Acta NaturaeActa NaturaeActa Naturae2075-8251Acta Naturae Ltd1076810.32607/20758251-2010-2-2-102-104ArticlesСтатьиShort CommunicationDerivation of Induced Pluripotent Stem Cells from Fetal Human Skin FibroblastsDerivation of Induced Pluripotent Stem Cells from Fetal Human Skin FibroblastsMedvedevS PMalakhovaA AGrigor'evaE VShevchenkoA IDementyevaE VSobolevI ALebedevI NShilovA GZhimulevI FZakianS Mzakian@bionet.nsc.ruInstitute of Cytology and Genetics, Siberian Branch, Russian Academy of SciencesResearch Institute of Medical Genetics, Siberian Branch, Russian Academy of Medical SciencesInstitute of Chemical Biology and Fundamental Medicine, Siberian Branch, Russian Academy of SciencesResearch Center of Clinical and Experimental Medicine, Siberian Branch, Russian Academy of Medical Sciences1506201022VOL 2, NO2 (2010)ТОМ 2, №2 (2010)10210417012020Copyright ©; 2010, Medvedev S.P., Malakhova A.A., Grigor'eva E.V., Shevchenko A.I., Dementyeva E.V., Sobolev I.A., Lebedev I.N., Shilov A.G., Zhimulev I.F., Zakian S.M.Copyright ©; 2010, Medvedev S.P., Malakhova A.A., Grigor'eva E.V., Shevchenko A.I., Dementyeva E.V., Sobolev I.A., Lebedev I.N., Shilov A.G., Zhimulev I.F., Zakian S.M.2010Medvedev S.P., Malakhova A.A., Grigor'eva E.V., Shevchenko A.I., Dementyeva E.V., Sobolev I.A., Lebedev I.N., Shilov A.G., Zhimulev I.F., Zakian S.M.Medvedev S.P., Malakhova A.A., Grigor'eva E.V., Shevchenko A.I., Dementyeva E.V., Sobolev I.A., Lebedev I.N., Shilov A.G., Zhimulev I.F., Zakian S.M.https://creativecommons.org/licenses/by/4.0https://actanaturae.ru/2075-8251/article/view/10768

The isolation and study of autologous human stem cells remain among the most urgent problems in cell biology and biomedicine to date. Induced pluripotent stem cells can be derived from human somatic cells by the overexpression of a number of genes. In this study we reprogrammed fetal human skin fibroblasts by transduction with retroviral vectors carrying murine Oct4, Sox2, Klf4, and c-Myc cDNAs. As a result, cells with the protein expression and gene transcription pattern characteristic of human embryonic stem cells were derived. These induced pluripotent cells are capable of differentiation in vitro into the ectoderm, mesoderm, and endoderm derivatives.

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