Acta NaturaeActa NaturaeActa Naturae2075-8251Acta Naturae Ltd1031010.32607/20758251-2019-11-1-81-90Research ArticlesЭкспериментальные статьиResearch ArticleMauritian Endemic Medicinal Plant Extracts Induce G2/M Phase Cell Cycle Arrest and Growth Inhibition of Oesophageal Squamous Cell Carcinoma in VitroMauritian Endemic Medicinal Plant Extracts Induce G2/M Phase Cell Cycle Arrest and Growth Inhibition of Oesophageal Squamous Cell Carcinoma in VitroRummunN.
Mauritius
v.neergheen@uom.ac.muHughesR. E.
United Kingdom
v.neergheen@uom.ac.muBeesooR.
Mauritius
v.neergheen@uom.ac.muLiW. W.
United Kingdom
v.neergheen@uom.ac.muAldulaimiO.
United Kingdom
v.neergheen@uom.ac.muMacleodK. G.
United Kingdom
v.neergheen@uom.ac.muBahorunT.
Mauritius
v.neergheen@uom.ac.muCarragherN. O.
United Kingdom
N.Carragher@ed.ac.ukKaganskyA.
United Kingdom
kagasha@yahoo.comNeergheen-BhujunV. S.
Mauritius
v.neergheen@uom.ac.muUniversity of MauritiusANDI Centre of Excellence for Biomedical and Biomaterials ResearchInstitute for Science and Technology in MedicineCancer Research UK Edinburgh Centre, University of EdinburghUniversity of EdinburghUniversity of EdinburghANDI Centre of Excellence for Biomedical and Biomaterials ResearchFar Eastern Federal UniversityUniversity of Mauritius15032019111VOL 11, NO1 (2019)ТОМ 11, №1 (2019)819017012020Copyright ©; 2019, Rummun N., Hughes R.E., Beesoo R., Li W.W., Aldulaimi O., Macleod K.G., Bahorun T., Carragher N.O., Kagansky A., Neergheen-Bhujun V.S.Copyright ©; 2019, Rummun N., Hughes R.E., Beesoo R., Li W.W., Aldulaimi O., Macleod K.G., Bahorun T., Carragher N.O., Kagansky A., Neergheen-Bhujun V.S.2019Rummun N., Hughes R.E., Beesoo R., Li W.W., Aldulaimi O., Macleod K.G., Bahorun T., Carragher N.O., Kagansky A., Neergheen-Bhujun V.S.Rummun N., Hughes R.E., Beesoo R., Li W.W., Aldulaimi O., Macleod K.G., Bahorun T., Carragher N.O., Kagansky A., Neergheen-Bhujun V.S.https://creativecommons.org/licenses/by/4.0https://actanaturae.ru/2075-8251/article/view/10310

Terrestrial plants have contributed massively to the development of modern oncologic drugs. Despite the wide acceptance of Mauritian endemic flowering plants in traditional medicine, scientific evidence of their chemotherapeutic potential is lacking. This study aimed to evaluate the in vitro tumor cytotoxicity of leaf extracts from five Mauritian endemic medicinal plants, namely Acalypha integrifolia Willd (Euphorbiaceae), Labourdonnaisia glauca Bojer (Sapotaceae), Dombeya acutangula Cav. subsp. rosea Friedmann (Malvaceae), Gaertnera psychotrioides (DC.) Baker (Rubiaceae), and Eugenia tinifolia Lam (Myrtaceae).rolex oyster perpetual day date real or fake The cytotoxicities of the extracts were determined against six human cancer cell lines, including cervical adenocarcinoma, colorectal carcinoma, oesophageal adenocarcinoma, and oesophageal squamous cell carcinoma. The potent extracts were further investigated using cell cycle analysis and reverse phase protein array (RPPA) analysis. The antioxidant properties and polyphenolic profile of the potent extracts were also evaluated. Gas chromatography mass spectrometry (GC-MS) analyses revealed the presence of (+)-catechin and gallocatechin in E. tinifolia and L. glauca, while gallic acid was detected in A. integrifolia. L. glauca, A. integrifolia, and E. tinifolia were highly selective towards human oesophageal squamous cell carcinoma (KYSE-30) cells. L. glauca and E. tinifolia arrested KYSE- 30 cells in the G2/M phase, in a concentration-dependent manner. RPPA analysis indicated rolex fake that the extracts may partly exert their tumor growth-inhibitory activity by upregulating the intracellular level of 5′AMP-activated kinase (AMPK). The findings highlight the potent antiproliferative activity of three Mauritian endemic leaf extracts against oesophageal squamous ccheap replica luxury watches ell carcinoma and calls for further investigation into their chemotherapeutic application.

