Acta NaturaeActa NaturaeActa Naturae2075-8251Acta Naturae Ltd1078910.32607/20758251-2009-1-3-102-107ArticlesСтатьиResearch ArticleCombining Two Technologies for Full Genome Sequencing of HumanCombining Two Technologies for Full Genome Sequencing of HumanSkryabinK GProkhortchoukE Bprokhortchouk@biengi.ac.ruMazurA MBoulyginaE STsygankovaS VNedoluzhkoA VRastorguevS MMatveevV BChekanovN NGoranskayaD ATeslyukA BGruzdevaN MVelikhovV EZaridzeD GKovalchukM VRussian Research Centre Kurchatov InstituteInstitute of Carcinogenesis, Blokhin Cancer Research Center, Russian Academy of Medical SciencesBioengineering Center, Russian Academy of Sciences1512200913NO3 (2009)№3 (2009)10210717012020Copyright ©; 2009, Skryabin K.G., Prokhortchouk E.B., Mazur A.M., Boulygina E.S., Tsygankova S.V., Nedoluzhko A.V., Rastorguev S.M., Matveev V.B., Chekanov N.N., Goranskaya D.A., Teslyuk A.B., Gruzdeva N.M., Velikhov V.E., Zaridze D.G., Kovalchuk M.V.Copyright ©; 2009, Skryabin K.G., Prokhortchouk E.B., Mazur A.M., Boulygina E.S., Tsygankova S.V., Nedoluzhko A.V., Rastorguev S.M., Matveev V.B., Chekanov N.N., Goranskaya D.A., Teslyuk A.B., Gruzdeva N.M., Velikhov V.E., Zaridze D.G., Kovalchuk M.V.2009Skryabin K.G., Prokhortchouk E.B., Mazur A.M., Boulygina E.S., Tsygankova S.V., Nedoluzhko A.V., Rastorguev S.M., Matveev V.B., Chekanov N.N., Goranskaya D.A., Teslyuk A.B., Gruzdeva N.M., Velikhov V.E., Zaridze D.G., Kovalchuk M.V.Skryabin K.G., Prokhortchouk E.B., Mazur A.M., Boulygina E.S., Tsygankova S.V., Nedoluzhko A.V., Rastorguev S.M., Matveev V.B., Chekanov N.N., Goranskaya D.A., Teslyuk A.B., Gruzdeva N.M., Velikhov V.E., Zaridze D.G., Kovalchuk M.V.https://creativecommons.org/licenses/by/4.0https://actanaturae.ru/2075-8251/article/view/10789

At present, the new technologies of DNA sequencing are rapidly developing allowing quick and efficient characterisation of organisms at the level of the genome structure. In this study, the whole genome sequencing of a human (Russian man) was performed using two technologies currently present on the market - Sequencing by Oligonucleotide Ligation and Detection (SOLiDTM) (Applied Biosystems) and sequencing technologies of molecular clusters using fluorescently labeled precursors (Illumina). The total number of generated data resulted in 108.3 billion base pairs (60.2 billion from Illumina technology and 48.1 billion from SOLiD technology). Statistics performed on reads generated by GAII and SOLiD showed that they covered 75% and 96% of the genome respectively. Short polymorphic regions were detected with comparable accuracy however, the absolute amount of them revealed by SOLiD was several times less than by GAII. Optimal algorithm for using the latest methods of sequencing was established for the analysis of individual human genomes. The study is the first Russian effort towards whole human genome sequencing.

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