Acta NaturaeActa NaturaeActa Naturae2075-8251Acta Naturae Ltd1070110.32607/20758251-2011-3-1-107-115ArticlesСтатьиResearch ArticleModeling Myocardial Infarction in Mice: Methodology, Monitoring, PathomorphologyModeling Myocardial Infarction in Mice: Methodology, Monitoring, PathomorphologyOvsepyanA APanchenkovD NProkhortchoukE Bprokhortchouk@biengi.ac.ruTeleginG BZhigalovaN AGolubevE PSviridovaT EMatskeplishviliS TSkryabinK GBuziashviliU IThe Branch of the Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Pushchino, Russian Academy of SciencesCenter "Bioengineering", Russian Academy of SciencesMoscow State University of Medicine and DentistryBakoulev Center for Cardiovascular Surgery, Russian Academy of Medical SciencesSemashko Railway HospitalBakoulev Center for Cardiovascular Surgery, Russian Academy of Medical SciencesCenter "Bioengineering", Russian Academy of Sciences1503201131VOL 3, NO1 (2011)ТОМ 3, №1 (2011)10711517012020Copyright ©; 2011, Ovsepyan A.A., Panchenkov D.N., Prokhortchouk E.B., Telegin G.B., Zhigalova N.A., Golubev E.P., Sviridova T.E., Matskeplishvili S.T., Skryabin K.G., Buziashvili U.I.Copyright ©; 2011, Ovsepyan A.A., Panchenkov D.N., Prokhortchouk E.B., Telegin G.B., Zhigalova N.A., Golubev E.P., Sviridova T.E., Matskeplishvili S.T., Skryabin K.G., Buziashvili U.I.2011Ovsepyan A.A., Panchenkov D.N., Prokhortchouk E.B., Telegin G.B., Zhigalova N.A., Golubev E.P., Sviridova T.E., Matskeplishvili S.T., Skryabin K.G., Buziashvili U.I.Ovsepyan A.A., Panchenkov D.N., Prokhortchouk E.B., Telegin G.B., Zhigalova N.A., Golubev E.P., Sviridova T.E., Matskeplishvili S.T., Skryabin K.G., Buziashvili U.I.https://creativecommons.org/licenses/by/4.0https://actanaturae.ru/2075-8251/article/view/10701

Myocardial infarction is one of the most serious and widespread diseases in the world. In this work, a minimally invasive method for simulating myocardial infarction in mice is described in the Russian Federation for the very first time; the procedure is carried out by ligation of the coronary heart artery or by controlled electrocoagulation. As a part of the methodology, a series of anesthetic, microsurgical and revival protocols are designed, owing to which a decrease in the postoperational mortality from the initial 94.6 to 13.6% is achieved. ECG confirms the development of large-focal or surface myocardial infarction. Postmortal histological examination confirms the presence of necrosis foci in the heart muscles of 87.5% of animals. Altogether, the medical data allow us to conclude that an adequate mouse model for myocardial infarction was generated. A further study is focused on the standardization of the experimental procedure and the use of genetically modified mouse strains, with the purpose of finding the most efficient therapeutic approaches for this disease.

myocardial infarctioncoronary arteryligationcontrolled electrocoagulationECG (electrocardiogram)Burgueno A.L., Landa M.S., Schuman M.L., Alvarez A.L., Carabelli J., Garcia S.I., Pirola C.J. // Metabolism. 2007. V. N 10. P. 1439-1443.Cascio W.E., Cozzi E., Hazarika S., Devlin R.B., Henriksen R.A., Lust R.M., van Scott M.R., Wingard C.J. // Inhal Toxicol. 2007. V. 19. P. 67-73.Matsuzawa N., Takamura T., Kurita S., Misu H., Ota T., Ando H., Yokoyama M., Honda M., Zen Y., Nakanuma Y., et al. // Hepatology. 2007. V. 46. N 5. P. 1392-1403.Cohen J.K., Cai L.Q., Zhu Y.S., La Perle K.M. // Comp. Med. 2007. V. 57. N4. P. 370-376.Denvir M.A., Sharif I., Anderson T., Webb D.J., Gray G.A., McDicken W.N. // J. Am. Soc. Echocardiogr. 2005. V. 18. N 2. P. 155-162.Lee S., Schwinger R.H., Brixius K. // Pflugers Arch. 2008. V. 455. N 5. P. 767-774.Moura R., Tjwa M., Vandervoort P., Cludts K., Hoylaerts M.F. // Arterioscler. Thromb. Vasc. Biol. 2007. V. 27. N 10. P. 2163-2169.Nogueira B.V., Peotta V.A., Meyrelles S.S., Vasquez E.C. // Arch. Med. Res. 2007. V. 38. N 8. P. 816-821.Rajasingh J., Bord E., Hamada H., Lambers E., Qin G., Losordo D.W., Kishore R. // Vet. Res. Commun. 2007. V. 31. P. 35-41.Simpson E.R., Jones M.E. // Ernst Schering Found. Symp. Proc. 2006. V. 1. P. 45-67.Son N.H., Park T.S., Yamashita H., Yokoyama M., Huggins L.A., Okajima K., Homma S., Szabolcs M.J., Huang L.S., Goldberg I.J. // J. Clin. Invest. 2007. V. 117. N 10. P. 2791-2801.Tang Y., Liu W., Yu S., Wang Y., Peng Q., Xiong Z., Wang Y., Wei T. // Plast. Reconstr. Surg. 2007. V. 120. N 4. P. 869-878.Wang G.S., Kearney D.L., De Biasi M., Taffet G., Cooper T.A. // J. Clin. Invest. 2007. V. 117. N 10. P. 2802-2811.Smolyannikov A. V., Naddachina T. A. Patologicheskaya anatomiya koronarnoi nedostatochnosti (Pathological Anatomy of Coronary Insufficiency). M.: Meditsina, 1963.