Acta NaturaeActa NaturaeActa Naturae2075-8251Acta Naturae Ltd1070510.32607/20758251-2011-3-3-100-106ArticlesСтатьиResearch ArticleAn Efficient Method for the Delivery of the Interleukin-2 Gene to Human Hematopoietic Cells using the Fiber-Modified Recombinant AdenovirusAn Efficient Method for the Delivery of the Interleukin-2 Gene to Human Hematopoietic Cells using the Fiber-Modified Recombinant AdenovirusRogozhinV NRogojin_V@mail.ruLogunovD YuShchebliakovD VShmarovM MKhodunovaE EGaltsevaI VBelousovaR VNaroditskyB SGintsburgA LGamaleya Research Institute of Epidemiology and Microbiology, Ministry of Health and Social Development of the Russian FederationMoscow state academy of veterinary medicine and biotechnology named K.I. SkryabinNational Research Center for Hematology, Ministry of Health and Social Development of the Russian FederationGamaleya Research Institute of Epidemiology and Microbiology, Ministry of Health and Social Development of the Russian Federation1509201133VOL 3, NO3 (2011)ТОМ 3, №3 (2011)10010617012020Copyright ©; 2011, Rogozhin V.N., Logunov D.Y., Shchebliakov D.V., Shmarov M.M., Khodunova E.E., Galtseva I.V., Belousova R.V., Naroditsky B.S., Gintsburg A.L.Copyright ©; 2011, Rogozhin V.N., Logunov D.Y., Shchebliakov D.V., Shmarov M.M., Khodunova E.E., Galtseva I.V., Belousova R.V., Naroditsky B.S., Gintsburg A.L.2011Rogozhin V.N., Logunov D.Y., Shchebliakov D.V., Shmarov M.M., Khodunova E.E., Galtseva I.V., Belousova R.V., Naroditsky B.S., Gintsburg A.L.Rogozhin V.N., Logunov D.Y., Shchebliakov D.V., Shmarov M.M., Khodunova E.E., Galtseva I.V., Belousova R.V., Naroditsky B.S., Gintsburg A.L.https://creativecommons.org/licenses/by/4.0https://actanaturae.ru/2075-8251/article/view/10705

Recombinant human adenovirus serotype 5 (Ad5/35F-IL2) with modified fibres containing the C-terminal domain fiber-knob of human adenovirus serotype 35, carrying the gene of recombinant human IL-2, has been designed. As a result of the fiber modification, the adenovirus can efficiently deliver the genetic information to bone marrow leukocytes and the tumor blood cells KG-1A (human myeloblastic leukemia cells) and U937 (human histiocytic lymphoma cells), which are normally resistant to Ad5 infection. The flow cytometry data reveal that the modified Ad5/35F penetrates into a population of monocytes, granulocytes, and blast cells of human bone marrow. The expression of interleukin-2 in CAR-negative bone marrow leukocytes (3682.52 ± 134.21 pg/ml) and the cell lines KG-1A (748.3 ± 32.8 pg/ml) and U937 (421.5 ± 59.4 pg/ ml) transduced with adenovirus Ad5/35F-IL2 is demonstrated. The fiber-modified adenovirus can be used as a vector for the efficient gene delivery of interleukin-2 to human normal and tumor hematopoietic cells. KEYWORDS adenovial vector; pseudotyping; interleukin-2; CD46; capsid modification.

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