Terrestrial plants have contributed massively to the development of modern oncologic drugs. Despite the wide acceptance of Mauritian endemic flowering plants in traditional medicine, scientific evidence of their chemotherapeutic potential is lacking. This study aimed to evaluate the in vitro tumor cytotoxicity of leaf extracts from five Mauritian endemic medicinal plants, namely Acalypha integrifolia Willd (Euphorbiaceae), Labourdonnaisia glauca Bojer (Sapotaceae), Dombeya acutangula Cav. subsp. rosea Friedmann (Malvaceae), Gaertnera psychotrioides (DC.) Baker (Rubiaceae), and Eugenia tinifolia Lam (Myrtaceae). The cytotoxicities of the extracts were determined against six human cancer cell lines, including cervical adenocarcinoma, colorectal carcinoma, oesophageal adenocarcinoma, and oesophageal squamous cell carcinoma. The potent extracts were further investigated using cell cycle analysis and reverse phase protein array (RPPA) analysis. The antioxidant properties and polyphenolic profile of the potent extracts were also evaluated. Gas chromatography mass spectrometry (GC-MS) analyses revealed the presence of (+)-catechin and gallocatechin in E. tinifolia and L. glauca, while gallic acid was detected in A. integrifolia. L. glauca, A. integrifolia, and E. tinifolia were highly selective towards human oesophageal squamous cell carcinoma (KYSE-30) cells. L. glauca and E. tinifolia arrested KYSE- 30 cells in the G2/M phase, in a concentration-dependent manner. RPPA analysis indicated that the extracts may partly exert their tumor growth-inhibitory activity by upregulating the intracellular level of 5′AMP-activated kinase (AMPK). The findings highlight the potent antiproliferative activity of three Mauritian endemic leaf extracts against oesophageal squamous cell carcinoma and calls for further investigation into their chemotherapeutic application.

Mauritian endemicmedicinal plantoesophageal carcinomatumor cytotoxicityAMPK[1] Pennathur A., Gibson M.K., Jobe B.A., Luketich J.D. // Lancet. 2013, V.381, №9864, P.400-412[2] Napier K.J. // World J. Gastrointest. Oncol. 2014, V.6, №5, P.112[3] Willis K.J. // State of the World’s Plants 2017[4] Graham J.G., Quinn M.L., Fabricant D.S., Farnsworth N.R. // J. Ethnopharmacol. 2000, V.73, №3, P.347-377[5] Rummun N., Neergheen-Bhujun V.S., Pynee K.B., Baider C., Bahorun T. // J. Ethnopharmacol. 2018, V.2013, №April, P.111-117[6] Rummun N., Somanah J., Ramsaha S., Bahorun T., Neergheen-Bhujun V.S. // Int. J. Food Sci. 2013, V.2013, P.1-12[7] Johnson-Ajinwo O.R., Richardson A., Li W.W. // Phytomedicine. 2015, V.22, №1, P.1-4[8] Chu W.L., Lim Y.W., Radhakrishnan A.K., Lim P.E. // BMC Complement. Altern. Med. 2010, V.10, №53[9] Macleod K.G., Serrels B., Carragher N.O. // Proteomics for Drug Discovery. New York: Springer, 2017, P.153-169[10] Mathur S., Hoskins C. // Biomed. Reports. 