Acta NaturaeActa NaturaeActa Naturae2075-8251Acta Naturae Ltd1066110.32607/20758251-2011-3-4-100-106ArticlesСтатьиResearch ArticleThe Fate of the Nucleolus during Mitosis: Comparative Analysis of Localization of Some Forms of Pre-rRNA by Fluorescent in Situ Hybridization in NIH/3T3 Mouse FibroblastsThe Fate of the Nucleolus during Mitosis: Comparative Analysis of Localization of Some Forms of Pre-rRNA by Fluorescent in Situ Hybridization in NIH/3T3 Mouse FibroblastsShishovaK Vkseniya.shishova@inbox.ruZharskayaО ОZatsepinaОShemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences1512201134VOL 3, NO4 (2011)ТОМ 3, №4 (2011)10010617012020Copyright ©; 2011, Shishova K.V., Zharskaya О.О., Zatsepina О.Copyright ©; 2011, Shishova K.V., Zharskaya О.О., Zatsepina О.2011Shishova K.V., Zharskaya О.О., Zatsepina О.Shishova K.V., Zharskaya О.О., Zatsepina О.https://creativecommons.org/licenses/by/4.0https://actanaturae.ru/2075-8251/article/view/10661

Nucleolus is the major structural domain of the cell nucleus, which in addition to proteins contains ribosomal RNA (rRNA) at different stages of maturation (or pre-rRNA). In mammals, the onset of mitosis is accompanied by the inhibition of rRNA synthesis, nucleolus disassembly, and the migration of pre-rRNA to the cytoplasm. However, the precise role of cytoplasmic pre-rRNA in mitosis remains unclear, and no comparative analysis of its different forms at consequent mitotic stages has thus far been performed. The focus of this research was the study of the localization of pre-rRNA in mitotic NIH/3T3 mouse fibroblasts by fluorescent in situ hybridization (FISH) with probes to several regions of mouse primary 47S pre-rRNA transcripts and by confocal laser microscopy. The results reveal that all types of pre-rRNA appear in the cytoplasm at the beginning of mitosis, following the breakdown of the nucleolus and nuclear envelope. However, not all pre-rRNA are transported by chromosomes from maternal cells into daughter cells. At the end of mitosis, all types of pre-rRNA and 28S rRNA can be visualized in nucleolus-derived foci (NDF), structures containing many proteins of mature nucleoli the appearance of which indicates the commencement of nucleologenesis. However, early NDF are enriched in less processed pre-RNA, whereas late NDF contain predominantly 28S rRNA. Altogether, the results of this study strengthen the hypotheses that postulate that different forms of pre-rRNA may play various roles in mitosis, and that NDF can be involved in the maturation of pre-rRNA, remaining preserved in the cytoplasm of dividing cells.

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