Acta NaturaeActa NaturaeActa Naturae2075-8251Acta Naturae Ltd1068510.32607/20758251-2011-3-3-64-70ArticlesСтатьиResearch ArticleEffective Genetic Expression of Nanoantibodies by Recombinant Adenoviral Vector in vitroEffective Genetic Expression of Nanoantibodies by Recombinant Adenoviral Vector in vitroGribovaI YuTillibS VTutykhinaI LShmarovМ МLogunovD YuVerkhovskayaL VNaroditskiiB Sbsnar1941@yahoo.comGintsburgA LGamaleya Research Institute for Epidemiology and MicrobiologyInstitute of Gene Biology, Russian Academy of Sciences1509201133VOL 3, NO3 (2011)ТОМ 3, №3 (2011)647017012020Copyright ©; 2011, Gribova I.Y., Tillib S.V., Tutykhina I.L., Shmarov М.М., Logunov D.Y., Verkhovskaya L.V., Naroditskii B.S., Gintsburg A.L.Copyright ©; 2011, Gribova I.Y., Tillib S.V., Tutykhina I.L., Shmarov М.М., Logunov D.Y., Verkhovskaya L.V., Naroditskii B.S., Gintsburg A.L.2011Gribova I.Y., Tillib S.V., Tutykhina I.L., Shmarov М.М., Logunov D.Y., Verkhovskaya L.V., Naroditskii B.S., Gintsburg A.L.Gribova I.Y., Tillib S.V., Tutykhina I.L., Shmarov М.М., Logunov D.Y., Verkhovskaya L.V., Naroditskii B.S., Gintsburg A.L.https://creativecommons.org/licenses/by/4.0https://actanaturae.ru/2075-8251/article/view/10685

The present study is devoted to the feasibility of expressing the single-domain mini-antibody (nanoantibody) selected from the library of sequences of the variable domains of special single-stranded antibodies derived from an immunized camel, a gene of which was introduced into eukaryotic cells within a recombinant adenoviral vector. A vector bearing the gene of a single-domain nanoantibody was obtained using the AdEasy Adenoviral Vector System (Stratagene). This method of delivering the nanoantibody gene facilitates efficient expression of this gene and functional activity of the nanoantibody. The results obtained can be used to produce passive immunizing tools against pathogens or new-generation immunobiological antitoxic medication.

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