Acta NaturaeActa Naturae2075-8251Acta Naturae Ltd1053310.32607/20758251-2014-6-3-41-51Research ArticleMycoplasma Contamination of Cell Cultures: Vesicular Traffic in Bacteria and Control over Infectious AgentsChernovV. M.ljoscha@mail.ruChernovaO. A.ljoscha@mail.ruSanchez-VegaJ. T.ljoscha@mail.ruKolpakovА. I.ljoscha@mail.ruIlinskayaО. N.ljoscha@mail.ruKazan Institute of Biochemistry and Biophysics, Kazan Scientific Center, Russian Academy of SciencesKazan (Volga Region) Federal UniversityNational Autonomous University of Mexico1509201463415117012020Copyright © 2014, Chernov V.M., Chernova O.A., Sanchez-Vega J.T., Kolpakov А.I., Ilinskaya О.N.2014<p>Cell cultures are subject to contamination either with cells of other cultures or with microorganisms, including fungi, viruses, and bacteria. Mycoplasma contamination of cell cultures is of particular importance. Since cell cultures are used for the production of vaccines and physiologically active compounds, designing a system for controlling contaminants becomes topical for fundamental science and biotechnological production. The discovery of extracellular membrane vesicles in mycoplasmas makes it necessary to take into consideration the bacterial vesicular traffic in systems designed for controlling infectious agents. The extracellular vesicles of bacteria mediate the traffic of proteins and genes, participate in cell-to-cell interactions, as well as in the pathogenesis and development of resistance to antibiotics. The present review discusses the features of mycoplasmas, their extracellular vesicles, and the interaction between contaminants and eukaryotic cells. Furthermore, it provides an analysis of the problems associated with modern methods of diagnosis and eradication of mycoplasma contamination from cell cultures and prospects for their solution.</p>diagnosis and eradicationcell culturesmycoplasma contaminationДиагностика и эрадикацияклеточные культурымикоплазменная контаминация[[1] Borkhsenius S.N., Chernova O.A., Chernov V.M., Vonsky M.S. // Mycoplasma. St. Petersburg.: Science. 2002. 2002, P.320][[2] Uphoff C., Drexler H. // Meth. Mol. Biol. 2011, V.731, P.105-114][[3] Rottem S., Kosower N.S., Kornspan J.D. // Contamination of Tissue Cultures by Mycoplasmas, Biomedical Tissue Culture / Ed. 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