2017, V.6, №6, P.612-614[11] Grzesik M., Naparło K., Bartosz G., Sadowska-Bartosz I. // Food Chem. 2018. 2017, V.241, №June, P.480-492[12] Bahorun T., Luximon-Ramma A., Crozier A., Aruoma O.I. // J. Sci. Food Agric. 2004, V.84, №12, P.1553-1561[13] Mersch-Sundermann V., Bahorun T., Stahl T., Neergheen V.S., Soobrattee M.A., Wohlfarth R., Sobel R., Brunn H.E., Schmeiser T., Lamy E. // Toxicol. Vitr. 2006, V.20, №8, P.1427-1434[14] Luximon-Ramma A., Neergheen V.S., Bahorun T., Crozier A., Zbarsky V., Datla K.P., Dexter D.T., Aruoma O.I. // Biofactors. 2006, V.27, №1-4, P.79-91[15] Neergheen V.S., Bahorun T., Jen L.S., Aruoma O.I. // Pharm. Biol. 2007, V.45, №1, P.9-17[16] Soobrattee M.A., Bahorun T., Thaunoo P. // Food Agric. Res. Counc. Réduit, Mauritius. 2005, P.13-21[17] Soobrattee M.A., Bahorun T., Neergheen V.S., Googoolye K., Aruoma O.I. // Toxicol. Vitr. 2008, V.22, №1, P.45-56[18] Neergheen V.S., Soobrattee M.A., Bahorun T., Aruoma O.I. // J. Plant Physiol. 2006, V.163, №8, P.787-799[19] Soobrattee M.A., Neergheen V.S., Luximon-Ramma A., Aruoma O.I., Bahorun T. // Mutat. Res. Mol. Mech. Mutagen. 2005, V.579, №1-2, P.200-213[20] San Miguel-Chávez R. // In: Phenolic Compounds - Biological Activity. 2017, P.59-74[21] Cuvelier M.-E., Richard H., Berset C. // Biosci. Biotechnol. Biochem. 1992, V.56, №2, P.324-325[22] Procházková D., Boušová I., Wilhelmová N. // Fitoterapia. 2011, V.82, №4, P.513-523[23] Huang D., Ou B., Prior R.L. // J. Agric. Food Chem. 2005, V.53, №6, P.1841-1856[24] Ramful D., Tarnus E., Rondeau P., Da Silva C.R., Bahorun T., Bourdon E. // J. Agric. Food Chem. 2010, V.58, №20, P.11119-11129[25] Toyokuni S., Okamoto K., Yodoi J., Hiai H. // FEBS Lett. 1995, V.358, №1, P.1-3[26] Soobrattee M.A., Bahorun T., Aruoma O.I. // Biofactors. 2006, V.27, №1-4, P.19-35[27] Neergheen V., Bahorun T., Aruoma O.I. // Univ. Mauritius Res. J. 2011, V.17, P.240-255[28] Ramos-Silva A., Tavares-Carreón F., Figueroa M., De la Torre-Zavala S., Gastelum-Arellanez A., Rodríguez-García A., Galán-Wong L.J., Avilés-Arnaut H. // BMC Complement. Altern. Med. 2017, V.17, №1, P.241[29] Sever R., Brugge J.S. // Cold Spring Harb. Perspect. Med. 2015, V.5, №4, a006098[30] Li W., Saud S.M., Young M.R., Chen G., Hua B. // Oncotarget. 2015, V.6, №10, P.7365-7378[31] Cai X., Hu X., Tan X., Cheng W., Wang Q., Chen X., Guan Y., Chen C., Jing X. // PLoS One. 2015, V.10, №7, e0133349[32] Wu P., Liu S., Su J., Chen J., Li L., Zhang R., Chen T. // Food Funct. 2017, V.8, №10, P.3707-3722[33] Xiao J., Niu G., Yin S., Xie S., Li Y., Nie D., Ma L., Wang X., Wu Y. // Acta Biochim. Biophys. Sin. (Shanghai). 2014, V.46, №5, P.394-400