee S., Kim D., Ghang H., et al. // Genome Research. 2009. V. 19. № 9. PP. 1622–1629.][http://www.ncbi.nlm.nih.gov/guide/genomes]