Acta NaturaeActa NaturaeActa Naturae2075-8251Acta Naturae Ltd1077910.32607/20758251-2009-1-3-81-83ArticlesСтатьиResearch ArticleD-Arabinose Methabolism: Characterization of Bifunctional Arabinokinase/ Pyrophosphorylase of Leishmania majorD-Arabinose Methabolism: Characterization of Bifunctional Arabinokinase/ Pyrophosphorylase of Leishmania majorNovozhilovaN Mnnovogilov@gmail.comBovinN VShemyakin and Ovchinnikov Institute of Bioorganic Chemistry, RAS1512200913NO3 (2009)№3 (2009)818317012020Copyright ©; 2009, Novozhilova N.M., Bovin N.V.Copyright ©; 2009, Novozhilova N.M., Bovin N.V.2009Novozhilova N.M., Bovin N.V.Novozhilova N.M., Bovin N.V.https://creativecommons.org/licenses/by/4.0https://actanaturae.ru/2075-8251/article/view/10779

In this work we describe an unusual enzyme from Leishmania major (Arabinokinase/Pyrophosphorylase) that catalyzes the synthesis of GDP-Darabinopyranose (GDP-D-Ara p) via a D-arabinose-1-phosphate intermediate in the presence of ATP and GTP. Our data indicate GDP-D-Ara p transport in vivo by the LPG2 multispecific nucleotide sugar transporter into the Leishmania Golgi apparatus, in which it can be used by glycosyltransferases as a donor substrate for glycosylation.

D-arabinopyranoseLeishmaniabifunctional enzymekinasepyriphosphorylaselipophosphoglycanMcConville M.J., Turco S., Ferguson M., Sacks D. // EMBO J. 1992. V. 11. P. 3593–3600.Schneider P., McConville M., Ferguson M. // J Biol Chem. 1994. V. 269. P. 18332–18337.Coyne M., Reinap B., Lee M., Comstock L. // Science. 2005. V. 307. P. 1778–1781.Kotake T., Hojo S., Tajima N., Matsuoka K., Koyama T. et all // JBC. 2008. V. 283. P. 8125–8135.Peneff C., Ferrari P., Charrier V., Taburet Y., Monnier C. et all // EMBO J. 2001. V. 20. P. 6191–6202.Hanks S., Quinn A., Hunter T. // Science. 1988. V. 241. P. 42–52.Hong K., Ma D., Beverley S., Turco S. // Biochemistry. 2000. V. 39. P. 2013–2022.McConville M.J., Bacic A. // JBC. 1989. V. 264. P. 757–766.Spath G., Lye L., Segawa H., Sacks D., Turco S. et all // Science. 2003. V. 301. P. 1241–1243.Ilgoutz S.C., Zawadzki J.L., Ralton J.E., McConville M.J. // EMBO J. 1999. V. 18. P. 2746–2755.