Email this article Combining Two Technologies for Full Genome Sequencing of Human
- Authors: Skryabin KG1, Prokhortchouk EB1, Mazur AM1, Boulygina ES1, Tsygankova SV1, Nedoluzhko AV1, Rastorguev SM1, Matveev VB2, Chekanov NN3, Goranskaya DA3, Teslyuk AB1, Gruzdeva NM1, Velikhov VE1, Zaridze DG2, Kovalchuk MV1
-
Affiliations:
- Russian Research Centre Kurchatov Institute
- Institute of Carcinogenesis, Blokhin Cancer Research Center, Russian Academy of Medical Sciences
- Bioengineering Center, Russian Academy of Sciences
- Issue: Vol 1, No 3 (2009)
- Pages: 102-107
- Section: Articles
- URL: https://actanaturae.ru/2075-8251/article/view/10789
- DOI: https://doi.org/10.32607/20758251-2009-1-3-102-107
- ID: 10789
Cite item
Abstract
Full Text
Progress in the development of novel DNA sequencing technologies allowing rapid and accurate determination of the distinctive features of an individual at the level of the structure of his genome has made genomics one of the most rapidly developing scientific disciplines. At present, three advanced technologies of DNA sequencing are in use: pyrosequencing based on the “sequencing-by-synthesis” principle and commercialized in a next-generation Roche GS-FLX capillary genome sequencing system (454 Life Science Inc./Roche), cyclic sequencing by oligonucleotide ligation and detection (SOLiD, Applied Biosystems), and high-throughput DNA molecular cluster sequencing-by-synthesis using proprietary fluorescently labeled oligonucleotides (Illumina GAII Genome Analyzer, previously known as SOLEXA). These platforms have already demonstrated their significant worth: in the last two years, five new genomes have been read – in addition to the reference genome sequence that was determined by several leading research groups at institutes in the U.S., United Kingdom, and Canada in the course of 10 years and at a cost of 3 bln dollars [1] – such as the genomes of outstanding biologists of our times[2, 3], that of a Nigerian man [4, 5], a Chinese [6], and a Korean [7], not to mention thousands of other eukaryotic and prokaryotic species sequenced [8]. All these projects only became possible thanks to advanced technologies allowing cost-effective and high-throughput sequencing.About the authors
K G Skryabin
Russian Research Centre Kurchatov Institute
E B Prokhortchouk
Russian Research Centre Kurchatov Institute
Email: prokhortchouk@biengi.ac.ru
A M Mazur
Russian Research Centre Kurchatov Institute
E S Boulygina
Russian Research Centre Kurchatov Institute
S V Tsygankova
Russian Research Centre Kurchatov Institute
A V Nedoluzhko
Russian Research Centre Kurchatov Institute
S M Rastorguev
Russian Research Centre Kurchatov Institute
V B Matveev
Institute of Carcinogenesis, Blokhin Cancer Research Center, Russian Academy of Medical Sciences
N N Chekanov
Bioengineering Center, Russian Academy of Sciences
D A Goranskaya
Bioengineering Center, Russian Academy of Sciences
A B Teslyuk
Russian Research Centre Kurchatov Institute
N M Gruzdeva
Russian Research Centre Kurchatov Institute
V E Velikhov
Russian Research Centre Kurchatov Institute
D G Zaridze
Institute of Carcinogenesis, Blokhin Cancer Research Center, Russian Academy of Medical Sciences
M V Kovalchuk
Russian Research Centre Kurchatov Institute
References
- Lander E., Linton L., Birren B., Nusbaum C., Zody M., et al. // Nature 2001. V. 409. PP.860–921.
- Levy S., Sutton G., N g P., Feuk L., Halpern A., et al. // PLoS Biology. 2007. Sep 4;5(10):e254.
- Wheeler D., Srinivasan M., Egholm M., Shen Y., Chen L., et al. // Nature. 2008. V. 452. PP. 872–876.
- Bentley D., Balasubramanian S., Swerdlow H., Smith G., Milton J., et al. // Nature. 2008. V. 456. PP. 53–59.
- McKernan K., Peckham H., Costa G., McLaughlin S., Fu Y., et al. // Genome Research. 2009. V. 19. № 9. № 1527–1541.
- Wang J., Wang W., Li R., Li Y., Tian G. // Nature. 2008. V. 456. PP. 60 – 65.
- Ahn S., Kim T., Lee S., Kim D., Ghang H., et al. // Genome Research. 2009. V. 19. № 9. PP. 1622–1629.
- http://www.ncbi.nlm.nih.gov/guide/genomes
Supplementary files
